Article
Biochemical Research Methods
Latief A. Wani, Priyanka Jawa, Jawaid A. Khan
Summary: Apple mosaic virus (ApMV) and Prunus necrotic ringspot virus (PNRSV), belonging to genus Ilarvirus, cause significant losses to rose and other plants of the family Rosaceae. In this study, two primer sets were designed for the development of a colorimetric Reverse Transcriptase - Loop Mediated Isothermal Amplification (RT-LAMP) assay, which showed virus-specificity and high sensitivity for early detection of ApMV and PNRSV.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Biochemical Research Methods
Kean Hean Ooi, Mengying Mandy Liu, Jia Rong Moo, Pattaraporn Nimsamer, Sunchai Payungporn, Pornchai Kaewsapsak, Meng How Tan
Summary: The COVID-19 pandemic has led to a high demand for frequent testing, and RT-LAMP has emerged as a promising diagnostic platform for rapid detection of SARS-CoV-2. However, spurious amplicons can affect the accuracy of RT-LAMP assays. This study developed a sensitive RT-LAMP assay that utilizes a sequence-specific probe to prevent false amplification. Clinical validation showed that this diagnostic test has high specificity, positive predictive value, and sensitivity, making it an inexpensive method for point-of-need diagnosis of COVID-19 and other infectious diseases.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Zhengkang Li, Yuwei Di, Xiaoyan Song, Yanqi Wu, Yingye Feng, Xinqiang Zhang, Caiping Gong, Guanghua Li
Summary: In this study, a quantitative detection method for NoV GII was established, and the RT-LAMP detection system was found to be a viable alternative to RT-PCR for qualitative detection of NoV. Compared to RT-PCR, RT-LAMP offers shorter detection time and lower technical requirements.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Virology
Marco Clementino, Karene F. Cavalcante, Vania A. F. Viana, Dayara de Oliveira Silva, Caroline R. Damasceno, Jessica Fernandes de Souza, Rafhaella N. D. G. Gondim, Daniel M. de Melo Jorge, Lyvia M. V. C. Magalhaes, Erico A. G. de Arruda, Roberto da J. P. Neto, Melissa S. Medeiros, Arnnenio A. dos Santos, Pedro J. C. Magalhaes, Liana P. Mello, Eurico Arruda, Aldo A. M. Lima, Alexandre Havt
Summary: This study adopted the RT-LAMP technique to detect SARS-Cov-2 in patient samples and compared it with the RT-qPCR technique. The results showed that the RT-LAMP technique using primers N5 and Orf9 had a sensitivity of 96% and specificity of 85%, with accurate diagnosis obtained only in nasopharyngeal swabs processed via an extraction kit.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Waqas Waheed, Sueda Saylan, Taimur Hassan, Hussain Kannout, Habiba Alsafar, Anas Alazzam
Summary: This paper presents a deep learning-driven portable device for rapid detection of COVID-19 using Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). The device can perform 16 simultaneous RT-LAMP reactions, and the experimental protocol eliminates the need for separate RNA extraction equipment and sample evaporation. The entire process takes only 45 minutes, and the device is coupled with a deep learning system to automatically analyze the results and screen COVID-19 subjects.
SCIENTIFIC REPORTS
(2022)
Review
Biotechnology & Applied Microbiology
Praveen Rai, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit, Indrani Karunasagar, Iddya Karunasagar
Summary: COVID-19 is a disease caused by SARS-CoV-2 that has spread globally, requiring collaborative efforts for accurate diagnosis and prevention. While RT-qPCR detection is considered the gold standard for early virus detection, challenges such as technical complexity and limited applicability have led to the development of portable POC tests.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Maria Dolores Feltner, Romina Bonaventura, Jorge Basiletti, Martin Avaro, Estefania Benedetti, Ana Campos, Maria Elena Dattero, Mara Russo, Sara Vladmirsky, Viviana Molina, Lucia Irazu, Marcelo A. Rodriguez, Andrea Pontoriero, Daniel M. Cisterna, Elsa G. Baumeister
Summary: The study evaluated the analytical and clinical performance of SARS-CoV-2 molecular detection kits in Argentina. RT-qPCR kits showed high kappa values and 100% specificity with varying sensitivity based on the gene analyzed. This information can help in selecting appropriate diagnostic tests for SARS-CoV-2 in Argentina and the region.
Article
Biology
Robert Warneford-Thomson, Parisha P. Shah, Patrick Lundgren, Jonathan Lerner, Jason Morgan, Antonio Davila, Benjamin S. Abella, Kenneth Zaret, Jonathan Schug, Rajan Jain, Christoph A. Thaiss, Roberto Bonasio
Summary: The COVID-19 pandemic has created an urgent need for rapid, effective, and low-cost SARS-CoV-2 diagnostic testing. COV-ID is an approach that combines RT-LAMP with deep sequencing to detect SARS-CoV-2 in unprocessed human saliva. The method is highly flexible and scalable, allowing for simultaneous detection of multiple amplicons and multiple pathogens.
