Article
Microbiology
Ki-Jong Kang, Dong-Hwan Kim, Eui-Ju Hong, Hyun-Jin Shin
Summary: This study found a correlation between the serum neutralizing test (SNT) and ELISA in PED-vaccinated sow sera, with higher neutralizing activity sera having higher IgG titers, especially in the S3 region antibody levels. The carboxy-terminal region of the S protein induced stronger neutralizing activity and could be useful for evaluating PED vaccine efficacy.
Article
Pharmacology & Pharmacy
Jiaxuan Chen, Jia Hou, Rong Na, Bin Zhou, Jinlin Hou, De-Ke Jiang
Summary: This study found that overexpression of BST2 can improve the anti-HBV effect of IFN-α treatment, and BST2_rs9576 is an effective indicator for predicting the therapy response of PegIFN alpha-treated patients with CHB. The PGS model has the potential to enhance the ability of PegIFN alpha therapy response.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Chemistry, Analytical
Yuwei Wang, Linbin Li, Zihe Dong, Yan Yu, Aie Zhou, Xiaofen Zhao, Juan Zhang
Summary: A novel electrochemical biosensor was developed for ultrasensitive detection of HCV core antigen (HCVcAg) using terminal deoxynucleotidyl transferase (TdT) amplification and DNA nanowires (DNW). The biosensor achieved a wide linear range for HCVcAg detection from 0.1 to 312.5 pg/mL with a low limit of detection of 32 fg/mL, demonstrating good practicality for use in clinics.
Article
Biochemistry & Molecular Biology
Aikaterini I. Argyriou, Georgios A. Machaliotis, Garyfallia I. Makrynitsa, Eleni G. Kaliatsi, Constantinos Stathopoulos, Georgios A. Spyroulias
Summary: The La protein is an RNA-binding protein found in all human cells, mainly localized in the nucleus. It interacts with RNA polymerase III transcripts and modulates subsequent processing events. Export of La to the cytoplasm stimulates the decoding of specific cellular and viral mRNAs through IRES-dependent binding and translation. Atomic-level-resolution structural insights on human La protein in solution and evidence about the role of the C-terminal domain in recognizing HCV-IRES are provided using NMR spectroscopy and ITC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Caroline Lefeuvre, Helene Le Guillou-Guillemette, Alexandra Ducancelle
Summary: Chronic HBV infection is a common factor associated with HCC, with studies showing that the HBc protein plays a significant role in affecting pathways involved in HCC development, such as migration, proliferation, apoptosis, metabolism, and immune responses. HBc can also promote HCC metastasis by modulating host gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Suchanat Boonkaew, Abdulhadee Yakoh, Nattaya Chuaypen, Pisit Tangkijvanich, Sirirat Rengpipat, Weena Siangproh, Orawon Chailapakul
Summary: Electrochemical paper-based analytical devices (ePADs) are simple, portable, and low-cost analytical devices for point-of-care testing of clinical biomarkers. A sequential ePAD has been developed for simultaneous detection of hepatitis B surface antigen and hepatitis C core antigen by integrating dual flow behaviors, avoiding the need for complex multi-step reagent manipulation by the user. The proposed ePAD shows high promise as an easy-to-use, portable, and extendable sensor for other multiplex biological assays.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Virology
Savvina Moustafa, Katerina Kassela, Maria Bampali, Nikolas Dovrolis, Athanassios Kakkanas, Apostolos Beloukas, Penelope Mavromara, Ioannis Karakasiliotis
Summary: This study analyzed the role of HCV core protein in TNF alpha-induced death, considering the genetic variability of HCV. The results showed that only HCV 4a core protein increased sensitivity to TNF alpha-induced death. Transgenic mice expressing HCV 4a core protein were more susceptible to the LPS/Dgal model, while mice expressing HCV 4f core protein had the same susceptibility as controls.
Article
Biochemistry & Molecular Biology
Ji-Min Park, Hyunyoung Yoon, Yuna Jeong, Kyung Lib Jang
Summary: P53 inhibits HCV replication by downregulating HCV Core protein levels through enhancing E6AP-mediated ubiquitination and degradation of HCV Core.
