Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Jie Wang, Kun-Te Shang, Qiong-Hong Ma, Zhao-Ying Dong, Yi-Hong Chen, Yu-Feng Yao
Summary: This study aimed to determine whether HSV-1 can be transmitted through TNTs and to investigate the effect of inhibiting the Arp2/3 complex on the intercellular transmission of HSV-1. The results showed that HSV-1 can be transmitted through TNTs and that inhibiting the Arp2/3 complex reduces the number of TNTs and the spread of HSV-1. This finding provides new insights into the transmission mode of HSV-1 and suggests a potential new antiviral target.
Article
Virology
Krista G. Freeman, Jamie B. Huffman, Fred L. Homa, Alex Evilevitch
Summary: Viruses undergo a maturation process to meet conflicting virion assembly requirements. Herpesviruses achieve capsid reinforcement and stable DNA encapsidation through a mechanical capsid maturation process facilitated by multifunctional auxiliary protein UL25. Gradual capsid reinforcement by progressive UL25 binding is a feasible mechanism correlated with DNA packaging progression, presenting a new antiviral strategy resistant to drug resistance.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Xueqi Li, Jianbin Wang, Tangwei Mou, Yang Gao, Lichun Wang, Shengtao Fan, Xingli Xu, Guorun Jiang, Pingfang Cui, Xiangxiong Xu, Suqin Duan, Jingjing Zhang, Dandan Li, Yun Liao, Li Yu, Heng Zhao, Ming Lu, Hailian Zhu, Ran Gu, Ying Zhang, Wei Dong, Qihan Li
Summary: Convalescent serum from HSV2-infected patients predominantly recognized the ICP35 protein encoded by gene UL26.5, which could be used to design an integrated antigen with other viral glycoproteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Mizuki Watanabe, Jun Arii, Kosuke Takeshima, Ayano Fukui, Masayuki Shimojima, Hiroko Kozuka-Hata, Masaaki Oyama, Takeharu Minamitani, Teruhito Yasui, Yuji Kubota, Mutsuhiro Takekawa, Isao Kosugi, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi
Summary: The host cell protein prohibitin-1 contributes to HSV-1 cell-to-cell spread via the MAPK/ERK pathway, a mechanism that is conserved in representative members of every herpesvirus subfamily.
JOURNAL OF VIROLOGY
(2021)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eduardo I. Tognarelli, Luisa F. Duarte, Monica A. Farias, Felipe A. Cancino, Nicolas Corrales, Francisco J. Ibanez, Claudia A. Riedel, Susan M. Bueno, Alexis M. Kalergis, Pablo A. Gonzalez
Summary: Stimulating HO-1 expression in HSV-infected dendritic cells can promote cell viability and inhibit viral egress. It also promotes the expression of anti-inflammatory molecules and activation of virus-specific CD4(+) T cells. Transferring HSV-infected dendritic cells with induced HO-1 expression can enhance virus-specific T cell activation and improve the outcome of HSV-1 skin infection.
Article
Chemistry, Applied
Renjie Li, Pernille Lund, Soren B. Nielsen, Marianne N. Lund
Summary: This study aimed to generate soluble aggregates of whey protein isolates at lower temperature through partial hydrolysis before thermal treatment. The partial hydrolysis induced structural changes and exposure of free thiol groups in the proteins, resulting in soluble protein aggregates with a particle size range of 10-100 nm in radius for BLP treatment and 7-50 nm for TP treatment. Additionally, disulfide bonds were found to contribute to the association of protein aggregates, but the aggregates obtained were not stable under UHT conditions.
FOOD HYDROCOLLOIDS
(2022)
Article
Immunology
Malgorzata Krzyzowska, Anders Jarneborn, Karolina Thorn, Kristina Eriksson, Tao Jin
Summary: This study provides evidence that treatment with tofacitinib may increase the risk of disease aggravation and severe encephalitis in primary herpes simplex infection by impairing antiviral response induced by monocytes and microglia.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Virology
Bangxing Hong, Upasana Sahu, Matthew P. Mullarkey, Balveen Kaur
Summary: Oncolytic herpes simplex virus (oHSV) shows promise as a treatment for solid tumors, but there are still barriers that limit its efficacy. This review focuses on the essential viral genes that allow for virus replication and spread, with the aim of enhancing oHSV infection and replication among tumor cells.
