Article
Biophysics
Annett Petrich, Valentin Dunsing, Sara Bobone, Salvatore Chiantia
Summary: The study found that M2 recruits M1 to the plasma membrane through a strong interaction between the two proteins, while there is only a weak interaction between M1 and HA, which occurs only when M1 is already bound to the plasma membrane.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jitendriya Swain, Maxime Bierre, Laura Veyrie, Charles-Adrien Richard, Jean-Francois Eleouet, Delphine Muriaux, Monika Bajorek
Summary: Human respiratory syncytial virus (RSV) is a major cause of infantile bronchiolitis and childhood deaths. Research has found that the virus's M protein can cluster on the plasma membrane by selectively binding with phosphatidylserine (PS), forming filamentous infectious viral particles. Other negatively charged lipids do not enhance M binding, and cholesterol negatively affects M interaction with membrane lipids.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Vladislav V. Khrustalev, Larisa V. Kordyukova, Alexander M. Arutyunyan, Victor V. Poboinev, Tatyana A. Khrustaleva, Aleksander N. Stojarov, Lyudmila A. Baratova, Alena S. Sapon, Valery G. Lugin
Summary: This study reveals that the cytoplasmic tail (CT) of the influenza A/H1N1 virus hemagglutinin (HA) may form a short antiparallel beta-structure under the lipid membrane within the virion, which is crucial for virus pathogenesis.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Virology
Danielle Thompson, Christiana Victoria Cismaru, Jean-Sebastien Rougier, Martin Schwemmle, Gert Zimmer
Summary: The proton channel activity of bat influenza A virus (IAV) M2 proteins was found to be low, indicating that they are unable to fully protect acid-sensitive HA protein from conformational changes. The primary sequences of bat IAV M2 proteins also differ significantly from conventional IAV M2 proteins, suggesting possible differences in their biological function. This study reveals the importance of the proton channel activity of bat IAV M2 proteins in virus replication.
JOURNAL OF VIROLOGY
(2023)
Article
Chemistry, Multidisciplinary
Alessandro Piai, Qingshan Fu, Amanda K. Sharp, Beatrice Bighi, Anne M. Brown, James J. Chou
Summary: This study determined the structure of the cytoplasmic tail (CT) of HIV-1 envelope glycoprotein through NMR, and derived a model of the membrane-interacting region, showcasing stability in a viral membrane and compensation for lipid exclusion by the KS-LLP2 region through molecular dynamics simulations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Immunology
Yu Zhang, Yuan Yuan, Li-Hua Zhang, Dan Zhu, Lu Wang, Lan-Ping Wei, Wen-Sheng Fan, Chang-Run Zhao, Yan-Jing Su, Jian-Qi Liao, Lu Yong, Tian-Chao Wei, Ping Wei, Mei-Lan Mo
Summary: This study evaluated virus-like particles (VLPs) carrying S, M, and E proteins from a dominant IBV strain in China. The results demonstrated that SME-VLPs induced strong humoral, cellular, and mucosal immune responses, offering effective protection against IBV.
Article
Chemistry, Physical
Michal Michalski, Piotr Setny
Summary: Influenza virus enters host cells through membrane fusion, which is facilitated by the viral hemagglutinin protein (HA). Our computational study reveals the important role of the HA C-terminal domain in fusion, shedding light on its structural and functional interdependencies.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biology
Martin D. Gelenter, Venkata S. Mandala, Michiel J. M. Niesen, Dina A. Sharon, Aurelio J. Dregni, Adam P. Willard, Mei Hong
Summary: By using solid-state NMR spectroscopy and molecular dynamics simulations, researchers found that water molecules in the channel of the influenza B M2 protein exhibit faster dynamics and higher orientational order in the open state compared to the closed state, establishing the necessary water network structure for proton hopping.
COMMUNICATIONS BIOLOGY
(2021)
Article
Virology
Hae-Ji Kang, Ki-Back Chu, Keon-Woong Yoon, Gi-Deok Eom, Jie Mao, Min-Ju Kim, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Summary: Research suggests that avian influenza VLP vaccines expressing multiple neuraminidases can provide both homologous and heterosubtypic protection against different subtypes, offering a promising approach for developing a universal influenza A vaccine against avian and human influenza virus infections.
