Article
Immunology
Filipe Colaco Mariz, Kerstin Putzker, Peter Sehr, Martin Mueller
Summary: The study presents an L2-oriented approach for measuring antibody-mediated neutralization of multiple HPV types using an optimized high-throughput pseudovirion-based neutralization assay (HT-PBNA) and a highly sensitive L2-peptide capture ELISA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Yury A. Bochkov, Mark Devries, Kaitlin Tetreault, Ronald Gangnon, Sujin Lee, Leonard B. Bacharier, William W. Busse, Carlos A. Camargo, Timothy Choi, Robyn Cohen, Ramyani De, Gregory P. DeMuri, Anne M. Fitzpatrick, Peter J. Gergen, Kristine Grindle, Rebecca Gruchalla, Tina Hartert, Kohei Hasegawa, Gurjit K. Khurana Hershey, Patrick Holt, Kiara Homil, Tuomas Jartti, Meyer Kattan, Carolyn Kercsmar, Haejin Kim, Ingrid A. Laing, Peter N. Le Souef, Andrew H. Liu, David T. Mauger, Tressa Pappas, Shilpa J. Patel, Wanda Phipatanakul, Jacqueline Pongracic, Christine Seroogy, Peter D. Sly, Christopher Tisler, Ellen R. Wald, Robert Wood, Robert F. Lemanske, Daniel J. Jackson, James E. Gern
Summary: This study examined cross-neutralization reactions and antibody duration of rhinovirus type A (RV-A) and type C (RV-C). The results showed limited cross-neutralization reactions between the two types, which are limited to genetically similar strains. However, the duration of antibody response during childhood is similar for both types.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Jie Chen, Daning Wang, Zhiping Wang, Kunbao Wu, Shuangping Wei, Xin Chi, Ciying Qian, Yujie Xu, Lizhi Zhou, Yuqian Li, Sibo Zhang, Tingting Li, Zhibo Kong, Yingbin Wang, Qingbing Zheng, Hai Yu, Qinjian Zhao, Jun Zhang, Ningshao Xia, Shaowei Li, Ying Gu
Summary: Human papillomaviruses (HPV) are associated with cervical cancer and other epithelial tumors. The structure of HPV capsid, composed of major capsid protein L1 and minor capsid protein L2, remains unclear. This study analyzed the residues involved in the coassembly of L1 and L2 and found that certain mutations reduced the amount of L2 protein assembled into the capsid and decreased viral infectivity. Understanding the coassembly between L1 and L2 could contribute to our knowledge of HPV biology and the development of countermeasures against various HPV types by targeting L2 protein.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
Geromine Grange, Marie Mindeguia, Philippe Gisbert, Gilles Meyer
Summary: Control of Bovine Viral Diarrhea Virus types 1 and 2 involves vaccination and removal of infected animals from the herd. A study tested the cross-neutralizing antibody response of heifers vaccinated with Mucosiffa(®) against different strains of BVDV-1 and BVDV-2. The results showed that Mucosiffa(®) vaccination provides stable humoral cross-immunity against all tested BVDV strains.
Article
Virology
Maozhou He, Xin Chi, Zhenghui Zha, Yunbing Li, Yang Huang, Shiwen Huang, Miao Yu, Zhiping Wang, Xinlin Liu, Shuangping Wei, Zekai Li, Tingting Li, Yingbin Wang, Hai Yu, Qinjian Zhao, Jun Zhang, Qingbing Zheng, Ying Gu, Shaowei Li, Ningsha Xia
Summary: This study presents the high-resolution structure of the complete HPV58 pseudovirus (PsV58) determined by cryo-electron microscopy (cryo-EM), revealing three different neutralizing epitopes and a shared neutralization mechanism among three representative neutralizing monoclonal antibodies. The results highlight HPV58 genotype specificity and broaden our understanding of HPV58 neutralization sites for antiviral research.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Blandine Noailly, Melyssa Yaugel-Novoa, Justine Werquin, Fabienne Jospin, Daniel Drocourt, Thomas Bourlet, Nicolas Rochereau, Stephane Paul
Summary: Broadly neutralizing antibodies (bNAbs) show promising potential for HIV-1 prevention. Different bNAb isotypes have varying abilities in viral neutralization and ADCC-like activity. These findings contribute to the search for new treatments and antibody-based vaccines.
