Article
Chemistry, Multidisciplinary
Ayesha M. Patel, E. Neil G. Marsh
Summary: The study demonstrates that viperin activates a component of the protein ubiquitination machinery, enhancing TRAF6's ubiquitin transfer activity and increasing important polyubiquitinated forms, suggesting a potential role as an agonist of immune signaling pathways.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Immunology
Kumar Saurabh Srivastava, Vandana Jeswani, Nabanita Pal, Babita Bohra, Vaishali Vishwakarma, Atharva Ashish Bapat, Yamini Prashanti Patnaik, Navin Khanna, Rahul Shukla
Summary: Japanese encephalitis virus (JEV) is a potentially severe brain infection that spreads through mosquito bites. Currently, there is no licensed anti-JEV drug available, although there are a few licensed vaccines with limited global use. With over 67,000 cases of JE annually, there is an urgent need to find suitable antiviral drugs for treatment.
Article
Virology
Jeong-Min Hong, Ali Newaz Munna, Ji-Hong Moon, Jong-Hoon Kim, Jae-Won Seol, Seong-Kug Eo, Sang-Youel Park
Summary: This study investigated the role of PrPc in regulating autophagy flux and its potential antiviral activity against Japanese encephalitis virus (JEV) infection. The results demonstrated that PrPc effectively inhibited JEV propagation by regulating autophagy flux, offering a promising therapeutic target for flavivirus infection.
Article
Biochemistry & Molecular Biology
Cornelia E. Zorca, Armaan Fallahi, Sophie Luo, Mohamed A. Eldeeb
Summary: Selective protein degradation is crucial for maintaining cellular homeostasis and is disrupted in various diseases. Targeted protein degradation approaches, based on existing cellular mechanisms, offer promising therapeutic strategies for regulating protein levels effectively.
Article
Biochemistry & Molecular Biology
Amjad Ali, Vivek Kumar, Akhil C. Banerjea
Summary: HIV-1 accessory protein Vif neutralizes the cellular restriction factor APOBEC3G through ubiquitination and degradation, which is regulated by the E3 ubiquitin ligase CHIP. Increased CHIP expression leads to reduced Vif levels and destabilization of APOBEC3G, confirming CHIP's role as a negative regulator of Vif.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Kyung Mi Choi, Jeong Jin Kim, Jihye Yoo, Ku Sul Kim, Youngeun Gu, John Eom, Haengdueng Jeong, Kyungeun Kim, Ki Taek Nam, Young Soo Park, Joon-Yong Chung, Jun-Young Seo
Summary: This study reveals the role of interferon-inducible protein viperin in driving metabolic alterations in cancer cells. Viperin promotes lipogenesis and glycolysis by inhibiting fatty acid beta-oxidation in cancer cells. It is primarily expressed in cancer stem-like cells and contributes to tumor growth and progression. This finding provides new insights into the metabolic reprogramming of cancer cells.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Ekachai Dumkliang, Boonnada Pamornpathomkul, Prasopchai Patrojanasophon, Tanasait Ngawhirunpat, Theerasak Rojanarata, Sutee Yoksan, Praneet Opanasopit
Summary: The study evaluated the immunization effect of mucoadhesive nanoparticles loaded with live attenuated Japanese encephalitis-chimeric virus vaccine through the intranasal route, demonstrating that intranasal immunization stimulated immune protection and enhanced mucosal immune response.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Xinying Zhou, Zelin Zhang, Hui Xu, Bo Zhu, Lijie Zhang, Linmiao Lie, Yingqi Huang, Xialin Du, Honglin Liu, Yanfen Li, Yulan Huang, Shengfeng Hu, Chaoying Zhou, Qian Wen, Mailkel P. Pepplenbosch, Li Ma
Summary: Viperin protein is increased in abundance in patients with lymphatic and pulmonary tuberculosis (TB), and its deficiency reduces mycobacterium tuberculosis bacterial loads and enhances macrophage responses. Viperin promotes Mtb infection by inhibiting host innate immune responses in macrophages.
