Article
Immunology
Anders J. Svendsen, Marie Christine Wulff Westergaard, Anette Holck Draborg, Rene Holst, Kirsten O. Kyvik, Marianne A. Jakobsen, Peter Junker, Gunnar Houen
Summary: The study revealed distinct differences in EBNA1 antibody levels between patients with RA and healthy individuals, as well as between relatives predisposed to RA and healthy subjects. The levels of EBNA1 antibodies associated with RA and family predisposition to RA are influenced by both genetics (including the shared epitope) and environmental factors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seifegebriel Teshome, Elshafa Hassan Ahmed, Kidist Zealiyas, Abdulaziz Abubeker, Fisihatsion Tadesse, Christoph Weigel, Robert A. Baiocchi, Tamrat Abebe
Summary: This study investigated the genetic variation of EBV in lymphoma patients in Ethiopia. The results showed a higher distribution of EBV genotype 1 in these patients, and a significant association between age groups and EBV genotypes was observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Yang Mei, Troy E. Messick, Jayaraju Dheekollu, Hee Jong Kim, Sudheer Molugu, Leonardo Josue Castro Munoz, Vera Moiskeenkova-Bell, Kenji Murakami, Paul M. Lieberman
Summary: EBV latent infection is associated with various diseases. EBNA1 is a viral protein that plays a crucial role in maintaining the virus during latent infection. In this study, the structure of EBNA1 DNA-binding domain (DBD) and its interaction with different sites were determined using cryo-EM. The N-terminal domain (NTD) of EBNA1 was also found to interact with specific DNA sequences. These findings provide insights into the mechanisms of EBNA1 binding and may have implications for targeting EBNA1 in EBV-associated diseases.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Seifegebriel Teshome, Kidist Zealiyas, Abdulaziz Abubeker, Fisihatsion Tadesse, Jayalakshmi Balakrishna, Christoph Weigel, Tamrat Abebe, Elshafa Hassan Ahmed, Robert A. Baiocchi
Summary: This study investigated the presence and viral load of Epstein-Barr virus (EBV) in lymphoma patients from Ethiopia using molecular and serological approaches. EBV DNA was detected in 99% of patients, and serological analyses showed EBV presence in 96% of cases. A high EBV viral load was observed in more than half of the patients.
Article
Virology
Kathy Shire, Edyta Marcon, Jack Greenblatt, Lori Frappier
Summary: Analysis of Epstein-Barr virus genomes revealed three amino acid changes in EBNA1 associated with gastric carcinoma and nasopharyngeal carcinoma. One of these mutations, Thr85Ala, was found to decrease transcriptional activation and have a gain of function interaction with PLOD1 and PLOD3, proteins linked to multiple cancers. Identifying the interaction between EBNA1 and PLOD1 provides new insights into the connection between EBV infection and cancer.
Article
Microbiology
Kidist Zealiyas, Seifegebriel Teshome, Aklilu Feleke Haile, Christoph Weigel, Ayinalem Alemu, Wondwossen Amogne, Getnet Yimer, Tamrat Abebe, Nega Berhe, Elshafa Hassan Ahmed, Robert A. Baiocchi
Summary: In Ethiopia, EBV genotype 1 was found to be the most predominant genotype, followed by EBV genotype 2. Understanding the genotype characterization of EBV in PWH is essential for developing new and innovative strategies for preventing and treating EBV-related complications in this population.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Wen Liu, Qianqian Zhang, Yan Zhang, Lingling Sun, Hua Xiao, Bing Luo
Summary: The relationship between Epstein-Barr virus (EBV) and gastric cancer is well-established, but the precise mechanisms of the virus in tumor formation remain unclear. This study found that the expression of endothelin-1 (ET-1) in EBV-associated gastric carcinoma (EBVaGC) was lower compared to Epstein-Barr virus-negative gastric carcinoma (EBVnGC), and it was associated with a lower frequency of lymph node metastasis in EBVaGC. Functional studies showed that the activation of the ET-1/endothelin receptor type A (ETAR) axis promoted cell growth, migration, and anti-apoptosis. The expression of ET-1 was not related to methylation of its promoter region or miRNAs. Treatment with a MEK1/2 inhibitor resulted in downregulation of ET-1 and forkhead box O1 (FOXO1) expression through the inactivation of the ERK1/2 pathway. Further experiments showed that FOXO1 knockdown decreased ET-1 expression. These findings suggest that ET-1 may play a role in the development of gastric cancer, and EBV suppresses its expression through the regulation of the transcription factor FOXO1 via the MAPK/ERK pathway.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Hua Xiao, Jingyang Sun, Weiqing Huang, Wen Liu, Yan Zhang, Haiyu Wang, Bing Luo
Summary: This study revealed that the expression of MAP9 is up-regulated in EBVaGC, and MAP9 plays a role in inhibiting the growth of EBVaGC cells. Additionally, the methylation of MAP9 CpG islands influences this regulation, with MAP9 acting as a tumor suppressor in the development of EBVaGC.
