期刊
JOURNAL OF VIROLOGY
卷 86, 期 1, 页码 358-363出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.05460-11
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资金
- Australian Research Council (ARC) [LP0774913]
- Melbourne Water
- South East Water
- Australian Postgraduate Award
- La Trobe University
- Australian Research Council [LP0774913] Funding Source: Australian Research Council
Bacteriophages are considered to be the most abundant biological entities on the planet. The Siphoviridae are the most commonly encountered tailed phages and contain double-stranded DNA with an average genome size of similar to 50 kb. This paper describes the isolation from four different activated sludge plants of the phage RRH1, which is polyvalent, lysing five Rhodococcus species. It has a capsid diameter of only similar to 43 nm. Whole-genome sequencing of RRH1 revealed a novel circularly permuted DNA sequence (14,270 bp) carrying 20 putative open reading frames. The genome has a modular arrangement, as reported for those of most Siphoviridae phages, but appears to encode only structural proteins and carry a single lysis gene. All genes are transcribed in the same direction. RRH1 has the smallest genome yet of any described functional Siphoviridae phage. We demonstrate that lytic phage can be recovered from transforming naked DNA into its host bacterium, thus making it a potentially useful model for studying gene function in phages.
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