Article
Immunology
Cheng-Yi Chang, Chih-Cheng Wu, Jiaan-Der Wang, Jian-Ri Li, Ya-Yu Wang, Shih-Yi Lin, Wen-Ying Chen, Su-Lan Liao, Chun-Jung Chen
Summary: The study demonstrated that DHA exhibits anti-inflammatory, neuroprotective, and antiviral effects against JEV infection by modulating the immune response of microglia and neurons. This suggests that DHA may be a potential candidate for treating neuroinflammation associated with viral infections such as Japanese Encephalitis.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Chemistry, Multidisciplinary
Kate A. Kragness, Darci J. Trader
Summary: The proteasome plays a crucial role in maintaining cellular homeostasis by degrading proteins. It can target proteins for degradation through two different pathways, the ubiquitin-dependent and ubiquitin-independent pathways. Age-related protein accumulation and aggregation can occur due to decreased proteasome activity, leading to increased cellular stress. Increasing proteasome activity has shown potential as a therapeutic strategy to mitigate cellular stress caused by age-related protein accumulation.
ISRAEL JOURNAL OF CHEMISTRY
(2023)
Article
Immunology
Zhongbing Qi, Jing Zhao, Yuhua Li, Bin Zhang, Shichuan Hu, Yanwei Chen, Jinhu Ma, Yongheng Shu, Yunmeng Wang, Ping Cheng
Summary: In this study, the researchers discovered that JEV-LAV had oncolytic activity against GBMs, inhibiting their growth in vivo. It also increased CD8+ T cell infiltration in tumor tissues and remodeled the immunosuppressive GBM microenvironment, improving the response of aPD-L1 blockade therapy. The safety of JEV-LAV intracerebral injection in animals supported its potential for clinical use in GBM treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Merav D. Shmueli, Daoud Sheban, Avital Eisenberg-Lerner, Yifat Merbl
Summary: Histones are key protein building blocks of chromatin, regulated by intricate mechanisms to control chromatin compaction and epigenetic regulation. Proteasome-dependent degradation, along with protein modifications, play roles in regulating histone stability, impacting cellular and physiological states.
Article
Immunology
Mya Myat Ngwe Tun, Aung Kyaw Kyaw, Khine Mya Nwe, Shingo Inoue, Kyaw Zin Thant, Kouichi Morita
Summary: A study conducted among monastic school children in Mandalay, Myanmar showed that 87% of students still had neutralizing antibodies against JEV six months after receiving the SA-14-14-2 live-attenuated vaccine. Antibody titers increased with age, with statistically significant differences noted across age groups.
Article
Cell Biology
Kartikeya Vijayasimha, Marilyn Vo Tran, Amy L. Leestemaker-Palmer, Brian P. Dolan
Summary: The study demonstrates that fusion of NEDD8 to a protein of interest leads to rapid degradation, depending on functional ubiquitin-conjugation system. However, the inhibitor of the E1 activating enzyme for NEDD8 failed to prevent degradation of other destabilized substrates.