Review
Microbiology
Alison Simancas-Racines, Santiago Cadena-Ullauri, Patricia Guevara-Ramirez, Ana Karina Zambrano, Daniel Simancas-Racines
Summary: Avian influenza is a contagious disease that causes high avian mortality, leading to economic losses and increased costs for disease control. It is caused by an RNA virus and only Influenzavirus A can infect birds. The pathogenicity of avian influenza is based on the lethality, signs, and molecular characteristics of the virus. Low pathogenic avian influenza has a low mortality rate, while highly pathogenic avian influenza can cross barriers and damage all tissues with a high mortality rate. The zoonotic potential of avian influenza has made it a global public health concern.
Article
Biochemistry & Molecular Biology
Tisza A. S. Bell, Nileena Velappan, Cheryl D. Gleasner, Gang Xie, Shawn R. Starkenburg, Geoffrey Waldo, Shounak Banerjee, Sofiya N. Micheva-Viteva
Summary: This study found that the activation profiles of autophagy regulating genes differ during influenza A virus (IAV) infection at different multiplicities of infection and in different cell types, and that JNK-Thr187 signaling is crucial for IAV replication.
MOLECULAR MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Yuchong Wang, Xujun Zhang, Kefan Bi, Hongyan Diao
Summary: MicroRNAs play important roles in regulating virus replication and host antiviral response during H1N1 infection, with some defending against the virus and others promoting viral replication. They are involved in host-viral interactions and have versatile functions in this process.
Article
Virology
Rashid Manzoor, Nao Eguchi, Reiko Yoshida, Hiroichi Ozaki, Tatsunari Kondoh, Kosuke Okuya, Hiroko Miyamoto, Ayato Takada
Summary: Protective immunity against influenza A viruses mainly relies on antibodies to hemagglutinin (HA), but the conserved M2 protein has been studied as a vaccine antigen for cross-protective immunity. This study reveals that an M2-specific antibody can interfere with the HA-M2 association, providing a novel mechanism for the antiviral activity of M2-specific antibodies.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Ujjwal Kumar, Priya Goyal, Zaid K. Madni, Kajal Kamble, Vineet Gaur, Maitreyi S. Rajala, Dinakar M. Salunke
Summary: This study obtained four unique single-chain antibodies against M2e through screening human antibody libraries, which can bind to the conserved site of M2e and the full-length M2 protein expressed on HEK293T cells. The structural and biochemical study showed the binding of these antibodies with M2e peptide dimer, providing insights into their function of recognizing and restricting the release of new viral particles from an infected host cell.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Immunology
Vasilis C. Pliasas, Zach Menne, Virginia Aida, Ji-Hang Yin, Maria C. Naskou, Peter J. Neasham, J. Fletcher North, Dylan Wilson, Katharine A. Horzmann, Joshy Jacob, Ioanna Skountzou, Constantinos S. Kyriakis
Summary: This study evaluated the immunogenicity and efficacy of a novel vaccine against influenza virus infection in a pig model. The results showed that the vaccine induced high levels of anti-NA antibodies and provided protection comparable to a commercial vaccine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Physiology
Jackye Peretz, Andrew Pekosz, Andrew P. Lane, Sabra L. Klein
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2016)
Article
Obstetrics & Gynecology
Shriddha Nayak, Jun Lei, Andrew Pekosz, Sabra Klein, Irina Burd
SEMINARS IN REPRODUCTIVE MEDICINE
(2016)
Article
Public, Environmental & Occupational Health
J. H. Leibler, K. Dalton, A. Pekosz, G. C. Gray, E. K. Silbergeld
ZOONOSES AND PUBLIC HEALTH
(2017)
Article
Virology
