期刊
JOURNAL OF VIROLOGY
卷 84, 期 18, 页码 9642-9648出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00326-10
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资金
- National Institute of Allergy and Infectious Diseases [AI-051560]
Previously, we showed that overexpression of MIP-1 alpha in mouse brain further decreased rabies virus (RABV) pathogenicity (L. Zhao, H. Toriumi, Y. Kuang, H. Chen, and Z. F. Fu, J. Virol., 83: 11808-11818, 2009). In the present study, the immunogenicity of recombinant RABV expressing MIP-1 alpha (rHEP-MIP1 alpha) was determined. It was found that intramuscular immunization of BALB/c mice with rHEP-MIP1 alpha resulted in a higher level of expression of MIP-1 alpha at the site of inoculation, increased recruitment of dendritic cells (DCs) and mature B cells into the draining lymph nodes and the peripheral blood, and higher virus-neutralizing antibody titers than immunization with the parent rHEP and recombinant RABVs expressing RANTES (CCL5) or IP-10 (CXCL10). Our data thus demonstrate that expression of MIP-1 alpha not only reduces viral pathogenicity but also enhances immunogenicity by recruiting DCs and B cells to the site of immunization, the lymph nodes, and the blood.
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