Article
Multidisciplinary Sciences
Afu Fu, Victoria Cohen-Kaplan, Noa Avni, Ido Livneh, Aaron Ciechanover
Summary: The degradation of proteins through the ubiquitin-proteasome system is a complex multistep process that relies on the coordinated activity of various enzymes. Nuclear condensates containing essential components like p62 play a crucial role in protein quality control and degradation, especially under stress conditions. These assemblies, generated through liquid-liquid phase separation, efficiently facilitate proteolysis of nuclear proteins and unassembled proteasome subunits, indicating their involvement in cellular protein quality control.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Hsin- Wang, Zih-Shiuan Chuang, Yu-Ting Kao, Yi-Ling Lin, Jian-Jong Liang, Chun-Che Liao, Ching-Len Liao, Michael M. C. Lai, Chia-Yi Yu
Summary: SARS-CoV-2 pseudovirus coated with SEM increases infectivity by promoting S protein priming, binds efficiently to cells expressing hACE2, and can be used to evaluate infectivity and neutralization capability of dominant S variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Kai-Che Wei, Shih-Fan Lai, Wei-Lun Huang, Kuo-Chung Yang, Ping-Chin Lai, Wan-Ju Wei, Tsung-Hsien Chang, Yun-Chen Huang, Ya-Chuan Tsai, Shin-Chih Lin, Sun-Jang Lin, Shih-Chieh Lin
Summary: YAP1 overexpression in skin specimens from FICRD patients plays a protective role by promoting DNA damage repair and inhibiting fibrosis. Targeting YAP1 downstream genes like NR3C1, which encodes glucocorticoid receptor, has therapeutic potential for FICRD patients. Investigating the underlying mechanism of FICRD is crucial for developing effective treatments.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Immunology
Jer-Hwa Chang, Jeng-Fong Chiou, Ching-Sheng Hung, Ming-Che Liu, Hui-Wen Chang, Shiao-Ya Hong, Cheng-Yi Wang, Yi-Ling Lin, Yi-Chen Hsieh, Chi-Li Chung, Ying-Shih Su, Shu-Tai Shen Hsiao, Doresses Liu, Jian-Jong Liang, Chun-Che Liao, Chih-Shin Chang, Kevin Shu-Leung Lai, Han-Chuan Chuang, Ko-Ling Chien, Wei-Ciao Wu, Yuan-Chii G. Lee, Sey-En Lin, Yung-Kang Shen, Chiung-Fang Hsu, Jude Chu-Chun Wang, Shih-Hsin Hsiao
Summary: The ChAdOx1 nCoV-19 vaccine elicits a robust humoral immune response against SARS-CoV-2 in Taiwanese recipients after standard prime-boost vaccination. The vaccine is well-tolerated, and antibody levels are associated with factors such as age, gender, and body weight index.
Article
Cell Biology
Ching Ying Huang, Martin W. Nicholson, Jyun Yuan Wang, Chien Yu Ting, Ming Heng Tsai, Yu Che Cheng, Chun Lin Liu, Darien Z. H. Chan, Yi Chan Lee, Ching Chuan Hsu, Yu Hung Hsu, Chiou Fong Yang, Cindy M. C. Chang, Shu Chian Ruan, Po Ju Lin, Jen Hao Lin, Li Lun Chen, Marvin L. Hsieh, Yuan Yuan Cheng, Wan Tseng Hsu, Yi Ling Lin, Chien Hsiun Chen, Yu Hsiang Hsu, Ying Ta Wu, Timothy A. Hacker, Joseph C. Wu, Timothy J. Kamp, Patrick C. H. Hsieh
Summary: In this study, a population-based hiPSC drug screening platform is established to assess toxicity. Thirteen HLA-homozygous super donors are identified after recruiting 1,000 healthy donors and screening for high-frequency HLA haplotypes, representing at least 477,611,135 of the global population. The differentiated hiPSCs into cardiomyocytes and neurons demonstrate their utility in a high-throughput toxicity screen. Dose-dependent toxicity and functional modulation of hit compounds are validated, and reproducible in vivo drug toxicity results using mouse models are shown. This study shows the feasibility of using a population-based hiPSC drug screening platform to study inter population differences in drug toxicity and adverse drug reactions.
Article
Chemistry, Applied
Hsiao-Ting Chen, Chi-Wen Chuang, Ju-Chien Cheng, Yung-Ju Yeh, Tsung-Hsien Chang, Yu-Ting Shi, Chih-Hua Chao
Summary: Two rearranged terpenoids with a rare moiety and a megastigmane were isolated from the leaves of Euphorbia leucocephala. In addition, three known compounds were also identified. These compounds showed potent anti-influenza A virus activity.