Leigh M. Howard, Monika Johnson, Ana I. Gil, Andrew Pekosz, Marie R. Griffin, Kathryn M. Edwards, Claudio F. Lanata, Carlos G. Grijalva, John V. Williams
JOURNAL OF CLINICAL VIROLOGY
(2017)
Article
Virology
Hsuan Liu, Michael L. Grantham, Andrew Pekosz
JOURNAL OF VIROLOGY
(2018)
Article
Immunology
Nicholas Wohigemuth, Yang Ye, Katherine J. Fenstermacher, Hsuan Liu, Andrew P. Lane, Andrew Pekosz
Article
Immunology
Adriana Forero, Katherine Fenstermacher, Nicholas Wohlgemuth, Andrew Nishida, Victoria Carter, Elise A. Smith, Xinxia Peng, Melissa Hayes, Doreen Francis, John Treanor, Juliet Morrison, Sabra L. Klein, Andrew Lane, Michael G. Katze, Andrew Pekosz
Article
Multidisciplinary Sciences
Meghan S. Vermillion, Jun Lei, Yahya Shabi, Victoria K. Baxter, Nathan P. Crilly, Michael McLane, Diane E. Griffin, Andrew Pekosz, Sabra L. Klein, Irina Burd
NATURE COMMUNICATIONS
(2017)
Article
Infectious Diseases
Paul Jacob Bueno de Mesquita, Jonathan Nguyen-Van-Tam, Ben Killingley, Joanne Enstone, Robert Lambkin-Williams, Anthony S. Gilbert, Alexander Mann, John Forni, Jing Yan, Jovan Pantelic, Michael L. Grantham, Donald K. Milton
Summary: This study compared viral aerosol shedding between volunteers nasally inoculated and naturally infected with influenza A/H3N2. It found that natural cases had significantly higher aerosol shedding than experimental cases. Certain influenza-like illness-related symptoms were associated with viral aerosol shedding.
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2021)
Article
Microbiology
Jonathan S. Nguyen-Van-Tam, Ben Killingley, Joanne Enstone, Michael Hewitt, Jovan Pantelic, Michael L. Grantham, P. Jacob Bueno de Mesquita, Robert Lambkin-Williams, Anthony Gilbert, Alexander Mann, John Forni, Catherine J. Noakes, Min Z. Levine, LaShondra Berman, Stephen Lindstrom, Simon Cauchemez, Werner Bischoff, Raymond Tellier, Donald K. Milton
Article
Microbiology
Jessica D. Resnick, Michael A. Beer, Andrew Pekosz
Summary: This study investigated the effect of temperature on host responses in human nasal epithelial cells during respiratory virus infections. The results showed that temperature affected SARS-CoV-2, but not influenza A virus, replication fitness, and SCV2-infected cultures had a slower infection-induced response. Furthermore, temperature not only changed the basal transcriptomic landscape of the cells but also impacted the response to infection. Overall, these findings provide new insights into the innate immune response to respiratory infections and may aid in the development of novel treatment strategies.
Article
Virology
Jo L. Wilson, Elgin Akin, Ruifeng Zhou, Anne Jedlicka, Amanda Dziedzic, Hsuan Liu, Katherine Z. J. Fenstermacher, Richard E. Rothman, Andrew Pekosz
Summary: Understanding influenza B virus infections is crucial for controlling severe influenza and related diseases. Recent research has found that the two genetic lineages of influenza B virus differ not only in their antigenic structure but also in their ability to induce host immune responses.
Article
Health Care Sciences & Services
Anna Russell, Edbert B. Hsu, Katherine Z. J. Fenstermacher, Erin P. Ricketts, Gabriella Dashler, Allison Chen, Kathryn Shaw-Saliba, Patrizio P. Caturegli, Andrew Pekosz, Richard E. Rothman
Summary: This study investigated the prevalence of SARS-CoV-2 infection among emergency department healthcare workers in Baltimore during the early stage of the COVID-19 pandemic. The results showed that the infection rate was relatively low and most infected individuals did not require hospitalization. The study also found that the absenteeism among healthcare workers was not significant.
Article
Education, Scientific Disciplines
Michael L. Grantham, Matthew F. McCown, Andrew Pekosz
JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION
(2019)