Article
Biochemistry & Molecular Biology
George R. Young, Aaron K. W. Ferron, Veera Panova, Urszula Eksmond, Peter L. Oliver, George Kassiotis, Jonathan P. Stoye
Summary: Inbred mice genomes contain around 50 endogenous murine leukemia virus (MLV) loci, with the Gv1 locus being important for controlling MLV transcription and cell-surface presentation of the G(IX) antigen. Zfp998 gene is identified as Gv1 and is essential for determining the G(IX)(+) phenotype, highlighting its role in epigenetic suppression of endogenous MLVs and control of endogenous retroviruses.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Computer Science, Software Engineering
Madhavi Latha Yadav Bangaru, Ravi Kumar Medabalimi, Sobhan Babu, Nidhanapati K. Raghavendra
Summary: Silent mutations allowing for restriction site modification can aid in screening successful mutants during site-directed mutagenesis. However, introducing a new restriction site requires the analysis of degenerate sequences within mutant primers, which becomes increasingly laborious, time-consuming, and prone to errors as the number of degenerate codons increases. A software named 'REMP' was developed to instantly generate degenerate sequences with restriction sites that are 6-8 base pairs in length, allowing users to edit the list of restriction sites without the need for writing computer code or knowing a program language.
Article
Microbiology
Yudi Zhang, Volker Kinast, Julie Sheldon, Nicola Frericks, Daniel Todt, Matthias Zimmer, Neva Caliskan, Richard J. P. Brown, Eike Steinmann, Thomas Pietschmann
Summary: Mice are resistant to infection with human-tropic hepatitis C virus (HCV), but a related rodent hepatitis C virus (RHV) can circulate in wild rodents. This study found that the Shiftless (Shfl) gene is expressed in humans and rodents, and it restricts HCV and RHV replication by inhibiting viral RNA replication. These findings are important for understanding viral host adaptation and developing animal models.
MICROBIOLOGY SPECTRUM
(2023)
Review
Oncology
Kristen J. Kurtz, Shannon E. Conneely, Madeleine O'Keefe, Katharina Wohlan, Rachel E. Rau
Summary: Acute myeloid leukemia (AML) is a genetically and phenotypically heterogeneous hematologic malignancy. Murine models of AML are indispensable research tools to better understand the mechanisms and test novel therapeutic approaches for this disease.
FRONTIERS IN ONCOLOGY
(2022)
Article
Microbiology
Supawadee Umthong, Brian Lynch, Uddhav Timilsina, Brandon Waxman, Emily B. Ivey, Spyridon Stavrou
Summary: This study explores the antiviral mechanism of MARCH proteins, which act as antiretroviral factors by reducing the incorporation of envelope glycoproteins in budding virions. A comprehensive analysis reveals that MARCH proteins have broad antiviral functions, restricting envelope glycoproteins from various viruses to different degrees. Comparisons between human and mouse MARCH genes show differences in the mechanism of restriction among MARCH proteins.
Article
Biology
Kaushik Inamdar, Feng-Ching Tsai, Rayane Dibsy, Aurore de Poret, John Manzi, Peggy Merida, Remi Muller, Pekka Lappalainen, Philippe Roingeard, Johnson Mak, Patricia Bassereau, Cyril Favard, Delphine Muriaux
Summary: The cellular I-BAR protein IRSp53 has been identified as a crucial factor in HIV-1 membrane curvature during particle formation, showing a strong interplay with the retroviral Gag protein. IRSp53 is required for the progression of HIV-1 membrane curvature and its presence is observed in purified HIV-1 particles. The preferential localization of IRSp53 around HIV-1 Gag assembly sites highlights its importance in full HIV-1 particle assembly.
Article
Biochemistry & Molecular Biology
Federico Giovannoni, Cecilia A. Vazquez, Pablo Thomas, Ricardo M. Gomez, Cybele C. Garcia
Summary: This study reveals that the interaction between the New World mammarenavirus Junin (JUNV) and the antiviral protein PML is different from that of the Old World mammarenavirus lymphocytic choriomeningitis virus (LCMV). It also demonstrates that the mechanism of JUNV restriction in human cells is distinct from LCMV.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Katarzyna M. Sitnik, Fran Krstanovic, Natascha Goedecke, Ulfert Rand, Tobias Kubsch, Henrike Maass, Yeonsu Kim, Ilija Brizic, Luka Cicin-Sain
Summary: To date, no herpesvirus has been found to persist in fibroblastic cells, but this study reveals that murine cytomegalovirus can persist in PDGFR alpha-positive fibroblastic cells for a long time and across organs. These cells not only support latent infection but also productive virus replication, and the presence of STAT1 is required for the virus to persist in vivo. This study highlights the dual role of fibroblastic cells as both a reservoir for latent virus and a site for replication.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Vinicius Pinho dos Reis, Markus Keller, Katja Schmidt, Rainer Guenter Ulrich, Martin Hermann Groschup
Summary: The absence of alpha V beta 3 integrin significantly impairs the replication of flaviviruses.