Article
Microbiology
Kasopefoluwa Y. Oguntuyo, Christian S. Stevens, Chuan-Tien Hung, Satoshi Ikegame, Joshua A. Acklin, Shreyas S. Kowdle, Jillian C. Carmichael, Hsin-Ping Chiu, Kristopher D. Azarm, Griffin D. Haas, Fatima Amanat, Jeromine Klingler, Ian Baine, Suzanne Arinsburg, Juan C. Bandres, Mohammed N. A. Siddiquey, Robert M. Schilke, Matthew D. Woolard, Hongbo Zhang, Andrew J. Duty, Thomas A. Kraus, Thomas M. Moran, Domenico Tortorella, Jean K. Lim, Andrea Gamarnik, Catarina E. Hioe, Susan Zolla-Pazner, Stanimir S. Ivanov, Jeremy P. Kamil, Florian Krammer, Benhur Lee
Summary: The global COVID-19 pandemic has led to the development of a standardized virus neutralization assay using pseudotyped particles for efficient and accurate testing of neutralizing antibodies against SARS-CoV-2. The assay showed strong positive correlation with live-virus neutralizations in confirmed convalescent-patient sera and can be used for meaningful comparisons across diverse cohorts. This standardized assay is necessary for assessing the efficacy of various vaccines and antibody-based therapeutics.
Article
Virology
Jim Boonyaratanakornkit, Anton M. Sholukh, Matthew Gray, Emily L. Bossard, Emily S. Ford, Kizzmekia S. Corbett, Lawrence Corey, Justin J. Taylor
Summary: The human Betacoronavirus OC43 is a common cause of respiratory viral infections in adults and children, with lung infections associated with mortality. A human monoclonal antibody OC2 was isolated that neutralizes live OC43 virus. Two in vitro assays using cytopathic effect and ELISA were developed to measure neutralizing activity of OC2 and human plasma, which could be useful for studying humoral responses to OC43 and cross-neutralization with other important betacoronaviruses.
Article
Immunology
Jose Javier Morales-Nunez, Jose Francisco Munoz-Valle, Paola Carolina Torres-Hernandez, Jorge Hernandez-Bello
Summary: This review article discusses the essential concepts of NAbs, including their mechanisms of action, production, and detection techniques, as well as providing an overview of the clinical use of antibodies in COVID-19.
Article
Microbiology
Sabine Lichtenegger, Sabine Saiger, Melina Hardt, Susanne Kulnik, Gabriel E. Wagner, Barbara Kleinhappl, Karoline Assig, Andrea Zauner, Michelle Ober, Janine Kimpel, Dorothee von Laer, Kurt Zatloukal, Ivo Steinmetz
Summary: Measurement of SARS-CoV-2 neutralizing antibodies is crucial for determining immunity against newly emerging variants. This study presents a faster live virus assay that detects neutralizing antibodies through early measurement of intracellular virus reduction by SARS-CoV-2 qRT-PCR. The assay showed high sensitivity and specificity, with a strong correlation to conventional virus neutralization assays, indicating its potential for rapidly determining immunity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Psychology, Multidisciplinary
Ying Jiang
Summary: This study investigated the effects of L1-L2 congruency, collocation type, and restriction on L2 collocational processing. The results revealed that non-native speakers were sensitive to L1-L2 congruency, while native speakers were sensitive to collocation restriction.
FRONTIERS IN PSYCHOLOGY
(2022)
Article
Immunology
Lydia Riepler, Annika Roessler, Albert Falch, Andre Volland, Wegene Borena, Dorothee von Laer, Janine Kimpel
Summary: Neutralizing antibodies are crucial for protection against viruses like SARS-CoV-2. This study compared four different assays for detecting these antibodies and found that they were all robust and yielded comparable results, indicating their reliability.
Article
Virology
Chun-Yu Chen, Kuan-Ting Liu, Shin-Ru Shih, Jung- Ye, Yih-Ting Chen, Cheng-Kai Hsu, Heng-Chih Pan, Heng-Jung Hsu, Chiao-Yin Sun, Chin-Chan Lee, Chun-Ying Wu, Chi-Chun Lai, I-Wen Wu
Summary: This study found that neutralizing antibody levels were lower in hemodialysis patients compared to healthy controls after receiving the ChAdOx1 vaccine, and dialysis adequacy could predict seroconversion in these patients.
Article
Immunology
Arati Mane, Sanket Limaye, Linata Patil, Urmila Kulkarni-Kale
Summary: This study analyzed the L2 variations of 148 cervical HPV-16 isolates from Indian women and found substitutions that were significantly associated with high-grade cervical neoplastic status. Multiple substitutions were observed for the first time in Indian isolates. These findings have important implications for cervical cancer oncogenesis and the design of next-generation L2-based HPV vaccines.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2022)
Article
Microbiology
Anna Godi, Stuti Vaghadia, Clementina Cocuzza, Elizabeth Miller, Simon Beddows
Summary: The major virus coat protein of human papillomavirus type 16 (HPV16) is targeted by antibodies induced by both natural infection and vaccination. The study investigates the contribution of surface-exposed loops of the major capsid protein to the antigenic domains recognized by these antibodies. The results suggest that vaccine-induced antibodies target a different area compared to natural infection antibodies, providing a better understanding of HPV16-specific immunity.
MICROBIOLOGY SPECTRUM
(2022)