Article
Multidisciplinary Sciences
Munetoshi Hinata, Tetsuo Ushiku
Summary: A histology-based deep learning model was constructed to efficiently screen EBV and MSI/dMMR subtypes in gastric cancers, showing high accuracy in detecting the subgroup and highlighting intraepithelial lymphocytosis as a discriminative characteristic shared by EBV and MSI/dMMR tumors. This model offers a potential economical and less time-consuming alternative for stratifying patients who respond to ICIs.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Tomohiro Fujii, Jun Nishikawa, Soichiro Fukuda, Naoto Kubota, Junzo Nojima, Koichi Fujisawa, Ryo Ogawa, Atsushi Goto, Koichi Hamabe, Shinichi Hashimoto, Aung Phyo Wai, Hisashi Iizasa, Hironori Yoshiyama, Kohei Sakai, Yutaka Suehiro, Takahiro Yamasaki, Taro Takami
Summary: MC180295 inhibits the growth of EBVaGC cells by suppressing DNA repair and the cell cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Arghavan Zebardast, Sadra S. Tehrani, Tayebeh Latifi, Farzin Sadeghi
Summary: EBV-encoded miRNAs play a crucial role in the pathogenesis of EBVaGC, participating in carcinogenesis and the progression of malignancies by regulating gene expression and other activities.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Li-Jen Liao, Wan-Lun Hsu, Chi-Ju Chen, Yen-Ling Chiu
Summary: Nasopharyngeal carcinoma is endemic in southern Asia and this article describes the molecular abnormalities in NPC and potential molecular mechanisms for future therapy. It provides an up-to-date review on the molecular pathways of NPC, improving our understanding of the disease and highlighting areas for further research.
Article
Oncology
Liming Li, Wenpeng Huang, Ping Hou, Weiwei Li, Menyun Feng, Yiyang Liu, Jianbo Gao
Summary: This study aimed to develop a preoperative risk scoring model based on CT images to distinguish lymphoepithelioma-like gastric carcinoma (LELGC) from non-LELGC. The study found that enhancement pattern, CT-defined lymph node status, and attenuation in the venous phase were independent predictors of LELGC. The risk identification model showed high accuracy in both the training and validation cohorts.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Paul J. Farrell, Robert E. White
Summary: The incidence of diseases associated with EBV infection varies greatly in different parts of the world, which may be influenced by the variation in the virus genome. This article describes the main forms of EBV genome sequence variation and the potential contribution of these variations to disease development. Polymorphisms or deletions in the EBV genome can also serve as useful markers for disease monitoring. If certain strains of EBV are proven to be more pathogenic than others, immunization against these pathogenic strains may be valuable.
Review
Immunology
Ahmad Firoz, Hani Mohammed Ali, Suriya Rehman, Irfan A. Rather
Summary: Gastric cancer is a global health concern with a significant number of cases. Early diagnosis and treatment improve survival rates, but advanced stage tumors have lower survival rates. Oncolytic viruses show promise as more efficient diagnostic and treatment strategies. Oncogenic viruses play a significant role in gastric cancer progression.