Article
Biochemistry & Molecular Biology
William A. Nyberg, Jonathan Ark, Angela To, Sylvanie Clouden, Gabriella Reeder, Joseph J. Muldoon, Jing-Yi Chung, William H. Xie, Vincent Allain, Zachary Steinhart, Christopher Chang, Alexis Talbot, Sandy Kim, Alan Rosales, L. Patrick Havlik, Harold Pimentel, Aravind Asokan, Justin Eyquem
Summary: The precise targeting of large transgenes to T cells using homology-directed repair has been significant for adoptive cell therapies and T cell biology. The use of adeno-associated virus (AAV) for delivery of DNA templates has improved knockin efficiencies, but current AAV serotypes have limited tropism in human T cells used in immunodeficient mouse models. To overcome this, Ark313, a synthetic AAV that exhibits high transduction efficiency in murine T cells, was developed. Ark313 enables targeted knockins in murine T cells, allowing for preclinical modeling of Trac-targeted CAR-T and transgenic TCR-T cells in immunocompetent models. Efficient gene targeting in murine T cells holds great potential for improved cell therapies and advances in experimental T cell immunology.
Article
Multidisciplinary Sciences
Julia Su Zhou Li, Ammal Abbasi, Dong Hyun Kim, Scott M. Lippman, Ludmil B. Alexandrov, Don W. Cleveland
Summary: Epstein-Barr virus (EBV) is an oncogenic herpesvirus associated with various cancers. In this study, researchers found that the EBNA1 protein of EBV binds to a repetitive copy of an 18-base-pair imperfect palindromic sequence on human chromosome 11q23, leading to chromosome breakage. Increasing levels of EBNA1 binding trigger chromosome breakage and acquisition of structural variations in chromosome 11, which is associated with cancer development. The presence of EBV is also associated with chromosome rearrangements in various cancer types.
Article
Virology
Yue Zheng, Erin T. Larragoite, Elizabeth S. C. P. Williams, Juan Lama, Isabel Cisneros, Julio C. Delgado, Patricia Slev, Jenna Rychert, Emily A. Innis, Mayte Coiras, Matthew T. Rondina, Adam M. Spivak, Vicente Planelles
Summary: The study developed pseudovirion particles for quick measurement of neutralizing antibody titers under biocontainment level 2 laboratory conditions, showing promise for assessing immunity in infected patients and candidate vaccines. The results demonstrated high concordance between the pseudovirion assay and the Siemens SARS-CoV-2 IgG test in evaluating neutralizing activity.
Article
Biochemical Research Methods
Wolfram Volkwein, Melanie Pavlovic, Martina Anton, Maren Haase, Thorsten Stellberger, Amin Jarrar, Ulrich Busch, Armin Baiker
Summary: The method described here uses a quantitative real-time PCR with hydrolysis probe to detect MLV/MSCV genomic RNA efficiently, enabling fast and accurate transduction experiments and research in gene delivery.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Virology
Elliott S. Chiu, Coby A. McDonald, Sue VandeWoude
Summary: Endogenous retroviruses (ERVs) have significant biological impacts on host cell function and susceptibility to infectious agents, particularly in interactions with exogenous retroviral progenitors (XRVs). The transcription of endogenous FeLV (enFeLV) in cats is associated with lower susceptibility to exogenous FeLV infections, with lymphoid-derived tissues showing higher levels of enFeLV transcripts. The presence of microRNA transcription in tissues important for FeLV infection suggests potential impacts on domestic cat susceptibility and pathogenesis.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Mizuki Yamamoto, Jin Gohda, Ayako Kobayashi, Keiko Tomita, Youko Hirayama, Naohiko Koshikawa, Motoharu Seiki, Kentaro Semba, Tetsu Akiyama, Yasushi Kawaguchi, Jun-ichiro Inoue
Summary: The study found that the cell surface entry pathway of SARS-CoV-2 includes a metalloproteinase-mediated pathway in addition to the TMPRSS2 and endosomal pathways. The metalloproteinase-mediated pathway requires prior cleavage of the spike protein into two domains, a condition met by SARS-CoV-2 but not by the related human coronavirus SARS-CoV. Additionally, inhibiting metalloproteins was crucial in preventing cell death, which may lead to organ damage. The findings provide new insights into the unique pathogenesis of COVID-19 and are relevant to the development of effective therapies.
