Review
Cell Biology
Raphael Gaudin, Jacky G. Goetz
Summary: The dissemination and replication of viruses within hosts is a complex multistep process where viruses can reach tissues distant from their point of entry by subverting subcellular mechanisms. Modeling these steps in appropriate resolution and within animal models is challenging, and mimicking them in vitro fails to replicate the complexity of the cellular ecosystem. The zebrafish model provides a transparent window to study new cellular mechanisms of viral dissemination in vivo.
TRENDS IN CELL BIOLOGY
(2021)
Review
Multidisciplinary Sciences
Rafael Borges Rosa, Emilene Ferreira de Castro, Murilo Vieira da Silva, Denise Caroline Paiva Ferreira, Ana Carolina Gomes Jardim, Igor Andrade Santos, Mikaela dos Santos Marinho, Flavia Batista Ferreira Franca, Lindomar Jose Pena
Summary: The emergence and rapid spread of monkeypox virus (MPXV) outside of endemic areas during the COVID-19 pandemic has created a global health emergency. Scientists have been working on developing in vitro and in vivo models for MPXV research. In vitro models, including 2D and 3D cultures, are cost-effective and can be controlled, but may not fully represent physiological conditions. Therefore, in vivo models are being characterized to meet the demand for studying MPXV. This review summarizes the existing MPXV models and discusses their contributions to understanding pathogenesis, replication, transmission, and antiviral treatment.
Article
Immunology
Ulrike S. Diesterbeck, Henrike P. Ahsendorf, Andre Frenzel, Ahmad Reza Sharifi, Thomas Schirrmann, Claus-Peter Czerny
Summary: A panel of potent neutralizing antibodies offers protection against orthopoxvirus infections. In an in vivo experiment, purified scFv-1.2.2.H9 and IgG1-1.2.2.H9 partially protected against the challenge with VACV, while only one mouse survived in the scFv-Fc-1.2.2.H9 group.
Article
Virology
Joe Holley, Rebecca P. Sumner, Sian Lant, Paolo Ribeca, David Ulaeto, Carlos Maluquer de Motes
Summary: The study shows that vaccinia virus regulates the production and maturation of different types of virions through the expression of the viral protein A26. A26 ensures sufficient viral dissemination and provides virions with enhanced environmental stability. This virus-regulated mechanism represents a novel level of complexity in mammalian viruses with significant impacts on evolution, adaptation, and transmission.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Zixi Yin, Ji-Li Chen, Yongxu Lu, Beibei Wang, Leila Godfrey, Alexander J. Mentzer, Xuan Yao, Guihai Liu, Dannielle Wellington, Yiqi Zhao, Peter A. C. Wing, Wanwisa Dejnirattisa, Piyada Supasa, Chang Liu, Philip Hublitz, Ryan Beveridge, Craig Waugh, Sally-Ann Clark, Kevin Clark, Paul Sopp, Timothy Rostron, Juthathip Mongkolsapaya, Gavin R. Screaton, Graham Ogg, Katie Ewer, Andrew J. Pollard, Sarah Gilbert, Julian C. Knight, Teresa Lambe, Geoffrey L. Smith, Tao Dong, Yanchun Peng
Summary: This study evaluates T cell responses in recovered COVID-19 patients and vaccinated donors using recombinant vaccinia virus (rVACV) expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of ACE-2-transduced B cell lines. The results show that rVACV expression of SARS-CoV-2 antigen can be used to assess T cell responses to naturally processed spike antigens. The rVACV system can also be used to evaluate cross-reactivity of memory T cells to variants of concern and identify epitope escape mutants.
Article
Multidisciplinary Sciences
Balazs Kakuk, Akos Dormo, Zsolt Csabai, Gabor Kemenesi, Jiri Holoubek, Daniel Ruzek, Istvan Prazsak, Virag eva Dani, Bela Denes, Gabor Torma, Ferenc Jakab, Gabor E. Toth, Fanni V. Foldes, Brigitta Zana, Zsofia Lanszki, Akos Harangozo, Adam Fulop, Gabor Gulyas, Mate Mizik, Andras Attila Kiss, Dora Tombacz, Zsolt Boldogkoi
Summary: The recent Monkeypox outbreak highlighted the importance of studying the basic biology of orthopoxviruses. However, there has been no investigation into the transcriptome of the causative agent using either short-read or long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap and allows for the comprehensive characterization of the monkeypox virus transcriptomic architecture and the potential annotation of novel host transcripts. Additionally, this study will provide insights into gene expression changes in both the virus and host cells during infection, leading to a deeper understanding of the alterations caused by viral infection at the transcriptome level.
