Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Medicinal
Han Yan, Jie Wang, Jiayi Yang, Zhongqiu Xu, Chunxia Li, Cui Hao, Shixin Wang, Wei Wang
Summary: This study explored the anti-HSV-2 activity of a marine sulfated polysaccharide known as PSSD. It was found that PSSD demonstrated significant anti-HSV-2 activity with low cytotoxicity in vitro. PSSD was observed to directly interact with virus particles to prevent virus adsorption and inhibit virus-induced membrane fusion. In vivo experiments showed that PSSD effectively attenuated genital herpes symptoms, reduced virus shedding, and outperformed acyclovir. These findings highlight the potential of PSSD as a novel anti-genital herpes agent.
Article
Immunology
Mette Ratzer Freytag, Sofie Eg Jorgensen, Michelle Molgaard Thomsen, Ali Al-Mousawi, Alon Schneider Hait, David Olagnier, Jakob T. Bay, Marie Helleberg, Trine H. Mogensen
Summary: This study describes a 19-year-old woman with systemic HSV-1 infection and HLH, as well as a fatal course of neonatal herpesvirus infection postpartum. Investigation showed impaired antiviral responses in the mother's cells, potentially caused by variants in CASP8 or other noncoding regions of the genome.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Jie Wang, Kun-Te Shang, Qiong-Hong Ma, Zhao-Ying Dong, Yi-Hong Chen, Yu-Feng Yao
Summary: This study aimed to determine whether HSV-1 can be transmitted through TNTs and to investigate the effect of inhibiting the Arp2/3 complex on the intercellular transmission of HSV-1. The results showed that HSV-1 can be transmitted through TNTs and that inhibiting the Arp2/3 complex reduces the number of TNTs and the spread of HSV-1. This finding provides new insights into the transmission mode of HSV-1 and suggests a potential new antiviral target.
Article
Virology
Edward Trybala, Nadia Peerboom, Beata Adamiak, Malgorzata Krzyzowska, Jan-Ake Liljeqvist, Marta Bally, Tomas Bergstrom
Summary: The study shows that the HSV-2 mutant deficient in mgG expression has a defect in releasing progeny virions from infected cells. The mgG of HSV-2 plays a critical role in balancing virus interactions with GAG chains, preventing trapping of progeny virions on infected cell surfaces. This balance is necessary to ensure proper release of virions.
Article
Biochemistry & Molecular Biology
Pu Huang, Xu Wang, Mengyue Lei, Ying Ma, Hongli Chen, Jing Sun, Yunzhang Hu, Jiandong Shi
Summary: This study investigates the metabolic interaction between host cells and HSV-1. By analyzing the metabolic profiles in HSV-1-infected lung fibroblasts, the researchers found that HSV-1 induces metabolic reprogramming in host cells to promote or resist viral replication. They confirmed that the addition of the tryptophan metabolite kynurenine promotes viral replication, while the addition of 25-Hydroxycholesterol inhibits replication. Additionally, HSV-1 replication was enhanced in choline metabolic rate-limiting enzyme-deficient mouse macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Fujun Hou, Zeyu Sun, Yue Deng, Siyu Chen, Xiyuan Yang, Feiyang Ji, Menghao Zhou, Keyi Ren, Dongli Pan
Summary: This study investigates the targets of ICP0 in HSV-1 infection and its roles in neuronal cells. Mass spectrometry analysis identified various proteins interacting with ICP0 in Neuro-2a and 293T cells. Further experiments confirmed that SNX9 and OTUD4 restrict the replication of ICP0-null virus in neuronal cells, and OTUD4 enhances the expression of type I interferon during infection with ICP0-null virus.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ines Ripa, Sabina Andreu, Jose Antonio Lopez-Guerrero, Raquel Bello-Morales
Summary: The article discusses the role of the HSV-1 viral protein ICP34.5 in controlling cellular antiviral responses and debates its controversial functions such as autophagy inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Qing Fan, Richard Longnecker, Sarah A. Connolly
Summary: The viral fusion protein glycoprotein B (gB) is essential for virus entry in all herpesviruses. By introducing structure-based mutations, researchers identified mutations that counteracted a slow-entry phenotype in the virus and found that these mutations were located in different regions of the gB protein.
JOURNAL OF VIROLOGY
(2021)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Neurosciences
Domenica Donatella Li Puma, Maria Elena Marcocci, Giacomo Lazzarino, Giovanna De Chiara, Barbara Tavazzi, Anna Teresa Palamara, Roberto Piacentini, Claudio Grassi
Summary: Astrocytes release extracellular ATP upon HSV-1 infection, activating P2 receptors on neurons and astrocytes through a GSK-3-dependent pathway, facilitating viral entry and replication. Inhibition of astrocyte metabolism or blockade of P2 receptors or GSK-3 activation can reduce HSV-1 infection in both neurons and astrocytes, highlighting the critical role of astrocytes in regulating neuronal response to microbial stimuli.