Article
Microbiology
Kevin Danastas, Gerry Guo, Jessica Merjane, Nathan Hong, Ava Larsen, Monica Miranda-Saksena, Anthony L. Cunningham
Summary: This study explores the effects of interferons (IFNs) on herpes simplex virus-1 (HSV-1) and reveals that IFNs have the potential to block virus release from nerve endings, thereby preventing transmission into the skin. The study also highlights the potential wider antiviral effects of IFN-γ in neurons, suggesting its role in HSV-1 reactivation. These findings identify new targets for the development of immunotherapies to impede HSV-1 spread from nerves to the skin.
Article
Biology
Manutea C. Serrero, Virginie Girault, Sebastian Weigang, Todd M. Greco, Ana Ramos-Nascimento, Fenja Anderson, Antonio Piras, Ana Hickford Martinez, Jonny Hertzog, Anne Binz, Anja Pohlmann, Ute Prank, Jan Rehwinkel, Rudolf Bauerfeind, Ileana M. Cristea, Andreas Pichlmair, Georg Kochs, Beate Sodeik, Adam P. Geballe
Summary: This study identifies an interferon-inducible protein, MxB, that can sense herpesviral capsids and disassemble them, thereby restricting viral nuclear targeting and progeny capsid assembly, leading to enhanced innate immune responses.
Article
Medicine, Research & Experimental
Catielen Paula Pavi, Isabella Dai Pra, Rafael Dorighello Cadamuro, Isamu Kanzaki, Jhuly Wellen Ferreira Lacerda, Louis Pergaud Sandjo, Roberto Messias Bezerra, Jorge Federico Orellana Segovia, Gislaine Fongaro, Izabella Thais Silva
Summary: This study evaluated the activity of seventeen extracts derived from eight Amazonian medicinal plants against herpes simplex virus and chikungunya virus. Four extracts exhibited significant activity against both viruses, including the drug-resistant strains. Virucidal tests showed a higher level of virucidal activity compared to antiviral activity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Virology
Mashidur Rana, Mizumi Setia, Pratima K. Suvas, Anish Chakraborty, Susmit Suvas
Summary: Reactive oxygen species (ROS) play a crucial role in herpes stromal keratitis (HSK), with neutrophils being the primary cells responsible for ROS generation. NOX2 is involved in ROS production in neutrophils. However, manipulating ROS levels in neutrophils may help alleviate the severity of HSK, and NOX2 is not the main contributor to ROS generation in neutrophils.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Savithri Weerasooriya, Katherine A. DiScipio, Anthar S. Darwish, Ping Bai, Sandra K. Weller
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Yudong Wang, Johan Palmfeldt, Niels Gregersen, Alexander M. Makhov, James F. Conway, Meicheng Wang, Stephen P. McCalley, Shrabani Basu, Hana Alharbi, Claudette St Croix, Michael J. Calderon, Simon Watkins, Jerry Vockley
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Virology
Mitali Adlakha, Christine M. Livingston, Irina Bezsonova, Sandra K. Weller
JOURNAL OF VIROLOGY
(2020)
Article
Multidisciplinary Sciences
Fengbin Wang, Ying Liu, Zhangli Su, Tomasz Osinski, Guilherme A. P. de Oliveira, James F. Conway, Stefan Schouten, Mart Krupovic, David Prangishvili, Edward H. Egelman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Alexis Huet, Robert L. Duda, Pascale Boulanger, James F. Conway
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biotechnology & Applied Microbiology
Willie Taylor, Emily Camilleri, D. Levi Craft, George Korza, Maria Rocha Granados, Jaliyah Peterson, Renata Szczpaniak, Sandra K. Weller, Ralf Moeller, Thierry Douki, Wendy W. K. Mok, Peter Setlow
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Cell Biology
Ximena Corso-Diaz, James Gentry, Ryan Rebernick, Catherine Jaeger, Matthew J. Brooks, Freekje van Asten, Keshav Kooragayala, Linn Gieser, Jacob Nellissery, Raul Covian, Tiziana Cogliati, Anupam K. Mondal, Ke Jiang, Anand Swaroop
Article
Virology
Alexis Huet, Jamie B. Huffman, James F. Conway, Fred L. Homa
JOURNAL OF VIROLOGY
(2020)
Article
Genetics & Heredity
Passley Hargrove-Grimes, Anupam K. Mondal, Jessica Gumerson, Jacob Nellissery, Angel M. Aponte, Linn Gieser, Haohua Qian, Robert N. Fariss, Juan S. Bonifacino, Tiansen Li, Anand Swaroop
Editorial Material
Biochemistry & Molecular Biology
Sandra K. Weller, Neal A. Deluca
Summary: The dynamic interactions between invading viral pathogens and their hosts, particularly the ability of herpes simplex virus (HSV) to establish life-long infections in humans, are governed by a balance between maintaining a latent state and re-entering the lytic phase during reactivation. This balance is influenced by innate immune signaling, intrinsic cellular antiviral mechanisms, and epigenetic repression of the viral genome. The study by Suzich et al. (2021) provides a comprehensive model that integrates individual observations and sheds light on the mechanisms underlying viral genome repression and latency.
