Article
Multidisciplinary Sciences
Kai Januschowski, Sven Schnichels, Jose Hurst, Christine Hohenadl, Charlotte Reither, Annekatrin Rickmann, Lisa Pohl, Karl-Ulrich Bartz-Schmidt, Martin S. Spitzer
Article
Parasitology
Ahmed Thabet, Ronald Schmaeschke, Jasmin Fertey, Berit Bangoura, Jessy Schoenfelder, Matthias Lendner, Sebastian Ulbert, Arwid Daugschies
VETERINARY PARASITOLOGY
(2019)
Article
Ophthalmology
Patricia Klemm, Jose Hurst, Matthias Dias Blak, Thoralf Herrmann, Marion Melchinger, Karl U. Bartz-Schmidt, Guenther Zeck, Maximilian Schultheiss, Martin S. Spitzer, Sven Schnichels
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2019)
Article
Oncology
Jasmin Fertey, Joerg Hagmann, Hans-Joachim Ruscheweyh, Christian Munk, Susanne Kjaer, Daniel Huson, Juliane Haedicke-Jarboui, Frank Stubenrauch, Thomas Iftner
Article
Multidisciplinary Sciences
Jasmin Fertey, Martin Thoma, Jana Beckmann, Lea Bayer, Julia Finkensieper, Susann Reisshauer, Beatrice Sarah Berneck, Leila Issmail, Jessy Schoenfelder, Javier Portillo Casado, Andre Poremba, Frank-Holm Roegner, Bastian Standfest, Gustavo R. Makert, Lia Walcher, Ann-Kathrin Kistenmacher, Stephan Fricke, Thomas Grunwald, Sebastian Ulbert
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Beatrice Sarah Berneck, Alexandra Rockstroh, Jasmin Fertey, Thomas Grunwald, Sebastian Ulbert
Article
Biochemistry & Molecular Biology
Sven Schnichels, Maximilian Schultheiss, Patricia Klemm, Matthias Blak, Thoralf Herrmann, Marion Melchinger, Karl-Ulrich Bartz-Schmidt, Marina Loescher, Guenther Zeck, Martin Stehphan Spitzer, Jose Hurst
Summary: This study investigated the neuroprotective effects of Cyclosporine A (CsA) on rat retinas, showing that a concentration of 9 μg/mL CsA had significant neuroprotective effects on retinal ganglion cells under hypoxic conditions without negative effects. Further in vivo studies are warranted to explore the potential of local CsA treatment for hypoxic retinal diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Marina Loescher, Chiara Seiz, Jose Hurst, Sven Schnichels
Summary: This review discusses the challenges of topical drug delivery to the posterior segment of the eye. It focuses on drug characteristics, ocular barriers, animal models, and delivery systems to improve ocular drug delivery. Promising results suggest the possibility of topical treatment for retinal diseases, calling for further research.
Article
Biochemistry & Molecular Biology
Alice Rocha Teixeira Netto, Jose Hurst, Karl-Ulrich Bartz-Schmidt, Sven Schnichels
Summary: A porcine ex vivo dry eye model was established in this study by incubating porcine corneas in low humidity. The model exhibited increased expression of dry eye markers and higher cell death rate compared to the controls. Treatment with dexamethasone and hyaluronic acid was able to reverse some of these effects. The model provides a valuable tool for animal-free testing of novel dry eye treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Natalie Wagner, Armin Safaei, Jose Hurst, Pia A. Vogt, H. Burkhard Dick, Stephanie C. Joachim, Sven Schnichels
Summary: This study established a porcine organotypic co-cultivation model to investigate the interactions between photoreceptor and RPE cells. The results showed that co-cultivation had beneficial effects on the neuroretina, preserving photoreceptors and maintaining synaptic vesicles in vitro.
Article
Biochemistry & Molecular Biology
Agnes Fietz, Jose Hurst, Sven Schnichels
Summary: Awareness of the risks of blue light exposure is increasing due to the use of blue light-enriched LEDs in displays. Blue light has been linked to eye diseases such as dry eye, cataracts, and age-related macular degeneration. While the effects of blue light on retinal pigment epithelium cells and photoreceptors have been extensively studied, less is known about its effects on Muller cells. Muller cells play crucial roles in supporting the retina, but their reaction to stress has been assumed to be secondary. However, this study found that increased oxidative stress caused by blue light exposure can significantly harm Muller cell function. Red light was found to have a protective effect, partially preventing cell damage caused by blue light exposure. This research suggests that preventing cell death of Muller cells could be a promising therapy to enhance retinal survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Sven Schnichels, David Simmang, Marina Loescher, Andreas Herrmann, Jan Willem de Vries, Martin S. Spitzer, Jose Hurst
Summary: Retinal eye diseases are a major cause of blindness in the Western world, and current treatments involving intravitreal injections of concentrated drugs have numerous drawbacks. In this study, lipid-DNA nanoparticles were investigated as a potential carrier system for delivering drugs to the posterior part of the eye. The nanoparticles exhibited excellent biodistribution and retention, reaching the retina within minutes and offering a potentially safe and long-term solution for small molecule or nucleotide-based therapies.
Article
Biochemical Research Methods
Alice Rocha Teixeira Netto, Marc Dieter Hrusa, Karl-Ulrich Bartz-Schmidt, Sven Schnichels, Jose Hurst
Summary: In ophthalmic research, there is a need for in vitro corneal cell models. Two different methods, the outgrowth and collagenase method, were described for cultivating primary corneal cells from porcine eyes. The outgrowth method requires fewer eyes and less time compared to the collagenase method, while the collagenase method yields mature cells earlier, within 2-3 weeks.
METHODS AND PROTOCOLS
(2023)
Article
Multidisciplinary Sciences
Ana M. Maliha, Sandra Kuehn, Jose Hurst, Fenja Herms, Michael Fehr, Karl U. Bartz-Schmidt, H. Burkhard Dick, Stephanie C. Joachim, Sven Schnichels
SCIENTIFIC REPORTS
(2019)
Article
Immunology
Alexandra Rockstroh, Johannes Wolf, Jasmin Fertey, Sven Kalbitz, Stefanie Schroth, Christoph Luebbert, Sebastian Ulbert, Stephan Borte
Summary: Studying the humoral protective immune response post SARS-CoV-2 infections is crucial for assessing reinfection risk, improving diagnostic methods, and evaluating vaccine trials. This research analyzed the dynamics of neutralizing antibodies and IgG responses in COVID-19 patients, identifying potential diagnostic markers for estimating neutralizing protection.
EMERGING MICROBES & INFECTIONS
(2021)