Article
Microbiology
Ruikang Liu, Jeffrey L. Americo, Patricia L. Earl, Jack Villani, Catherine A. Cotter, Bernard Moss
Summary: The more pathogenic Clone 3 (CL3) virus compared to ACAM2000 is attributed to the presence of a full-length IFN-alpha/beta decoy receptor in CL3 and a truncation of the receptor in ACAM2000. Experimental evidence shows that viruses expressing the full-length decoy receptor cause more severe diseases in mice.
Article
Medicine, General & Internal
Shuguang Zuo, Min Wei, Bohao He, Anxian Chen, Shiqun Wang, Lingkai Kong, Yenan Zhang, Gang Meng, Tiancheng Xu, Jingyi Wu, Fuming Yang, Hailin Zhang, Shibing Wang, Ciliang Guo, Junhua Wu, Jie Dong, Jiwu Wei
Summary: The study demonstrates that VV-alpha-TIGIT exhibits effective anti-tumor efficacy in various tumor models, particularly achieving approximately 70% complete tumor regression in an ascites tumor model. Treatment with VV-alpha-TIGIT also significantly increases the recruitment and activation of T cells in TME.
Article
Microbiology
Ruikang Liu, Jeffrey L. Americo, Patricia L. Earl, Jack Villani, Catherine A. Cotter, Bernard Moss
Summary: The difference in pathogenicity between the pathogenic isolate clone 3 (CL3) and the second-generation smallpox vaccine ACAM2000 is associated with truncation of the interferon alpha/beta (IFN-alpha/beta) decoy receptor. Viruses expressing the full-length decoy receptor are more virulent in mouse models.
Article
Virology
Pin-Han Chen, Tsai-Ching Hsueh, Jen-Leih Wu, Jiann-Ruey Hong
Summary: This study demonstrates that infectious spleen and kidney necrosis virus (ISKNV) infection can induce host cell death through the regulation of mitochondrial membrane potential. The dynamic interaction between pro-apoptotic and anti-apoptotic members of the Bcl-2 family plays a crucial role in this process.
Review
Biochemistry & Molecular Biology
Zhilong Yang, Mark Gray, Lake Winter
Summary: Poxviruses are still significant public health concerns and serious endemic diseases despite the eradication of smallpox. They have shaped modern medicine, provided insights into complex life processes, and are utilized in biotechniques and fighting other infectious diseases and cancers. Continued strong support for poxvirus research is crucial for expanding fundamental biological knowledge and battling diverse diseases.
CELL AND BIOSCIENCE
(2021)
Article
Multidisciplinary Sciences
R. V. Sriram Uday, Rajdip Misra, Annaram Harika, Sandip Dolui, Achintya Saha, Uttam Pal, V. Ravichandiran, Nakul C. Maiti
Summary: Some cancer treatment drugs can bind to the non-active site of the dengue virus NS3 protease, affecting the protein's conformation and dynamics, potentially altering the enzyme's functionality and activity.
Article
Microbiology
Eva Mittler, Tanwee Alkutkar, Rohit K. Jangra, Kartik Chandran
Summary: Researchers introduced a novel in situ assay to monitor the binding of GPCL-NPC1 in intact, infected cells. This assay, based on DNA-assisted, antibody-mediated proximity ligation, allows for visualization of binding complexes and helps dissect the interaction between the Ebola virus glycoprotein and its receptor NPC1.
