期刊
JOURNAL OF VIROLOGY
卷 83, 期 17, 页码 8849-8858出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00339-09
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资金
- Comunidad de Madrid
- Fundacion Botin and Juan de la Cierva Program
- IFARHU-SENACYT Program from Panama
- JAE CSIC Program
- Ministerio de Educacion y Ciencia [BFU 2005-04091]
- Fondo de Investigaciones Sanitarias, CIBERhed
- Spanish Ministry of Education and Science [BIO2005-00599, BFU2008-03784]
- Fundacion de Investigacion Medica Mutua Madrilena
Infection by herpesviruses causes a dramatic disturbance of PML oncogenic domains (PODs) that has been suggested to be essential for viral lytic replication. Several proteins from Kaposi's sarcoma-associated herpesvirus ( KSHV) have been tested as putative POD-disrupting factors with negative results. Here, we show that LANA2, a viral protein that is absolutely required for the viability and proliferation of KSHV-infected primary effusion lymphoma (PEL) cells, increases the levels of SUMO2-ubiquitin-modified PML and induces the disruption of PODs by a proteasome-mediated mechanism. In addition, we demonstrate that this disruption is largely dependent on both the integrity of a SUMO interaction motif in LANA2 and the lysine 160 from PML. Moreover, silencing of LANA2 expression in PEL cells by RNA interference led to an increase in the PML levels. Finally, we demonstrate that LANA2 relieves PML-mediated transcriptional repression of survivin, a protein that directly contributes to malignant progression of PEL. This represents the first example of inactivation of these important antiviral structures by KSHV.
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