Editorial Material
Oncology
Lawrence S. Young
CANCER COMMUNICATIONS
(2020)
Article
Public, Environmental & Occupational Health
Ashvin Kuri, Benjamin Meir Jacobs, Nicola Vickaryous, Julia Pakpoor, Jaap M. Middeldorp, Gavin Giovannoni, Ruth Dobson
Correction
Oncology
Anna E. Coghill, Ruth M. Pfeiffer, Carla Proietti, Wan-Lun Hsu, Yin-Chu Chien, Lea Lekieffre, Lutz Krause, Andy Teng, Jocelyn Pablo, Kelly J. Yu, Pei-Jen Lou, Cheng-Ping Wang, Zhiwei Liu, Chien-Jen Chen, Jaap Middeldorp, Jason Mulvenna, Jeff Bethony, Allan Hildesheim, Denise L. Doolan
CLINICAL CANCER RESEARCH
(2020)
Editorial Material
Microbiology
Lawrence S. Young
Article
Pathology
Jamie J. Beagan, Esther E. E. Drees, Phylicia Stathi, Paul P. Eijk, Laura Meulenbroeks, Floortje Kessler, Jaap M. Middeldorp, D. Michiel Pegtel, Josee M. Zijlstra, Daoud Sie, Danielle A. M. Heideman, Erik Thunnissen, Linda Smit, Daphne de Jong, Florent Mouliere, Bauke Ylstra, Margaretha G. M. Roemer, Erik van Dijk
Summary: This study demonstrates the feasibility of detecting somatic copy number alterations using PCR-free sWGS on cfDNA from plasma collected in EDTA or lithium-heparin tubes, paving the way for an automated cfDNA analysis workflow for samples from cancer patients. The percentage of unique reads was significantly higher in the PCR-free method compared to the PCR method, regardless of the type of collection tube, while other evaluated metrics showed high comparability between the two methods.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2021)
Article
Microbiology
Alexandra M. Davis, Abigail Rapley, Christopher W. Dawson, Lawrence S. Young, Mhairi A. Morris
Summary: The study demonstrates that LMP1 can recruit fibroblasts through an ERK-MAPK-dependent mechanism, enhancing their invasiveness and transformation to a myofibroblast-like phenotype, potentially leading to metastatic disease in NPC.
Article
Multidisciplinary Sciences
Insani Budiningsih, Yoes Prijatna Dachlan, Usman Hadi, Jaap Michiel Middeldorp
Summary: The study in Indonesia revealed that EBV infection and Plasmodium falciparum Malaria are risk factors in the development of Burkitt's lymphoma. There is a clear correlation between Malaria dysregulation and EBV infection, particularly in cases of P. falciparum.
Article
Immunology
Ute-Christiane Meier, Richard Christopher Cipian, Abbas Karimi, Ranjan Ramasamy, Jaap Michiel Middeldorp
Summary: Viral infections and aberrant immune responses play critical roles in driving localized neuroinflammation and neurodegeneration in multiple sclerosis (MS). Epstein-Barr virus (EBV) is considered to be a key player in MS pathogenesis, triggering localized inflammation near or within the central nervous system (CNS) through direct or indirect mechanisms involving B-cells, T-cells, cytokine-activated astrocytes and microglia cells damaging the myelin sheath of neurons. Genetic factors like HLA-DR2b may contribute to aberrant EBV antigen-presentation and autoimmune responses characteristic of MS.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pediatrics
Marlinda Adham, Namira Kesuma Jelita, Djajadiman Gatot, Soehartati Argadikoesoema Gondhowiardjo, Lisnawati Rachmadi, Astrid E. Greijer, I. Bing Tan, Jaap M. Middeldorp
Summary: Studies in a national referral hospital in Indonesia found that higher EBV DNA load in pediatric NPC patients is associated with advanced tumor stages, but not necessarily with treatment response as evaluated by CT scan.