NATURAL PRODUCT RESEARCH
(2023)
Article
Virology
Ming-Wei Jan, Chih-Yun Chiu, Jih-Jung Chen, Tsung-Hsien Chang, Kuen-Jer Tsai
Summary: This study found that the infection of Parechovirus type-A3 (PeV-A3) was reduced in human platelet lysate (hPL)-cultured cells compared to fetal bovine serum (FBS)-cultured cells. Mechanistic analysis revealed that hPL stimulates the type I interferon (IFN) antiviral pathway, resulting in the activation of related transcription factors and the expression of antiviral genes. Enhanced PeV-A3 replication was observed in hPL-cultured cells treated with STAT-1 inhibitor and STAT1 shRNA. These findings suggest that hPL may have potential antiviral effects.
Article
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Summary: Ciclesonide blocks coronavirus-induced cytokine production by increasing I kappa B alpha protein levels and decreasing p65 nuclear translocation, and it can be used in the treatment of COVID-19 patients. The combination of Ciclesonide and dbq33b synergistically decreases cytokine levels and inhibits coronavirus replication. Therefore, this combination therapy is a promising treatment option for COVID-19 patients with high viral loads and NF-kappa B-mediated cytokine storms.
Article
Chemistry, Medicinal
Martin W. Nicholson, Ching-Ying Huang, Jyun-Yuan Wang, Chien-Yu Ting, Yu-Che Cheng, Darien Z. H. Chan, Yi-Chan Lee, Ching-Chuan Hsu, Yu-Hung Hsu, Cindy M. C. Chang, Marvin L. Hsieh, Yuan-Yuan Cheng, Yi-Ling Lin, Chien-Hsiun Chen, Ying-Ta Wu, Timothy A. Hacker, Joseph C. Wu, Timothy J. Kamp, Patrick C. H. Hsieh
Summary: This study utilized a drug screening platform based on human induced pluripotent stem cells to evaluate the cardiotoxicity and neurotoxicity of anti-COVID-19 drugs. The researchers found that certain drugs displayed cardiotoxic or neurotoxic effects, providing valuable information for clinicians when choosing treatment options.
Article
Biology
Wei-Chun Tang, Yen-Ting Liu, Cheng-Han Yeh, Chieh-Han Lu, Chiao-Hui Tu, Yi-Ling Lin, Yu-Chun Lin, Tsui-Ling Hsu, Liang Gao, Shu-Wei Chang, Peilin Chen, Bi-Chang Chen
Summary: This study improves lattice lightsheet microscopy (LLSM) to manipulate cellular behavior with subcellular resolution through optogenetic activation (optoLLSM). By integrating a position-controllable Bessel beam as a stimulation source, spatiotemporal photoactivation can be achieved by changing spatial light modulator (SLM) patterns. The study demonstrates the ability to control membrane ruffling and cell migration behavior using light-triggered recruitment of phosphoinositide 3-kinase and guided cell migration with low energy power.
COMMUNICATIONS BIOLOGY
(2022)
Correction
Pharmacology & Pharmacy
Yue-Zhi Lee, Hsing-Yu Hsu, Cheng-Wei Yang, Yi-Ling Lin, Sui-Yuan Chang, Ruey-Bing Yang, Jian-Jong Liang, Tai-Ling Chao, Chun-Che Liao, Han-Chieh Kao, Jang-Yang Chang, Huey-Kang Sytwu, Chiung-Tong Chen, Shiow-Ju Lee
Article
Microbiology
Hui-Ying Ko, Yao-Tsun Li, Ya-Yuan Li, Ming-Tsai Chiang, Yi-Ling Lee, Wen-Chun Liu, Chun-Che Liao, Chih-Shin Chang, Yi-Ling Lin