Review
Biochemistry & Molecular Biology
Ryan C. Bruneau, Loubna Tazi, Stefan Rothenburg
Summary: In this article, we review the history of the evolution of cowpox viruses (CPXVs) and their broad host range, lethal outbreaks, and increasing human cases in Eurasian countries. Genetic analyses have revealed multiple Orthopoxvirus species categorized under CPXV designation. We discuss modern outbreaks in zoos, domesticated animals, and humans, including the pathogenesis, geographic range, and molecular level interactions. The potential threat of these viruses and the future of CPXV research are also discussed to provide a comprehensive review of CPXVs.
Review
Immunology
Rakesh Kulkarni, Erik A. C. Wiemer, Wen Chang
Summary: Lipid rafts are important components of cell membranes and play crucial roles in cellular processes and signaling. Both pathogens and hosts utilize lipid rafts to survive and initiate antimicrobial responses. This review focuses on the enrichment of major vault protein in lipid rafts during vaccinia virus infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Aline R. V. Souza, Annika Brinkmann, Jose Esparza, Andreas Nitsche, Clarissa R. Damaso, Rino Rappuoli
Summary: This study investigates the gene content and genomic structure of historical smallpox vaccines used in the late 19th and early 20th centuries. The findings reveal differences between these vaccines, contemporary vaccinia viruses, and horsepox viruses. The study suggests the coexistence of different horsepox-based vaccines and potentially unsampled progenitors of modern vaccinia virus. This research contributes to our understanding of the origins of contemporary vaccinia viruses and the evolution of smallpox vaccines.
Article
Virology
Masmudur M. Rahman, Grant McFadden
Summary: Poxviruses are a diverse family of DNA viruses that can cause diseases in selective hosts. Some poxviruses can cross host species and pose a genuine threat to human and animal health. These viruses are able to adapt to new hosts and evade the host's immune response by expressing self-defense proteins. Different cytokines play a vital role in controlling the host tropism and adaptation of poxviruses.
CURRENT OPINION IN VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Shyambabu Chaurasiya, Yuman Fong, Susanne G. Warner
Summary: Oncolytic viruses are a new class of therapeutics that have shown promise in clinical trials, although their anti-tumor efficacy as monotherapy is modest. Most oncolytic viruses are well tolerated, even in immune-suppressed patients, at the currently defined highest feasible doses. Enhancing the potency of oncolytic viruses is necessary for more viruses to be used effectively in clinical settings, thus they are being studied in combination with other therapies for higher therapeutic benefits.
Article
Immunology
Marsha S. Russell, Sathya N. Thulasi Raman, Caroline Gravel, Wanyue Zhang, Annabelle Pfeifle, Wangxue Chen, Gary Van Domselaar, David Safronetz, Michael Johnston, Simon Sauve, Lisheng Wang, Michael Rosu-Myles, Jingxin Cao, Xuguang Li
Summary: A novel recombinant RSV vaccine candidate based on a deletion mutant vaccinia virus platform showed high safety and efficacy in experiments, suggesting its potential for developing new vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Entao Li, Xiaoping Guo, Dongxiang Hong, Qizan Gong, Wenyu Xie, Tingting Li, Jian Wang, Xia Chuai, Sandra Chiu
Summary: This study found that individuals vaccinated with smallpox vaccine in China still maintain a certain level of humoral immunity, particularly targeting the A35R and B6R antigens. Additionally, there is a linear correlation between VTT-specific IgG antibodies and A35R-specific IgG, B6R-specific IgG, and A29L-specific IgG antibodies.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Microbiology
Kevin Chiem, Maria M. Lorenzo, Javier Rangel-Moreno, Maria De la Luz Garcia-Hernandez, Jun-Gyu Park, Aitor Nogales, Rafael Blasco, Luis Martinez-Sobrido