Article
Biophysics
Arianna Mencattini, Christine Lansche, Irina Veith, Philippe Erbs, Jean-Marc Balloul, Eric Quemeneur, Stephanie Descroix, Fatima Mechta-Grigoriou, Gerard Zalcman, Cecile Zaupa, Maria Carla Parrini, Eugenio Martinelli
Summary: Organ-on-chip and tumor-on-chip microfluidic cell cultures are important for modeling organ functions, diseases, drug development, and personalized medicine. Analyzing the large amount of bio-images acquired in these 3D microenvironments is a challenge, which can be addressed by the interdisciplinary contributions of computer science and electronic engineering. In this study, we applied this approach to study the oncolytic vaccinia virus (OVV) in cancer immunotherapy, and found a synergistic cooperation between OVV and immune cells in killing cancer cells. We also observed changes in immune cell kinetics and a correlation between cancer cell infection and cancer-immune interaction time.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Oncology
Zeinab Babaei, Mojtaba Panjehpour, Hadi Parsian, Mahmoud Aghaei
Summary: The study found that SAR131675 can inhibit the proliferation of human endothelial cells and induce cell apoptosis. The results also indicated that SAR131675 induced apoptosis by affecting mitochondrial membrane potential, generating reactive oxygen species, and regulating the expression of Bcl-2/Bax/Cyto c, leading to the inhibition of cancer cell proliferation.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Virology
Leon C. W. Lin, Sarah N. Croft, Nathan P. Croft, Yik Chun Wong, Stewart A. Smith, Swee-Seong Tang, Anthony W. Purcell, David C. Tscharke
Summary: The study investigated the CD8(+) T cell priming pathways for Vaccinia virus (VACV) by engineering the virus to express inhibitors of antigen presentation. While the inhibitors had diverse impacts on CD8(+) T cell responses, unexpectedly, the direct priming of CD8(+) T cells by poxviruses was robust enough to withstand potent viral inhibitors of antigen presentation. This demonstrates the flexibility and robustness of immune processes in activating immune responses required to fight infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Markus Rose, Martin Kurylowicz, Mohammad Mahmood, Sheldon Winkel, Jose M. Moran-Mirabal, Cecile Fradin
Summary: This study focuses on the interaction between two core members of the Bcl-2 family, Bax and tBid, revealing their different conformations on the membrane and their increased affinity when transitioning from loosely membrane-associated to transmembrane forms. The researchers used single particle imaging and cross-correlation analysis to infer protein conformation and detect transient interactions in a mitochondria-like planar supported lipid bilayer. This leads to an updated molecular model for the activation of Bax by tBid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Chinthakunta Narendra Reddy, Ramasubbu Sankararamakrishnan
Summary: Viruses have evolved strategies to prevent apoptosis of infected cells, with proteins like A179L and BHRF1 showing favorable interactions with BH3-like peptides. The design of specific BH3-mimetic inhibitors for vBcl-2 homologues must also consider long-range interactions and nonhotspot residues.
Review
Chemistry, Multidisciplinary
Ashley N. Matthew, Florian Leidner, Gordon J. Lockbaum, Mina Henes, Jacqueto Zephyr, Shurong Hou, Desaboini Nageswara Rao, Jennifer Timm, Linah N. Rusere, Debra A. Ragland, Janet L. Paulsen, Kristina Prachanronarong, Djade Soumana, Ellen A. Nalivaika, Nese Kurt Yilmaz, Akbar Ali, Celia A. Schiffer
Summary: The article discusses the prevalence of drug resistance across various diseases and emphasizes the importance of proactive strategies in drug design and development to minimize the impact of resistance. It also highlights the lessons learned from antiviral drug resistance mechanisms and rational strategies to avoid resistance in drug design.
Article
Virology
Lin Yang, Chenyu Wang, Jinqi Shu, Huapeng Feng, Yulong He, Jian Chen, Jianhong Shu
Summary: In studying the pathogenic mechanism of PEDV, it was found that PEDV infection can activate Puma and induce apoptosis in a P53-dependent pathway. These findings provide valuable data support for further research on the pathogenic mechanism of PEDV and the development of an effective vaccine against PEDV.
Article
Chemistry, Medicinal
Xiaonan Dong, Qiren Liang, Yun-Zu Pan, Xiaoyu Wang, Yi-Chun Kuo, Wei-Chung Chiang, Xuewu Zhang, Noelle S. Williams, Josep Rizo, Beth Levine, Jef K. De Brabander
Summary: This study demonstrates the development of small molecule compounds that selectively release the inhibition of autophagy-related protein Beclin 1 by Bcl-2. By targeting protein-protein interactions of the intrinsic autophagy regulatory network, this research provides a valuable strategy for the development of autophagy-based therapeutics.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Nutrition & Dietetics
Claire Gallagher, George Moschonis, Katrina A. Lambert, Eva Karaglani, Christina Mavrogianni, Stavroula Gavrili, Yannis Manios, Bircan Erbas
Summary: The study found that high sugar-sweetened beverage consumption is associated with visceral fat in schoolchildren aged 9-13, especially in boys. Additionally, children with lower morning serum cortisol levels are more likely to be affected by sugar-sweetened beverages.