Article
Immunology
Matthew D. Hall, James M. Anderson, Annaliesa Anderson, David Baker, Jay Bradner, Kyle R. Brimacombe, Elizabeth A. Campbell, Kizzmekia S. Corbett, Kara Carter, Sara Cherry, Lillian Chiang, Tomas Cihlar, Emmie de Wit, Mark Denison, Matthew Disney, Courtney Fletcher, Stephanie L. Ford-Scheimer, Matthias Gotte, Abigail C. Grossman, Frederick G. Hayden, Daria J. Hazuda, Charlotte A. Lanteri, Hilary Marston, Andrew D. Mesecar, Stephanie Moore, Jennifer O. Nwankwo, Jules O'Rear, George Painter, Kumar Singh Saikatendu, Celia A. Schiffer, Timothy P. Sheahan, Pei-Yong Shi, Hugh D. Smyth, Michael J. Sofia, Marla Weetall, Sandra K. Weller, Richard Whitley, Anthony S. Fauci, Christopher P. Austin, Francis S. Collins, Anthony J. Conley, Mindy Davis
Summary: The NIH Virtual SARS-CoV-2 Antiviral Summit aimed to provide an overview of the status and challenges in developing antiviral therapeutics for COVID-19, with the goals of reviewing the current science, sharing insights, and identifying opportunities for collaboration.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Daniel J. Goetschius, Samantha R. Hartmann, Lindsey J. Organtini, Heather Callaway, Kai Huang, Carol M. Bator, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Colin R. Parrish, Susan L. Hafenstein
Summary: The study identified a significant overlap between the antigenic epitope and the receptor binding site of canine parvovirus, showing that specific antibody binding induces conformational changes to the virus. The findings highlight the potential of using cryo-EM to investigate complementarity of antibody binding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Katherine A. DiScipio, Savithri Weerasooriya, Renata Szczepaniak, Akram Hazeen, Lee R. Wright, Dennis L. Wright, Sandra K. Weller
Summary: Most drug discovery efforts against herpesviruses have focused on nucleoside analogs, but this study proposes a new strategy targeting two-metal ion-dependent viral enzymes. The results showed weak activity of integrase inhibitors and inhibition of HSV-1 and CMV by 8-hydroxyquinolines. The compounds exhibited antiviral activity through different mechanisms, providing an opportunity for the development of dual-targeting agents against herpesviruses.
Article
Biochemistry & Molecular Biology
Herman K. H. Fung, Shelley Grimes, Alexis Huet, Robert L. Duda, Maria Chechik, Joseph Gault, Carol Robinson, Roger W. Hendrix, Paul J. Jardine, James F. Conway, Christoph G. Baumann, Alfred A. Antson
Summary: Many cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. Researchers have reconstituted a cos packaging system and provided a detailed description of its biochemical and structural properties, revealing similarities and differences in its mechanism and regulation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Katherine A. DiScipio, Savithri Weerasooriya, Renata Szczepaniak, Akram Hazeen, Lee R. Wright, Dennis L. Wright, Sandra K. Weller
Summary: The research focused on targeting viral enzymes based on two-metal ion-dependent strategy. It was found that integrase inhibitors exhibited weak activity against HSV-1, while 8-hydroxyquinolines could inhibit both HSV-1 and cytomegalovirus, providing an opportunity for the development of dual-targeting agents against herpesviruses.