Article
Virology
Mayra Colunga-Saucedo, Edson I. Rubio-Hernandez, Miguel A. Coronado-Ipina, Sergio Rosales-Mendoza, Claudia G. Castillo, Mauricio Comas-Garcia
Summary: In this study, three plasmids were generated for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), a helper plasmid coding for the structural proteins, and a replicon plasmid coding for nonstructural proteins. All plasmids contain a reporter gene (mKate2). Co-transfection of the helper and replicon plasmids has biotechnological/biomedical applications as it enables the delivery of self-replicating RNA for transient gene expression in target cells.
Article
Medicine, Research & Experimental
Patrick C. Freitag, Meike Kaulfuss, Lea Fluhler, Juliane Mietz, Fabian Weiss, Dominik Brucher, Jonas Kolibius, Sheena N. Smith, Christian Munz, Obinna Chijioke, Andreas Pluckthun
Summary: Clinical success in T cell therapy has prompted efforts to improve safety and efficacy, and expand its use in solid tumors. However, limitations in viral vectors, such as limited payload capacity and specificity of transduction, hinder progress in cell therapy. This study developed a combination of adapter constructs for targeted transduction of human T cells by adenoviral vectors, overcoming these limitations and increasing payload capacity. These findings provide a potential avenue to overcome current limitations of T cell therapy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Biotechnology & Applied Microbiology
Wenbo Zhu, Jiankai Liang, Jingyi Tan, Li Guo, Jing Cai, Jun Hu, Guangmei Yan, Yang Liu, Jiayu Zhang, Deli Song, Jia Dan, Chun-Wa Wong, Xingwen Su, Pengxin Qiu, Yuan Lin
Summary: Researchers successfully constructed a fluorescent reporter virus for real-time visualization and quantification of Alphavirus M1 virus both in vitro and in vivo. The reporter-encoding virus maintained the characteristics of parental virus and the fluorescence was positively correlated with virus replication.
HUMAN GENE THERAPY
(2021)
Review
Agriculture, Dairy & Animal Science
Jia Chen, Shuhua Yang, Peng Li, Aibo Wu, Eugenie Nepovimova, Miao Long, Wenda Wu, Kamil Kuca
Summary: This review focuses on the toxicological mechanisms of four major mycotoxins and the role of microRNAs in these mechanisms. By studying the regulation of miRNAs, harmful effects of mycotoxins can be prevented and controlled, providing a theoretical basis for the prevention and treatment of related diseases.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Sadek Ahmed, Maha M. Amin, Sarah Mohamed El-Korany, Sinar Sayed
Summary: The purpose of this study was to develop and optimize Fenticonazole Nitrate-loaded novasomes for enhanced corneal penetration and antifungal activity. The optimized formula showed high entrapment efficiency, improved corneal permeation, and increased antifungal activity. The results confirmed the validity of novasomes in improving the corneal penetration and antifungal activity of Fenticonazole Nitrate.
Article
Virology
Marco Brustolin, Sujit Pujhari, Gerard Terradas, Kristine Werling, Sultan Asad, Hillery C. C. Metz, Cory A. A. Henderson, Donghun Kim, Jason L. L. Rasgon
Summary: Epidemiological and clinical studies show that multiple arboviruses are circulating simultaneously in human populations, leading to coinfection or superinfection in both humans and mosquitoes. This study investigates the interaction dynamics between Mayaro virus and Zika virus in vertebrate and mosquito cells, as well as Aedes aegypti mosquitoes. The results demonstrate that Aedes aegypti mosquitoes can be simultaneously infected with and transmit both viruses, with Mayaro virus having a negative impact on Zika virus infection and dissemination. These findings highlight the importance of studying coinfection and superinfection dynamics in epidemic areas.
JOURNAL OF VIROLOGY
(2023)
Article
Medicine, Research & Experimental
Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang
Summary: This study developed CB-activatable cASOs and successfully inhibited disease-related genes in tumor cells with limited effects on normal cells. Compared to linear cASOs, CB-activatable cASOs also reduced nonspecific immunostimulation.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biotechnology & Applied Microbiology
Jae Geun Song, Sang Hoon Lee, Hyo-Kyung Han
Summary: The study aimed at developing an M cell targeted-nanocomposite delivery system for protein drugs to overcome absorption barriers in the GI tract, and showed promising results in improving the bioavailability of orally administered protein drugs in mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Virology
Na Li, Zhen Wang, Rui Wang, Zhe-Rui Zhang, Ya-Nan Zhang, Cheng-Lin Deng, Bo Zhang, Lu-Qing Shang, Han-Qing Ye
Summary: Lycorine can inhibit CHIKV in cell culture without cytotoxicity and exhibits broad-spectrum anti-alphavirus activity at an early post-entry stage of CHIKV life cycle.