BRITISH JOURNAL OF NUTRITION
(2021)
Review
Cell Biology
Miguel Hernandez-Gonzalez, Gabrielle Larocque, Michael Way
Summary: Membrane trafficking is a crucial cellular process that influences various aspects of cell function, and its dysfunction is associated with serious genetic, metabolic, and neurological disorders. Viruses often hijack membrane trafficking during infection to facilitate their replication, assembly, and egress.
JOURNAL OF CELL SCIENCE
(2021)
Editorial Material
Cell Biology
Michael Way, Sharon Ahmad
JOURNAL OF CELL SCIENCE
(2021)
Editorial Material
Cell Biology
Michael Way
JOURNAL OF CELL SCIENCE
(2021)
Editorial Material
Cell Biology
Michael Way, Sharon Ahmad
JOURNAL OF CELL SCIENCE
(2022)
Editorial Material
Cell Biology
Sharon Ahmad, Michael Way
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Angika Basant, Michael Way
Summary: The positioning of pTyr motifs plays a crucial role in the signal cascade driving the movement and spread of Vaccinia virus. Only specific spatial arrangements of pTyr motifs result in the recruitment of N-WASP and Arp2/3 complex-mediated actin polymerization, indicating that the relative position of pTyr adaptor binding sites is optimized for signal output.
Article
Cell Biology
Amadeus Xu, Angika Basant, Sibylle Schleich, Timothy P. Newsome, Michael Way
Summary: The study reveals that intracellular mature viruses (IMVs) and intracellular enveloped viruses (IEVs) can undergo microtubule-based motility and recruit the kinesin-1 motor protein for transport. IMVs are the first and most abundant infectious form of vaccinia virus during its replication cycle. These findings provide insights into the transport mechanism of the virus.
JOURNAL OF CELL SCIENCE
(2023)
Editorial Material
Cell Biology
Michael Way
JOURNAL OF CELL SCIENCE
(2022)
Editorial Material
Cell Biology
Michael Way
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Luyan Cao, Foad Ghasemi, Michael Way, Antoine Jegou, Guillaume Romet-Lemonne
Summary: Activation of the Arp2/3 complex by actin nucleation-promoting factors leads to the formation of branching actin filaments, while direct nucleation of linear actin filaments is induced by SPIN90-Arp2/3. Interestingly, the VCA motifs of certain proteins destabilize existing branches and linear filament ends. However, branch stabilizer cortactin and destabilizer GMF have similar effects on SPIN90-activated Arp2/3. These findings highlight the importance of understanding the similarities and differences between the two mechanisms in regulating actin cytoskeleton dynamics, as well as the differential responses of branched and linear filaments to aging and mechanical stress.
Editorial Material
Cell Biology
Michael Way
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Miguel Hernandez-Gonzalez, Thomas Calcraft, Andrea Nans, Peter B. Rosenthal, Michael Way
Summary: A new pseudohexagonal lattice made of trimeric pillars forms inside immature vaccinia virus particles during maturation, leading to the transition from non-infectious spherical virions to infectious brick-shaped virions. The viral membrane becomes corrugated as it adapts to the newly formed viral core without membrane removal.
Article
Cell Biology
Elena Sindram, Andres Caballero-Oteyza, Naoko Kogata, Shaina Chor Mei Huang, Zahra Alizadeh, Laura Gamez-Diaz, Mohammad Reza Fazlollhi, Xiao Peng, Bodo Grimbacher, Michael Way, Michele Proietti
Summary: The Arp2/3 complex is essential for the formation of branched actin networks. We identified a frameshift variant in the ARPC5 gene in a child with recurrent infections and birth defects. Loss of ARPC5 affects actin cytoskeleton organization and leads to developmental defects in mice. ARPC5 should be considered as a candidate gene for early-onset immunodeficiency.
DISEASE MODELS & MECHANISMS
(2023)
Editorial Material
Cell Biology
Michael Way
JOURNAL OF CELL SCIENCE
(2023)