Summary: Recombinant vaccinia viruses expressing two reporter genes, fluorescent (Scarlet or GFP) and luciferase (Nluc), provide a valuable tool to study viral infection dynamics in vitro and in vivo, including host-pathogen interactions and tropism of viral infections. This dual reporter-expressing rVV enhances the ability to track viral infections in cultured cells and animal models.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Nicolas Berthet, Stephane Descorps-Declere, Camille Besombes, Manon Curaudeau, Andriniaina Andy Nkili Meyong, Benjamin Selekon, Ingrid Labouba, Ella Cyrielle Gonofio, Rita Sem Ouilibona, Huguette Dorine Simo Tchetgna, Maxence Feher, Arnaud Fontanet, Mirdad Kazanji, Jean-Claude Manuguerra, Alexandre Hassanin, Antoine Gessain, Emmanuel Nakoune
Summary: The study aimed to sequence the full genome of 10 MPXV isolates collected during CAR epidemics from 2001 to 2018 to determine their phylogenetic relationships among previously described MPXV lineages in Central Africa. The results showed that the 10 CAR isolates belong to lineages closely related to those found in the DRC, suggesting transmissions from wild animals living in the rainforest. This highlights the importance of understanding the socio-economic situation, armed conflicts, and ecological disturbances in CAR, which may increase the risk of zoonotic spillover.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Immunology
Thomas T. Murooka, Radwa R. Sharaf, Thorsten R. Mempel
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2015)
Article
Multidisciplinary Sciences
Xaver Sewald, Mark S. Ladinsky, Pradeep D. Uchil, Jagadish Beloor, Ruoxi Pi, Christin Herrmann, Nasim Motamedi, Thomas T. Murooka, Michael A. Brehm, Dale L. Greiner, Leonard D. Shultz, Thorsten R. Mempel, Pamela J. Bjorkman, Priti Kumar, Walther Mothes
Article
Virology
Menelaos Symeonides, Thomas T. Murooka, Lauren N. Bellfy, Nathan H. Roy, Thorsten R. Mempel, Markus Thali
Article
Multidisciplinary Sciences
Stanley Adoro, Juan R. Cubillos-Ruiz, Xi Chen, Maud Deruaz, Vladimir D. Vrbanac, Minkyung Song, Suna Park, Thomas T. Murooka, Timothy E. Dudek, Andrew D. Luster, Andrew M. Tager, Hendrik Streeck, Brittany Bowman, Bruce D. Walker, Douglas S. Kwon, Vanja Lazarevic, Laurie H. Glimcher
NATURE COMMUNICATIONS
(2015)
Article
Multidisciplinary Sciences
Alexandre P. Benechet, Manisha Menon, Daqi Xu, Tasleem Samji, Leigh Maher, Thomas T. Murooka, Thorsten R. Mempel, Brian S. Sheridan, Francois M. Lemoine, Kamal M. Khanna
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Oncology
Ahmed Y. Ali, Xun Wu, Nour Eissa, Sen Hou, Jean-Eric Ghia, Thomas T. Murooka, Versha Banerji, James B. Johnston, Francis Lin, Spencer B. Gibson, Aaron J. Marshall
Article
Multidisciplinary Sciences
Diego Ulisse Pizzagalli, Yagmur Farsakoglu, Miguel Palomino-Segura, Elisa Palladino, Jordi Sintes, Francesco Marangoni, Thorsten R. Mempel, Wan Hon Koh, Thomas T. Murooka, Flavian Thelen, Jens V. Stein, Giuseppe Pozzi, Marcus Thelen, Rolf Krause, Santiago Fernandez Gonzalez
Article
Immunology
Francesco Marangoni, Thomas T. Murooka, Teresa Manzo, Edward Y. Kim, Esteban Carrizosa, Natalie M. Elpek, Thorsten R. Mempel
Article
Immunology
Thomas T. Murooka, Thorsten R. Mempel
JOURNAL OF INFECTIOUS DISEASES
(2013)
Article
Immunology
Mathieu Angin, Siddhartha Sharma, Melanie King, Thomas T. Murooka, Musie Ghebremichael, Thorsten R. Mempel, Bruce D. Walker, Manoj K. Bhasin, Marylyn M. Addo
JOURNAL OF INFECTIOUS DISEASES
(2014)
Review
Microbiology
Oliver T. Fackler, Thomas T. Murooka, Andrea Imle, Thorsten R. Mempel
NATURE REVIEWS MICROBIOLOGY
(2014)
Article
Virology
Paul Lopez, Wan Hon Koh, Ryan Hnatiuk, Thomas T. Murooka
JOURNAL OF VIROLOGY
(2019)
Article
Multidisciplinary Sciences
Wan Hon Koh, Paul Lopez, Oluwaseun Ajibola, Roshan Parvarchian, Umar Mohammad, Ryan Hnatiuk, Jason Kindrachuk, Thomas T. Murooka
Article
Virology
Sarah Brendle, Jingwei J. Li, Nancy M. Cladel, Debra A. Shearer, Lynn R. Budgeon, Karla K. Balogh, Hannah Atkins, Marina Costa-Fujishima, Paul Lopez, Neil D. Christensen, John Doorbar, Thomas T. Murooka, Jiafen Hu
Summary: In this study using a mouse papillomavirus infection model, the impact of L1 and L2 on viral life cycle and tumor growth was investigated. The results showed that L1 is not essential for tumor growth induced by MmuPV1, and the L2 gene influences the expression and localization of the L1 protein in vivo.
Review
Microbiology
Nnamdi Ikeogu, Oluwaseun Ajibola, Romaniya Zayats, Thomas T. Murooka
Summary: This review discusses the role of memory T cells in HIV infection and how HIV hijacks normal immune processes to establish long-term infection.