Article
Biochemistry & Molecular Biology
Sweta Khanal, Matthew de Cruz, Britton Strickland, Kody Mansfield, Eric C. Lai, Alex Flynt
Summary: This study reveals that miR-1017, a highly expressed mirtron in Drosophila, can extend the lifespan of neurons through targeting its host transcript and affecting splicing.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Eman M. Abd-Elmonem, Amna M. A. Makky, Assem Antar, Wessam H. Abd-Elsalam, Islam A. Khalil
Summary: This study developed a new ocular drug formulation by incorporating AMO into mixed micelles, aiming to enhance the delivery of AMO to the corneal tissue. The optimal formulation F5 showed a particle size of 26.55 +/- 1.68 nm and a drug loading percentage of 7.49 +/- 0.01%. The ex vivo permeation study confirmed its corneal penetrability, and the MIC of the optimal formulation was 3 folds lower than the standard market product. In vivo study on rabbits demonstrated the biosafety and effectiveness of the optimal formulation. Overall, AMO-MMs hold promise as a corneal delivery system for the management of fungal keratitis.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Anna Costanzini, Chiara Ruzza, Joaquim Azevedo Neto, Chiara Sturaro, Davide Malfacini, Catia Sternini, Roberto De Giorgio, Girolamo Calo
Summary: Opioid-induced constipation is a common adverse effect of opioid drugs. Naloxegol, a peripherally acting mu opioid receptor antagonist, has been shown to be effective in counteracting the constipating effects of fentanyl in both in vitro and in vivo experiments. Through in vitro and in vivo studies, naloxegol demonstrated competitive mu receptor antagonist properties and useful PAMORA action for treating opioid-induced constipation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Keyan Sun, Lei Zhang, Peng Chen, Debin Qi, Hao Liu, Haili Bao, Xiongwei Wang, Tao Li
Summary: In this study, abnormal expression of circular RNA circSETD2 in liver cancer tissues was found, and its knockdown was shown to promote invasion and proliferation of liver cancer cells. Additionally, circSETD2 knockdown was also found to inhibit the expression of specific genes. These findings contribute to our understanding of the mechanisms underlying liver cancer progression.
Article
Chemistry, Applied
Chen Jiang, Yingxue Li, Lingjuan Yan, Aiying Ye, Qiong He, Cheng Yao
Summary: The C3H probe can quickly respond to HOCl, with high sensitivity and low detection limit, as well as high selectivity and anti-interference properties. It can also monitor both exogenous and endogenous HOCl in mitochondria effectively. Additionally, in vivo imaging experiments on nude mice demonstrate the probe's ability to detect exogenous ClO-.
Review
Biochemistry & Molecular Biology
Mohammad Aslam, Beenish Fakher, Yuan Qin
Summary: In living organisms, sexual reproduction relies on the successful development of gametes, with flowering plants producing gametes in specialized organs of the flower, the gametophytes. Recent evidence suggests that small RNA-mediated pathways play important roles in the development and specification of the female gametophyte. Numerous protein complexes, molecular, and genetic mechanisms are involved in tightly regulating the development of the female gametophyte, known as the embryo sac, in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Elizabeth K. Wansley, Mala Chakraborty, Kenneth W. Hance, Michael B. Bernstein, Amanda L. Boehm, Zhimin Guo, Deborah Quick, Alex Franzusoff, John W. Greiner, Jeffrey Schlom, James W. Hodge
CLINICAL CANCER RESEARCH
(2008)
Article
Oncology
Mala Chakraborty, Elizabeth K. Wansley, Jorge A. Carrasquillo, Sarah Yu, Chang H. Paik, Kevin Camphausen, Michael D. Becker, William F. Goeckeler, Jeffrey Schlom, James W. Hodge
CLINICAL CANCER RESEARCH
(2008)
Article
Immunology
Michael B. Bernstein, Mala Chakraborty, Elizabeth K. Wansley, Zhimin Guo, Alex Franzusoff, Sven Mostboeck, Helen Sabzevari, Jeffrey Schlom, James W. Flodge
Article
Oncology
Chie Kudo-Saito, Charlie T. Garnett, Elizabeth K. Wansley, JeVrey Schlom, James W. Hodge
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2007)
Article
Biochemistry & Molecular Biology
Hadley J. Sharp, Elizabeth K. Wansley, Charlie T. Garnett, Mala Chakraborty, Kevin Camphausen, Jeffrey Schlom, James W. Hodge
FRONTIERS IN BIOSCIENCE-LANDMARK
(2007)
Article
Oncology
Chie Kudo-Saito, Elizabeth K. Wansley, M. Eilene Gruys, Robert Wiltrout, Jeffrey Schlom, James W. Hodge
CLINICAL CANCER RESEARCH
(2007)
Article
Biotechnology & Applied Microbiology
Patrick J. Dillon, Elizabeth K. Wansley, Virginia A. Young, Martha A. Alexander-Miller, Griffith D. Parks
JOURNAL OF GENERAL VIROLOGY
(2006)
Article
Immunology
Eric A. Reits, James W. Hodge, Carla A. Herberts, Tom A. Groothuis, Mala Chakraborty, Elizabeth K. Wansley, Kevin Camphausen, Rosalie M. Luiten, Arnold H. de Ru, Joost Neijssen, Alexander Griekspoor, Elly Mesman, Frank A. Verreck, Hergen Spits, Jeffrey Schlom, Peter van Veelen, Jacques J. Neefjes
JOURNAL OF EXPERIMENTAL MEDICINE
(2006)
Article
Virology
EK Wansley, PJ Dillon, MD Gainey, J Tam, SD Cramer, GD Parks
Article
Virology
EK Wansley, JM Grayson, GD Parks
Article
Virology
EK Wansley, GD Parks
JOURNAL OF VIROLOGY
(2002)
Article
Virology
GD Parks, VA Young, C Koumenis, EK Wansley, JL Layer, KM Cooke