Summary: COVID-19 is a major global public health threat, and RT-qPCR is used for viral RNA detection. Saliva has been suggested as a practical alternative to nasopharyngeal swabs for viral RNA detection. This study evaluated the effect of saliva on viral RNA detection and found that saliva increased the C-T values of SARS-CoV-2 RNA samples compared to viral transport medium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
I-Jung Lee, Cheng-Pu Sun, Ping-Yi Wu, Yu-Hua Lan, I-Hsuan Wang, Wen-Chun Liu, Joyce Pei-Yi Yuan, Yu-Wei Chang, Sheng-Che Tseng, Szu- Tsung, Yu-Chi Chou, Monika Kumari, Yin-Shiou Lin, Hui-Feng Chen, Tsung-Yen Chen, Chih-Chao Lin, Chi-Wen Chiu, Chung-Hsuan Hsieh, Cheng-Ying Chuang, Chao-Min Cheng, Hsiu-Ting Lin, Wan-Yu Chen, Fu-Fei Hsu, Ming-Hsiang Hong, Chun-Che Liao, Chih-Shin Chang, Jian-Jong Liang, Hsiu-Hua Ma, Ming-Tsai Chiang, Hsin-Ni Liao, Hui-Ying Ko, Liang-Yu Chen, Yi-An Ko, Pei-Yu Yu, Tzu-Jing Yang, Po-Cheng Chiang, Shang-Te Hsu, Yi-Ling Lin, Chong-Chou Lee, Han-Chung Wu, Mi-Hua Tao
Summary: This study reports an mRNA-based vaccine using an engineered hybrid receptor binding domain that can generate neutralizing antibodies and T cell responses against various SARS-CoV-2 variants. The results demonstrate that including different antigenic mutations from different variants is a feasible approach to develop vaccines with broad protection.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Chemistry, Medicinal
Hui-Ming Chen, Chien-Ming Huang, Li-Chai Chen, Chang-Syun Yang, Tsung-Hsien Chang, Chun-Lin Chen, Ping-Jyun Sung, Wen-Lung Kuo, Ming-Jen Cheng, Jen-Wen Hsiao, Jih-Jung Chen
Summary: A new triterpenoid compound was isolated from the leaves of Eriobotrya deflexa f. deflexa, and its structure was determined. This compound, along with other known compounds, exhibited inhibitory effects on LPS-induced NO generation.
CHEMISTRY OF NATURAL COMPOUNDS
(2022)
Article
Medicine, Research & Experimental
Chang Yi Wang, Kao-Pin Hwang, Hui-Kai Kuo, Wen-Jiun Peng, Yea-Huei Shen, Be-Sheng Kuo, Juin-Hua Huang, Hope Liu, Yu-Hsin Ho, Feng Lin, Shuang Ding, Zhi Liu, Huan-Ting Wu, Ching-Tai Huang, Yuarn-Jang Lee, Ming-Che Liu, Yi-Ching Yang, Po-Liang Lu, Hung-Chin Tsai, Chen-Hsiang Lee, Zhi-Yuan Shi, Chun-Eng Liu, Chun-Hsing Liao, Feng-Yee Chang, Hsiang-Cheng Chen, Fu-Der Wang, Kuo-Liang Hou, Jennifer Cheng, Min-Sheng Wang, Ya-Ting Yang, Han-Chen Chiu, Ming-Han Jiang, Hao-Yu Shih, Hsuan-Yu Shen, Po-Yen Chang, Yu-Rou Lan, Chi-Tian Chen, Yi-Ling Lin, Jian-Jong Liang, Chun-Che Liao, Yu-Chi Chou, Mary Kate Morris, Carl V. Hanson, Farshad Guirakhoo, Michael Hellerstein, Hui-Jing Yu, Chwan-Chuen King, Tracy Kemp, D. Gray Heppner, Thomas P. Monath
Summary: The UB-612 vaccine showed favorable safety profile, long-lasting antibody response, and broad T cell immunity against SARS-CoV-2 variants, including Delta and Omicron, in phase I/II trials.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Virology
Han Chiu, Hsin-Ping Chiu, Han-Pang Yu, Li-Hsiung Lin, Zih-Ping Chen, Yi-Ling Lin, Ren-Jye Lin
Summary: ZFP36L1 acts as a host innate defender against flaviviruses by targeting and degrading viral RNA. It destabilizes the viral genome by recruiting cellular mRNA decay machineries. The specific recognition of AU-rich elements within viral RNA by ZFP36L1 triggers the degradation of viral RNA transcripts.
JOURNAL OF VIROLOGY
(2022)