期刊
JOURNAL OF VIROLOGY
卷 85, 期 16, 页码 8449-8452出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.05054-11
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Founding Research Centers for Emerging and Reemerging Infectious Diseases
- ERATO (Japan Science and Technology Agency)
- National Institute of Allergy and Infectious Diseases Public Health Service
- National Institute of Allergy and Infectious Diseases [HHSN266200700010C]
- Grants-in-Aid for Scientific Research [21580372] Funding Source: KAKEN
Reassortment is important for influenza virus evolution and the generation of novel viruses with pandemic potential; however, the factors influencing reassortment are still poorly understood. Here, using reverse genetics and a replicon assay, we demonstrated that a mixed polymerase complex containing a pandemic (H1N1) 2009 influenza virus PB2 on a seasonal H1N1 virus background has reduced polymerase activity, leading to impaired virus viability. Adaptation of viruses containing the mixed polymerase complex resulted in compensatory mutations in PB1. Taken together, our results identify the cooperation between PB2 and PB1 as an important restricting factor for reassortment of influenza viruses.
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