Article
Immunology
Malgorzata Krzyzowska, Anders Jarneborn, Karolina Thorn, Kristina Eriksson, Tao Jin
Summary: This study provides evidence that treatment with tofacitinib may increase the risk of disease aggravation and severe encephalitis in primary herpes simplex infection by impairing antiviral response induced by monocytes and microglia.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
David Bernstein, Derek A. Pullum, Rhonda D. Cardin, Fernando J. Bravo, David A. Dixon, Konstantin G. Kousoulas
Article
Veterinary Sciences
Natalie Fowlkes, Kelli Clemons, Paul J. F. Rider, Ramesh Subramanian, Nobuko Wakamatsu, Ingeborg Langohr, Konstantin G. Kousoulas
COMPARATIVE MEDICINE
(2019)
Article
Multidisciplinary Sciences
David I. Bernstein, Rhonda D. Cardin, Derek A. Pullum, Fernando J. Bravo, Konstantin G. Kousoulas, David A. Dixon
Article
Virology
Muzammel Haque, K. G. Kousoulas
JOURNAL OF VIROLOGY
(2019)
Article
Cell Biology
Jean Christopher Chamcheu, Stephane Esnault, Vagar M. Adhami, Andrea L. Noll, Sergette Banang-Mbeumi, Tithi Roy, Sitanshu S. Singh, Shile Huang, Konstantin G. Kousoulas, Hasan Mukhtar
Article
Genetics & Heredity
Andrew C. Lewin, Lyndon M. Coghill, Gillian J. McLellan, Ellison Bentley, Konstantin G. Kousoulas
Article
Multidisciplinary Sciences
Shan K. Naidu, Rafiq Nabi, Nagarjuna R. Cheemarla, Brent A. Stanfield, Paul J. Rider, Nithya Jambunathan, Vladimir N. Chouljenko, Renee Carter, Fabio Del Piero, Ingeborg Langohr, Konstantin G. Kousoulas
Article
Virology
Farhana Musarrat, Vladimir Chouljenko, Achyut Dahal, Rafiq Nabi, Tamara Chouljenko, Seetharama D. Jois, Konstantin G. Kousoulas
JOURNAL OF MEDICAL VIROLOGY
(2020)
Article
Virology
Farhana Musarrat, Vladimir Chouljenko, Konstantin G. Kousoulas
Summary: Herpes simplex virus 1 utilizes cellular motor proteins and kinase pathways for intracellular virion transport, with Akt and kinase C inhibitors blocking virus entry and virion capsid transport. UL37 and Vp5 viral proteins are crucial for dynein-dependent transport of virion capsids, while Akt and kinase C do not directly phosphorylate or activate dynein during infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tithi Roy, Samuel T. Boateng, Sergette Banang-Mbeumi, Pankaj K. Singh, Pratik Basnet, Roxane-Cherille N. Chamcheu, Federico Ladu, Isabel Chauvin, Vladimir S. Spiegelman, Ronald A. Hill, Konstantin G. Kousoulas, Bolni Marius Nagalo, Anthony L. Walker, Jean Fotie, Siva Murru, Mario Sechi, Jean Christopher Chamcheu
Summary: Novel flavonol-based derivatives targeting skin cancers were synthesized and characterized, showing significant inhibitory effects on human skin cancer cell lines. These compounds were found to induce apoptosis of cancer cells by regulating key molecular targets, presenting promising therapeutic potential for melanoma and non-melanoma skin cancers.
BIOORGANIC CHEMISTRY
(2021)
Article
Virology
Jared S. Rudd, Farhana Musarrat, Konstantin G. Kousoulas
Summary: BoHV-1 infects bovine-derived neuronal cells and can be efficiently studied using FBBC-1 cells. The study provides insights into virus entry and spread mechanisms in a neuronal cell culture system.
Review
Virology
Ifeanyi Kingsley Uche, Konstantin G. Kousoulas, Paul J. F. Rider
Summary: The development of cancer disrupts anti-tumor immunity needed for surveillance and elimination of tumor cells, with immunotherapy aiming to restore or establish these responses. However, a significant proportion of patients do not respond equally to immunotherapies, partially due to the immunosuppressive tumor microenvironment. Current research focuses on mechanisms of immunosuppression in the TME, with the goal of promoting anti-tumor immune responses.
Article
Multidisciplinary Sciences
Tithi Roy, Samuel T. Boateng, Sergette Banang-Mbeumi, Pankaj K. Singh, Pratik Basnet, Roxane-Cherille N. Chamcheu, Federico Ladu, Isabel Chauvin, Vladimir S. Spiegelman, Ronald A. Hill, Konstantin G. Kousoulas, Bolni Marius Nagalo, Anthony L. Walker, Jean Fotie, Siva Murru, Mario Sechi, Jean Christopher Chamcheu
Summary: This study identifies a panel of novel fisetin analogs that may be developed as potential therapeutic agents for melanoma and non-melanoma skin cancers, providing in-depth biological characterization data to support further research and experimental screening.
Article
Virology
Ifeanyi Kingsley Uche, Natalie Fowlkes, Luan Vu, Tatiane Watanabe, Mariano Carossino, Rafiq Nabi, Fabio del Piero, Jared S. Rudd, Konstantin G. Kousoulas, Paul J. F. Rider
Summary: Oncolytic virotherapy (OVT) uses viral infection to release tumor antigens, promoting antitumor immune responses. VC2, a modified herpes simplex virus, shows clinical potential in a mouse melanoma model and may be an effective treatment for human and animal cancers.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Paul J. F. Rider, Ifeanyi K. Uche, Larissa Sweeny, Konstantin G. Kousoulas
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2019)