Article
Immunology
Han Cao, Yunfei Wang, Ning Luan, Kangyang Lin, Cunbao Liu
Summary: Comparing the humoral and cellular immunity induced by different gE mRNA vaccines, the study found that the C-terminal double mutant of gE showed stable advantages in all indicators tested, making it a candidate for both safer varicella vaccines and more effective zoster vaccines.
Article
Biology
Tingting Chen, Jie Sun, Sibo Zhang, Tingting Li, Liqin Liu, Wenhui Xue, Lizhi Zhou, Siting Liang, Zhili Yu, Qingbing Zheng, Hai Yu, Tong Cheng, Jun Zhang, Ying Gu, Shaowei Li, Ningshao Xia
Summary: We successfully produced a VZV gE immunogen using an E. coli expression system. By truncating the C-terminal of the protein, we improved its solubility and yield, making it easier to purify for vaccine manufacturing. The lead truncated gE (aa 31-358), referred to as tgE, showed excellent antigenicity as a monomer in solution.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Immunology
Junli Li, Lili Fu, Xiaonan Guo, Yang Yang, Jiaxin Dong, Guozhi Wang, Aihua Zhao
Summary: BC02 is a novel compound adjuvant that enhances the immune response to varicella-zoster virus, and it also activates innate immune response.
Article
Microbiology
Yan Niu, Aiping Wang, Jingming Zhou, Hongliang Liu, Yumei Chen, Peiyang Ding, Yanhua Qi, Chao Liang, Xifang Zhu, Gaiping Zhang
Summary: An indirect ELISA kit was developed for detecting anti-VZV antibodies in clinical samples, with the VZV membrane glycoprotein E as the target antigen. The kit showed high coincidence rate and stability in comparison tests.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Wenhui Xue, Tingting Li, Sibo Zhang, Yingbin Wang, Minqing Hong, Lingyan Cui, Hong Wang, Yuyun Zhang, Tingting Chen, Rui Zhu, Zhenqin Chen, Lizhi Zhou, Rongwei Zhang, Tong Cheng, Qingbing Zheng, Jun Zhang, Ying Gu, Ningshao Xia, Shaowei Li
Summary: In this study, a new immunogen for vaccine design was created by displaying VZV-gE on the baculovirus surface. The engineered baculovirus demonstrated correct localization and display of gE. The resulting vaccine showed excellent antigenicity against VZV and induced stronger immune responses in mice, making it a promising candidate for HZ vaccine development.
Article
Virology
Jie Liu, Junyang Lin, Linjun Cai, Jie Sun, Xue Ding, Cenrong Wang, Yanchun Wu, Xiaoling Gao, Weiheng Su, Chunlai Jiang
Summary: This study developed HZ DNA vaccines and found that each vaccine induced specific antibody production. The IE63-2A-gE vaccine elicited immune responses comparable to the current HZ vaccine, making it a candidate for HZ vaccine development.
Article
Immunology
Junli Li, Lili Fu, Yang Yang, Guozhi Wang, Aihua Zhao
Summary: Herpes zoster is a disease caused by Varicella-zoster virus. There are currently two vaccines available for prevention, but one loses effectiveness with age. A new adjuvant has been developed to enhance vaccine efficacy and provide long-term protection.
Article
Microbiology
Tomohiko Sadaoka, Daniel P. Depledge, Labchan Rajbhandari, Judith Breuer, Arun Venkatesan, Jeffrey Cohen
Summary: The variant allele in the glycoprotein B (gB) gene plays a crucial role in the attenuation of the varicella vaccine. This variant impairs fusion, virus entry into neurons, and replication in human skin cells. Understanding the molecular basis for vaccine attenuation could aid in the development of other herpesvirus vaccines.
Article
Virology
Aiping Wang, Yan Niu, Jianguo Zhao, Hongliang Liu, Peiyang Ding, Yumei Chen, Jingming Zhou, Xifang Zhu, Ying Zhang, Chao Liang, Gaiping Zhang
Summary: In this study, monoclonal antibodies against VZV gE protein were generated by immunizing mice. A colloidal gold-based immunochromatographic strip was developed for rapid detection of VZV, with high sensitivity and specificity.
Article
Immunology
Han Cao, Yunfei Wang, Ning Luan, Cunbao Liu
Summary: The study demonstrates the potential of vaccines combining LNPs and immunostimulatory nucleic acids as safe and economical candidates for varicella and zoster vaccines, showing efficient induction of cell-mediated immunity and antibody production.
Article
Immunology
Yunhwa Kim, Ji-Young Hwang, Kyung-Min Lee, Eunsil Lee, Hosun Park
Summary: The prevalence of varicella is high among 4-6 year old children in South Korea, with some vaccinated children experiencing breakthrough varicella. Vaccinated 1-year-olds showed an increase in antibody titers post-vaccination, while older children had higher titers with age.
Article
Microbiology
Arnaud John Kombe Kombe, Jiajia Xie, Ayesha Zahid, Huan Ma, Guangtao Xu, Yiyu Deng, Fleury Augustin Nsole Biteghe, Ahmed Mohammed, Zhao Dan, Yunru Yang, Chen Feng, Weihong Zeng, Ruixue Chang, Keyuan Zhu, Siping Zhang, Tengchuan Jin
Summary: A highly sensitive diagnostic kit for varicella-zoster virus (VZV) based on the chemiluminescent immunoassay (CLIA) approach was developed and validated. The kit showed high sensitivity and specificity in detecting VZV-specific immunoglobulins, and the CLIA approach demonstrated overall higher sensitivity compared to ELISA. The kit is simple and suitable for high-throughput routine analysis.
Article
Microbiology
Garima Shahi, Mohit Kumar, Nitesh Kumar Khandelwal, Atanu Banerjee, Parijat Sarkar, Sonam Kumari, Brooke D. Esquivel, Neeraj Chauhan, Amitabha Chattopadhyay, Theodore C. White, Naseem A. Gaur, Ashutosh Singh, Rajendra Prasad
Summary: By disrupting the orthologs of ScIpt1 and ScSkn1 in Candida glabrata, we have specifically blocked a key step of sphingolipid biosynthesis and found that it significantly affects the azole susceptibility of the organism.
Article
Pharmacology & Pharmacy
Ning Luan, Han Cao, Yunfei Wang, Kangyang Lin, Cunbao Liu
Summary: It has been found that using LNPs as vaccine carriers can enhance the synergistic adjuvant effect of CpG ODNs and QS21 on VZV-gE, potentially leading to the development of a novel varicella vaccine.
Article
Biotechnology & Applied Microbiology
Kwang Sung Kim, Shin Ae Park, Seo Ri Wui, Ara Ko, Na Gyong Lee
Summary: This study optimized the culture media conditions for CHO cell growth and gE protein production, leading to a significant increase in gE protein yield compared to original culture conditions. The selected media combination was used for scaled-up production of VZV gE antigen for an HZ subunit vaccine currently in phase I clinical trial, providing valuable insights for future culture media optimization for CHO cells expressing recombinant vaccine antigens.