Article
Multidisciplinary Sciences
Alexander I. Mosa, David S. Campo, Yury Khudyakov, Mounir G. AbouHaidar, Adam J. Gehring, Atif Zahoor, Jonathan K. Ball, Richard A. Urbanowicz, Jordan J. Feld
Summary: A new strategy has been developed to overcome the genetic diversity of the hepatitis C virus by eliciting broadly neutralizing antibodies. The strategy involves immunizing mice with variants from different clusters of the immunodominant hyper-variable region 1, resulting in sera that can neutralize diverse HCV variants. This approach offers a potential solution for antigenic variability in other highly mutable viruses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Hyunyoung Yoon, Kyung Lib Jang
Summary: This study found that HBV X protein and HCV core protein work together to inhibit the expression of E-cadherin in cells, leading to an increased risk of hepatocellular carcinoma. HBV and HCV increase the level and activity of DNA methyl-transferases, resulting in hypermethylation of the E-cadherin promoter. This leads to epithelial-mesenchymal transition and tumor metastasis.
Article
Virology
Joey McGregor, Joshua M. Hardy, Chan-Sien Lay, Irene Boo, Michael Piontek, Manfred Suckow, Fasseli Coulibaly, Pantelis Poumbourios, Rob J. Center, Heidi E. Drummer
Summary: There is currently no vaccine for hepatitis C virus, but researchers have developed a virus-like particle vaccine that can induce the production of neutralizing antibodies and has shown immunogenicity in guinea pigs. This vaccine platform holds promise as a low-cost candidate for large-scale production of an HCV vaccine.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qing Zhou, Yuhao Yang, Zhanxue Xu, Kai Deng, Zhenzhen Zhang, Jiawei Hao, Ni Li, Yanling Wang, Ziwen Wang, Haihang Chen, Yang Yang, Fei Xiao, Xiaohong Zhang, Song Gao, Yi-Ping Li
Summary: ATAD1 plays a crucial role in maintaining mitochondrial homeostasis and combating viral infection. HCV infection induces mitochondrial fragmentation and enhances the pro-infection effect of ATAD1 knockout. ATAD1 interacts with HCV NS5B protein and promotes its degradation, thereby exerting its antiviral function.
Article
Multidisciplinary Sciences
David Flores-Solis, Irina P. Lushpinskaia, Anton A. Polyansky, Arya Changiarath, Marc Boehning, Milana Mirkovic, James Walshe, Lisa M. Pietrek, Patrick Cramer, Lukas S. Stelzl, Bojan Zagrovic, Markus Zweckstetter
Summary: This study reveals that tyrosine-proline interactions play a crucial role in the temperature and concentration-dependent self-coacervation of the C-terminal domain (CTD) of RNA polymerase II (Pol II). These interactions are responsible for the co-recruitment of the human Mediator complex and CTD during phase separation.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Lenka Stejskal, Mphatso D. Kalemera, Charlotte B. Lewis, Machaela Palor, Lucas Walker, Tina Daviter, William D. Lees, David S. Moss, Myrto Kremyda-Vlachou, Zisis Kozlakidis, Giulia Gallo, Dalan Bailey, William Rosenberg, Christopher J. R. Illingworth, Adrian J. Shepherd, Joe Grove
Summary: This study reveals that the hypervariable region-1 (HVR-1) of the fusion proteins E1E2 in Hepatitis C Virus (HCV) has an autoinhibitory function that controls the activity of the proteins. The mechanism is turned off by host receptor interactions, allowing virus entry. Mutations or deletion of HVR-1 results in enhanced virus entry but increased sensitivity to neutralising antibodies.
Article
Biophysics
Ru Cheng, Fu Zhu, Min Huang, Qiang Zhang, Hui Hong Yan, Xiao Hui Zhao, Fu Kang Luo, Chun Mei Li, Hui Liu, Gao Lin Liang, Cheng Zhi Huang, Jian Wang
Summary: In this study, a new biosensor named hepatitis virus indicator was developed for the simultaneous detection of HBV and HCV. The biosensor utilized Exo III assisted signal amplification strategy and particle counting technology with a dark-field microscopy, enabling sensitive and selective detection.
BIOSENSORS & BIOELECTRONICS
(2022)
