Article
Virology
Craig Schindewolf, Kumari Lokugamage, Michelle N. Vu, Bryan A. Johnson, Dionna Scharton, Jessica A. Plante, Birte Kalveram, Patricia A. Crocquet-Valdes, Stephanea Sotcheff, Elizabeth Jaworski, Rojelio E. Alvarado, Kari Debbink, Matthew D. Daugherty, Scott C. Weaver, Andrew L. Routh, David H. Walker, Kenneth S. Plante, Vineet D. Menachery
Summary: Understanding the role of NSP16 in SARS-CoV-2 infection and pathogenesis, this study demonstrates the attenuation of a NSP16 mutant both in vitro and in vivo, as well as its sensitivity to IFN-I. Silencing IFIT1 or IFIT3 partially restores the mutant's fitness. Furthermore, targeting NSP16 with a MTase inhibitor enhances the virus' sensitivity to IFN-I. Overall, these findings highlight the importance of NSP16 in immune evasion and suggest a potential target for antiviral development.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
Lea Meyer, Isoline Duquenois, Stacy Gellenoncourt, Marie Pellerin, Ailona Marcadet-Hauss, Nicole Pavio, Virginie Doceul
Summary: Hepatitis E virus (HEV) is a common cause of enterically transmitted acute hepatitis worldwide. Swine are the main reservoir of zoonotic HEV and can control the virus pathogenesis. Interferon-stimulated genes (ISGs) can restrict the replication of HEV.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Hua Yin, Shaowei Li, Chunxia Chai, Fan Zhang, Yanhua Ma, Youzhi Wu, Cun Fu, Yun Diao, Yanyan Zhou, Jinlong Zhang, Rui Niu, Wei Wang
Summary: In this study, a recombinant expression plasmid carrying bovine interferon a (BoIFN-a) gene was successfully constructed and expressed in E. coli BL21 (DE3) cells. The expressed recombinant BoIFN-a protein (rBoIFN-a) was about 36KD and present as inclusion bodies. Stimulation of MDBK cells with denatured, purified, and renatured rBoIFN-a protein up-regulated the expression of interferon-stimulated genes (ISGs) and effectively inhibited the replication of BVDV in vitro.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Sheng Wang, Lijun Wan, Hongyu Ren, Zhixun Xie, Liji Xie, Jiaoling Huang, Xianwen Deng, Zhiqin Xie, Sisi Luo, Meng Li, Tingting Zeng, Yanfang Zhang, Minxiu Zhang
Summary: Avian reovirus (ARV) infection can cause immunosuppression and pathological damage. The study highlights the role of interferon and interferon-stimulated genes (ISGs) in the early stage of infection, with IFIT5 playing a crucial role in inhibiting ARV replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Hailing Zhang, Dongliang Zhang, Shasha Zhang, Hao Liu, Han Wang, Cong Wang, Deying Zou, Bo Hu, Shizhen Lian, Shiying Lu, Xue Bai
Summary: The interferon (IFN) response is the first line of defense against viral invasion and plays a central role in immune response regulation. IFN-epsilon, a newly discovered type I IFN, is constitutively expressed in epithelial cells and has important functions in mucosal immunity. This study evaluated the biological activity of the mink-IFN (MiIFN)-epsilon gene and its antiviral role against vesicular stomatitis virus (VSV) in epithelial cells.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Immunology
Dan Zhang, Aaron T. Irving
Summary: The interferon pathway is the first line of defense against viral infections in mammals, and it induces the expression of interferon-stimulated genes (ISGs). The antiviral function of ISGs has been extensively studied in mice and humans. Bats, as an important viral reservoir, can coexist with pathogenic viruses without showing signs of disease, but limited data are available on the role of ISGs in bats. This review summarizes the current understanding of conserved and bat-specific ISGs and their known antiviral functions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Hailing Zhang, Dongliang Zhang, Han Lu, Deying Zou, Bo Hu, Shizhen Lian, Shiying Lu
Summary: This study synthesized the gene for mature mink interferon alpha and constructed a recombinant plasmid, providing a foundation for large-scale production of recombinant interferons. The purified MilFN-alpha exhibited significant antiviral activity against various viruses, effectively inhibiting their replication.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Mengjia Shen, Congcong Duan, Changhao Xie, Hongtao Wang, Zhijun Li, Baiqing Li, Tao Wang
Summary: This study identified differentially expressed ISGs in the PBMCs of SLE patients and healthy individuals, including NRIR, RSAD2, USP18, IFI44, and ISG15. These findings may contribute to future diagnosis and new personalized targeted therapies for SLE.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Fan-Xiang Gao, Wei-Jia Lu, Yan Shi, Zhan-Wei Zhao, Li Zhou, Jian-Fang Gui, Zhe Zhao
Summary: This study systematically analyzed IFN genes in pufferfish and identified different types of IFN genes, studying their molecular characteristics and phylogenetic relationships. The results showed that these IFN genes have different expression patterns in various tissues of pufferfish and play multiple roles in antiviral responses. Additionally, specific transcription factor binding sites and induction effects were investigated.