Article
Virology
Gary Z. Wang, Stephen P. Goff
JOURNAL OF VIROLOGY
(2017)
Editorial Material
Multidisciplinary Sciences
Stephen P. Goff
Article
Biochemistry & Molecular Biology
Massilva Rahmoun, Rowena Lavery, Sabine Laurent-Chaballier, Nicolas Bellora, Gayle K. Philip, Moira Rossitto, Aleisha Symon, Eric Pailhoux, Florence Cammas, Jessica Chung, Stefan Bagheri-Fam, Mark Murphy, Vivian Bardwell, David Zarkower, Brigitte Boizet-Bonhoure, Philippe Clair, Vincent R. Harley, Francis Poulat
NUCLEIC ACIDS RESEARCH
(2017)
Article
Virology
Franziska K. Geis, Melanie Galla, Dirk Hoffmann, Johannes Kuehle, Daniela Zychlinski, Tobias Maetzig, Juliane W. Schott, Adrian Schwarzer, Christine Goffinet, Stephen P. Goff, Axel Schambach
Article
Multidisciplinary Sciences
Oya Cingoz, Stephen P. Goff
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Michael J. Metzger, Ashley N. Paynter, Mark E. Siddall, Stephen P. Goff
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Virology
Andreia Lee, Oya CingOz, Yosef Sabo, Stephen P. Goff
Article
Multidisciplinary Sciences
Yiping Zhu, Gary Z. Wang, Oya Cingoz, Stephen P. Goff
Article
Biochemistry & Molecular Biology
Xinlu Wang, Yifang Xuan, Yuling Han, Xiang Ding, Kai Ye, Fuquan Yang, Pu Gao, Stephen P. Goff, Guangxia Gao
Article
Microbiology
Oya Cingoz, Stephen P. Goff
Article
Biochemistry & Molecular Biology
Maicol Mancini, Laura Papon, Alain Mange, Florence Cammas, Eric Fabbrizio
BIOCHEMICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Nehme Saksouk, Shefqet Hajdari, Yannick Perez, Marine Pratlong, Celia Barrachina, Celine Graber, Damien Gregoire, Aliki Zavoriti, Amelie Sarrazin, Nelly Pirot, Jean-Yohan Noel, Lakhdar Khellaf, Eric Fabbrizio, Eric Julien, Florence M. Cammas
Article
Multidisciplinary Sciences
Moira Rossitto, Stephanie Dejardin, Chris M. Rands, Stephanie Le Gras, Roberta Migale, Mahmoud-Reza Rafiee, Yasmine Neirijnck, Alain Pruvost, Anvi Laetitia Nguyen, Guillaume Bossis, Florence Cammas, Lionel Le Gallic, Dagmar Wilhelm, Robin Lovell-Badge, Brigitte Boizet-Bonhoure, Serge Nef, Francis Poulat
Summary: Gonadal fate in mammals is determined during embryogenesis and is actively maintained in adulthood. This study shows that E3-SUMO ligase activity of TRIM28 is required for ovarian identity maintenance and testicular-specific gene repression in mouse adult ovary; in its absence, ovarian granulosa cells transdifferentiate to Sertoli cells.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Sebastien Calvet, Sephora Sallis, Nehme Saksouk, Cosette Rebouissou, Catherine Teyssier, Annick Lesne, Florence Cammas, Thierry Forne
Summary: About half of the mammalian genome is made up of repeated elements, and endogenous retroviruses (ERVs) are known to affect gene expression and cancer development. The HP1 protein has a crucial role in establishing and functioning heterochromatin, and its loss in hepatocytes results in the reactivation of specific ERVs and the development of liver tumors. This study discovered that certain HP1-dependent ERVs located upstream of genes Mbd1 and Trim24 can act as alternative promoters or potential enhancers, forming a loop with promoters of endogenous genes depending on the genomic context and HP1 expression level.
Article
Microbiology
Yiping Zhu, Shukun Luo, Yosef Sabo, Cheng Wang, Liang Tong, Stephen P. Goff
CELL HOST & MICROBE
(2017)