Article
Chemistry, Analytical
Wansadaj Jaroenram, Itthi Chatnuntawech, Jantana Kampeera, Sukanya Pengpanich, Pornsawan Leaungwutiwong, Benyatip Tondee, Sarawut Sirithammajak, Rapheephat Suvannakad, Pakapreud Khumwan, Sirintip Dangtip, Narong Arunrut, Sirasate Bantuchai, Wang Nguitragool, Suchawit Wongwaroran, Paisan Khanchaitit, Jetsumon Sattabongkot, Surat Teerapittayanon, Wansika Kiatpathomchai
Summary: Colorimetric loop-mediated DNA isothermal amplification-based assays using pH-dependent indicators have been developed for COVID-19 diagnosis. The dual colorimetric RT-LAMP assay with artificial intelligence (AI) operated tool enables precise and rapid result analysis in large scale testing. The method offers a simpler, faster, and cheaper approach for COVID-19 testing and other emerging pathogens.
Article
Virology
Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo
Summary: Early detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) is crucial for appropriate treatment and prevention of further transmission. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an effective method for detecting the SFTSV genome, with the simplified RT-LAMP showing high sensitivity and specificity in detecting SFTSV in patient sera during the early phase of the disease.
Article
Chemistry, Multidisciplinary
Chao Ji, Shuxia Xue, Min Yu, Jinyu Liu, Qin Zhang, Feng Zuo, Qiuyue Zheng, Liangjuan Zhao, Hongwei Zhang, Jijuan Cao, Ke Wang, Wei Liu, Wenjie Zheng
Summary: The study introduced a rapid and simple method for detecting the SARS-CoV-2 virus, which can be easily detected by naked eye through color change, demonstrating high specificity and accuracy.
Article
Fisheries
Meijuan Shi, Yanyan Cao, Xiucai Hu, Fuli Luo, Aijun Lv
Summary: This study presents the first report of RT-qPCR and LAMP methods for the detection of R. aquatilis, providing a reliable means for its rapid detection and diagnosis in fish.
Article
Nanoscience & Nanotechnology
Kaizhen Wen, Yanyu Cai, Yuhong Guan
Summary: This research aimed to design a dual LAMP fluorescence detection system for HuNoVs, which can accurately detect the ORF1-ORF2 junction region, the most conserved area in HuNoVs genomes I and II. A rapid integrated detection platform for pediatric norovirus emergencies was built using a commercially available system based on magnetic bead-based nucleic acid extraction and LAMP fluorescence detection. The method showed cross-reactivity with four other viruses and demonstrated consistent results with conventional laboratory extracts for 28 clinical samples.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhongjie Fei, Rongbin Wei, Chu Cheng, Pengfeng Xiao
Summary: The study developed an improved and sensitive approach to SARS-CoV-2 detection using BART and RT-LAMP protocols, which was also compatible with a digital LAMP system. By improving RNA availability in samples and utilizing a simulated digital RT-LAMP approach, real-time detection with a lower limit of detection was achieved, along with enhancements in the overall dynamic range of the assay system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yue Tan, Hai-Rong Wei, Jia-Wei Wang, Xiao-Juan Zong, Dong-Zi Zhu, Qing-Zhong Liu
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2015)
Article
Virology
Jiawei Wang, Dongzi Zhu, Yue Tan, Xiaojuan Zong, Hairong Wei, Rosemarie W. Hammond, Qingzhong Liu
ARCHIVES OF VIROLOGY
(2016)
Article
Genetics & Heredity
Xiao-Cui Luo, Mei-Hong Sun, Rui-Rui Xu, Huai-Rui Shu, Jia-Wei Wang, Shi-Zhong Zhang
JOURNAL OF GENETICS
(2014)
Article
Horticulture
Chao Gu, Xiao-Juan Zong, Jia-Wei Wang, Hai-Rong Wei, Qing-Zhong Liu
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2013)
Article
Multidisciplinary Sciences
Jiawei Wang, Weizhen Liu, Dongzi Zhu, Xiang Zhou, Po Hong, Hongjun Zhao, Yue Tan, Xin Chen, Xiaojuan Zong, Li Xu, Lisi Zhang, Hairong Wei, Qingzhong Liu
Article
Plant Sciences
Jiawei Wang, Weizhen Liu, Dongzi Zhu, Po Hong, Shizhong Zhang, Shijun Xiao, Yue Tan, Xin Chen, Li Xu, Xiaojuan Zong, Lisi Zhang, Hairong Wei, Xiaohui Yuan, Qingzhong Liu
HORTICULTURE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Li Xu, Xiaojuan Zong, Jiawei Wang, Hairong Wei, Xin Chen, Qingzhong Liu
Article
Plant Sciences
Yanping Zhang, Jing Peng, Xiaohui Yuan, Lisi Zhang, Dongzi Zhu, Po Hong, Jiawei Wang, Qingzhong Liu, Weizhen Liu
Summary: A novel cultivar identification system called MFCIS was proposed, which achieved high accuracy by combining persistent homology and CNN to extract multiple leaf morphological features. The system was validated on sweet cherry and soybean datasets, demonstrating an effective method for plant breeders to identify plant cultivars efficiently.
HORTICULTURE RESEARCH
(2021)
Article
Agronomy
W. Wei, E. Perez-Lopez, R. E. Davis, L. Bermudez-Diaz, C. Granda-Wong, J. Wang, Y. Zhao
Article
Chemistry, Analytical
Wei Hairong, Yi Xibin, Tan Yue, Zong Xiaojuan, Wang Jiawei, Xu Li, Liu Qingzhong
CHINESE JOURNAL OF CHROMATOGRAPHY
(2015)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