AQUACULTURE REPORTS
(2022)
Article
Plant Sciences
Weili Liu, Weichao Ren, Xiubo Liu, Lianqing He, Chen Qin, Panpan Wang, Lingyang Kong, Yang Li, Yunwei Liu, Wei Ma
Summary: This study conducted a whole-genome analysis of Chinese dwarf cherry and identified 25 Dof genes, revealing their structure, characteristics, chromosomal distribution, and properties of the encoded proteins. Transcriptome data showed tissue-specific expression of most Dof genes. Analysis of gene expression patterns during fruit storage revealed their important role in this process.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Gastroenterology & Hepatology
Lena Allweiss, Katja Giersch, Andrea Pirosu, Tassilo Volz, Robert C. Muench, Rudolf K. Beran, Stephan Urban, Hassan Javanbakht, Simon P. Fletcher, Marc Luetgehetmann, Maura Dandri
Summary: Therapeutic strategies targeting HBx, such as siRNA and peg-IFN alpha, can reduce HBV markers and facilitate the reappearance of SMC5/6 in hepatocytes achieving HBV-RNA negativity and association with cccDNA. However, the antiviral effects are not sustained off treatment, leading to degradation of SMC5/6.
Article
Biochemistry & Molecular Biology
Zishan Feng, Xiaohua Wu, Jian Wang, Xinyi Wu, Baogen Wang, Zhongfu Lu, Zihong Ye, Guojing Li, Ying Wang
Summary: The OVATE gene family plays significant roles in plant growth, development, and abiotic stress, as well as fruit shape in vegetable crops. In this study, we identified and analyzed the OVATE gene family in bottle gourd, and found that the LsOVATE1 gene could serve important roles in bottle gourd development and fruit shape determination.
Article
Plant Sciences
Xiaojiao Yu, Shengfu Zhong, Huai Yang, Chen Chen, Wei Chen, Hao Yang, Ju Guan, Peng Fu, Feiquan Tan, Tianheng Ren, Jinliang Shen, Min Zhang, Peigao Luo
Summary: Akebia trifoliata, an important multiuse perennial plant, lacks research on its resistance genes, hindering the breeding of resistant varieties. Genes encoding proteins with NBS and LRR motifs are crucial for plant disease resistance, and a comprehensive genome-wide analysis identified 73 NBS genes in the A. trifoliata genome.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Genetics & Heredity
Weifeng Zhang, Fei Yin, Yongbo Bao
Summary: This study systematically investigated the distribution of bHLH genes in nine molluscs and classified them into corresponding families. The results showed genetic conservation of bHLH transcription factors during evolution, with low similarity in group C. Transcription profile analysis revealed the important role of bHLH genes in responding to external challenges, development, and tissue-specific expression.
Article
Multidisciplinary Sciences
Armando Garces, Bryan Martinez, Roberto de la Garza, Deepa Roy, Kaylie-Anna Vallee, Jerel Adam Fields, David J. Moore, Hansapani Rodrigo, Upal Roy
Summary: The United Nations predicts that by 2050, one in every six people will be over the age of 65. With an increasing elderly population, the risk of Alzheimer's disease (AD) is a significant concern. AD is a multifactorial disease that involves neurodegeneration, mild dementia, and cognitive deficits. Additionally, individuals with HIV-1 experience accelerated aging and an increased risk of developing HIV-associated neurocognitive disorders (HAND). This study focuses on identifying differentially expressed genes in both AD and HAND, and the results highlight the role of interferon pathways in the pathologies of these diseases within the brain, providing insights for future detection.
SCIENTIFIC REPORTS
(2023)