Article
Cell Biology
Sharon Eisenberg, Ehud Haimov, Glenn F. W. Walpole, Jonathan Plumb, Michael M. Kozlov, Sergio Grinstein
Summary: Anionic phospholipids on biological membranes can affect the electric charge distribution and ion concentration, but little is known about the electrostatic properties of different organellar membranes. Research shows that various organelles have different surface charges, with the inner leaflet of cell membranes being the most negative, while the endoplasmic reticulum has the most positive surface potential.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Plant Sciences
Rosa L. Lopez-Marques, James A. Davis, Jeffrey F. Harper, Michael Palmgren
Summary: The lipid bilayer of biological membranes is complex and asymmetric, with protein P4 ATPase playing a role in generating this asymmetry. Arabidopsis contains 12 P4 ATPases, with studies confirming their essential role in basal cellular functions.
Article
Cell Biology
Riddhi Atul Jani, Aurelie Di Cicco, Tal Keren-Kaplan, Silvia Vale-Costa, Daniel Hamaoui, Ilse Hurbain, Feng-Ching Tsai, Mathilde Di Marco, Anne-Sophie Mace, Yueyao Zhu, Maria Joao Amorim, Patricia Bassereau, Juan S. Bonifacino, Agathe Subtil, Michael S. Marks, Daniel Levy, Graca Raposo, Cedric Delevoye
Summary: Intracellular trafficking is mediated by transport carriers that originate by membrane remodeling from donor organelles. Phosphatidylinositol-4-phosphate (PI4P) and biogenesis of lysosome-related organelles complex 1 (BLOC-1) play important roles in the formation, stability, and functions of recycling endosomal tubules.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Qiongjie Zhou, Qiaomei Wang, Haiping Shen, Yiping Zhang, Shikun Zhang, Xiaotian Li, Ganesh Acharya
Summary: A substantial proportion of Chinese women planning pregnancy were susceptible to cytomegalovirus before conception, with low to moderate levels of immunoglobulin G seropositivity and significant geographic variation. Regional differences in seropositivity should be considered in the integration of cytomegalovirus antibody testing in preconception screening programs to develop prevention strategies targeting congenital infections.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Plant Sciences
Femke de Jong, Teun Munnik
Summary: Membranes are crucial for cell and organelle functions, providing electrochemical energy, creating compartmentalized microenvironments, and facilitating molecule transport and cell signaling. Advances in protein crystallography, lipid-binding studies, and subcellular localization analyses have greatly enhanced our understanding of membrane functions and signaling pathways.
Article
Ophthalmology
Romell B. Gletten, Lee S. Cantrell, Sujoy Bhattacharya, Kevin L. Schey
Summary: AQP5 is localized to spheroidal, linear cytoplasmic vesicles in differentiating bovine lens fiber cells. These vesicles incorporate LC3B and presumably fuse with LIMP-2-positive lysosomes during fiber cell differentiation. Our data suggests a novel mechanism of AQP5 trafficking to the plasma membrane through lysosome-associated unconventional protein secretion.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Immunology
Trung H. M. Pham, Denise M. Monack
Summary: Macrophages function as tissue-immune sentinels and mediate key antimicrobial responses. They can act as a cellular niche for intracellular bacteria. Recent studies show that Salmonella and other intracellular bacteria inject virulence effectors into the cellular cytoplasm to reprogram macrophages into a permissive niche. Further research is needed to understand macrophage functional diversity and immunobiology.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alba Ferre, Francois Chauvigne, Anna Vlasova, Birgitta Norberg, Luca Bargelloni, Roderic Guigo, Roderick Nigel Finn, Joan Cerda
Summary: The aquaporin-mediated oocyte hydration plays a crucial role in the evolution of pelagic eggs and the success of marine teleosts. However, the molecular regulatory mechanisms controlling this process have not been fully understood. Through analyzing over 400 piscine genomes, researchers discovered a teleost-specific aquaporin-1 cluster (TSA1C) consisting of aqp1aa-aqp1ab2-aqp1ab1 genes. Evolutionary analysis revealed that marine species with highly hydrated pelagic eggs retained at least one downstream paralog, and a significant portion of modern marine teleosts retained both aqp1ab-type channels and co-evolved regulatory proteins for membrane trafficking. The selective advantages of these mechanisms were demonstrated to accelerate hydration and facilitate geodispersal in oceanic currents.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Review
Plant Sciences
Marijke R. Murray, Steffen P. Graether
Summary: Plants have evolved mechanisms, such as the expression of late embryogenesis abundant (LEA) proteins, to protect against damage caused by cold temperatures. One of the most studied LEA proteins is the type II LEA protein, known as dehydrins. Dehydrins help prevent membrane damage by binding to membranes during abiotic stress and lowering the transition temperature. The structure and interactions of dehydrins with membranes have been investigated using biophysical techniques.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Pediatrics
Irene Arduino, Javier Calvo, Massimo Ritta, Sergio Cabeza, Marta Llobera, David Lembo, Antoni Gaya, Manuela Donalisio
Summary: The study shows that lowering the time and temperature of pasteurization can better preserve the bioactive components of human milk and restore its antiviral activity against cytomegalovirus. This work questions the standard pasteurization method and opens the debate on whether the temperature commonly used in human milk banks should be lowered for better preservation of the biological components of the milk.
PEDIATRIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Shimi Meleppattu, Haixia Zhou, Jin Dai, Miao Gui, Alan Brown
Summary: This study determined the structures of native IFT-A complexes using cryo-EM and found that subcomplex rearrangements enable IFT-A to polymerize on anterograde IFT trains. The study also discovered that binding of IFT-A to IFT-B shields the preferred lipid-binding interface and orients a network of b-propeller domains towards the ciliary membrane, capable of accommodating diverse cargoes.
Article
Obstetrics & Gynecology
Luke A. Gatta, Eric Rochat, Jeremy M. Weber, Sarah Valencia, Alaattin Erkanli, Sarah K. Dotters-Katz, Sallie Permar, Brenna L. Hughes
Summary: The study found that about 75% of pregnant women who were positive for cytomegalovirus IgG shed the virus at some point during gestation. The only factor associated with shedding was nulliparity.
AMERICAN JOURNAL OF OBSTETRICS & GYNECOLOGY MFM
(2022)
Article
Gastroenterology & Hepatology
Chloe De Broucker, Aurelie Plessier, Isabelle Ollivier-Hourmand, Sebastien Dharancy, Christophe Bureau, Jean-Paul Cervoni, Philippe Sogni, Odile Goria, Olivier Corcos, Riccardo Sartoris, Maxime Ronot, Valerie Vilgrain, Emmanuelle de Raucourt, Kamal Zekrini, Hortense Davy, Francois Durand, Audrey Payance, Nadira Fidouh-Houhou, Yazdan Yazdanpanah, Dominique Valla, Pierre-Emmanuel Rautou
Summary: Recent PVT patients with features of mononucleosis syndrome may have CMV disease. CMV disease does not affect the extension or recanalization of thrombosis, but CMV positive patients are more likely to carry the prothrombin G20210A gene variant.
JOURNAL OF HEPATOLOGY
(2022)
Article
Multidisciplinary Sciences
Regina B. Troyanovsky, Alina P. Sergeeva, Indrajyoti Indra, Chi-Shuo Chen, Rei Kato, Lawrence Shapiro, Barry Honig, Sergey M. Troyanovsky
Summary: The study explores the structure and function of the multiprotein complex formed by the cytoplasmic tails of classical cadherins in adherens junctions. It reveals that cytoplasmic proteins interact with this complex and organize into clusters, potentially synchronizing signaling networks among neighboring cells within tissues.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Gastroenterology & Hepatology
Sarah Kemme, Jennifer D. Canniff, Amy G. Feldman, Krystle M. Garth, Shaobing Li, Zhaoxing Pan, Ronald J. Sokol, Adriana Weinberg, Cara L. Mack
Summary: This study aimed to investigate the relationship between cytomegalovirus (CMV) infection and the severity of disease and pretransplant outcomes in infants with biliary atresia (BA). The results showed that CMV infection was associated with worse liver injury, delayed surgery, and increased risk of pretransplant death, but had no effect on native liver survival.
HEPATOLOGY COMMUNICATIONS
(2023)