PAEDIATRICA INDONESIANA
(2021)
Article
Medical Laboratory Technology
Geng-Hang Chen, Zhiwei Liu, Kelly J. Yu, Anna E. Coghill, Xiao-Xia Chen, Shang-Hang Xie, Dong-Feng Lin, Qi-Hong Huang, Yu-Qiang Lu, Wei Ling, Chu-Yang Lin, Zi-Jian Lu, Yu-Ying Fan, Lin-Quan Tang, Joshua N. Sampson, Hui Li, Ann D. King, Jaap M. Middeldorp, Allan Hildesheim, Su-Mei Cao
Summary: This study evaluated the performance of nasopharynx swab EBV DNA as a reflex test to triage high-risk individuals for nasopharyngeal carcinoma (NPC). The results showed that the swab test had promising potential in reducing referral rate without significantly compromising sensitivity compared to serology-only screening programs.
CLINICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Michelle H. F. Dias, Luiz F. F. Guimaraes, Matheus G. Barcelos, Eduardo U. M. Moreira, Maria F. A. do Nascimento, Tais N. de Souza, Camilla Pires, Talita A. F. Monteiro, Jaap M. Middeldorp, Irene S. Soares, Cor J. F. Fontes, Francis B. Ntumngia, John H. Adams, Flora S. Kano, Luzia H. Carvalho
Summary: Simultaneous infection of Plasmodium falciparum and Epstein-Barr virus (EBV) can promote the development of Burkitt's Lymphoma, and EBV infection may impact human immune response to malaria. This study investigated the impact of EBV co-infection on antibody responses to P. vivax in a population exposed to malaria.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Microbiology
Christina B. Karsten, Yannic C. Bartsch, Sally A. Shin, Matthew D. Slein, Howard M. Heller, Kumaran Kolandaivelu, Jaap M. Middeldorp, Galit Alter, Boris Julg
Summary: Although Epstein-Barr virus primarily causes asymptomatic infections, it can also lead to associated diseases. However, the antibody function during EBV infection is relatively limited compared to other viral infections, possibly due to the virus transitioning from lytic to latent stages.
Review
Oncology
Lee Fah Yap, Anna Kang Chee Wong, Ian C. Paterson, Lawrence S. Young
Summary: Epstein-Barr virus (EBV) is a human tumor virus that plays a critical role in the development of various cancers. It has two life cycles, latent and lytic, with latent genes driving disease development and lytic genes responsible for viral transmission. Recent studies have shown that EBV lytic genes are expressed in tumor tissues and cell lines, suggesting their significant involvement in tumorigenesis.
Article
Oncology
Ilona Argirion, Ruth M. Pfeiffer, Carla Proietti, Anna E. Coghill, Kelly J. Yu, Jaap M. Middeldorp, Yomani D. Sarathkumara, Wan-Lun Hsu, Yin-Chu Chien, Pei-Jen Lou, Cheng-Ping Wang, Nathaniel Rothman, Qing Lan, Chien-Jen Chen, Sam M. Mbulaiteye, Ruth F. Jarrett, Ingrid Glimelius, Karin E. Smedby, Henrik Hjalgrim, Allan Hildesheim, Denise L. Doolan, Zhiwei Liu
Summary: By studying the anti-EBV IgG and IgA antibody responses in patients with four different EBV-associated cancers, it was found that the antibody responses were associated with different cancer types. This research helps to understand the pathogenic mechanisms of EBV-related cancers.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2023)
Correction
Multidisciplinary Sciences
Lijun Jiang, Hong Lok Lung, Tao Huang, Rongfeng Lan, Shuai Zha, Lai Sheung Chan, Waygen Thor, Tik-Hung Tsoi, Ho-Fai Chau, Cecilia Borestrom, Steven L. Cobb, Sai Wah Tsao, Zhao-Xiang Bian, Ga-Lai Law, Wing-Tak Wong, William Chi-Shing Tai, Wai Yin Chau, Yujun Du, Lucas Hao Xi Tang, Alan Kwok Shing Chiang, Jaap M. Middeldorp, Kwok-Wai Lo, Nai Ki Mak, Nicholas J. Long, Ka-Leung Wong
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)