Article
Chemistry, Applied
Jinchun Li, Jiapeng Li, Yixuan Wei, Suigen Xu, Sueyue Xiong, Dan Li, Shouwei Wang, Anqi Liang
Summary: Meat adulteration is a significant issue in the meat supply chain, and it can seriously harm consumers' economic and health interests. To address the problem of mutton adulteration and reduce testing costs, a triplex real-time PCR method with melting curve analysis was developed to identify three meat categories simultaneously.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Multidisciplinary Sciences
Qiuying Huang, Dongmei Chen, Chen Du, Qiaoqiao Liu, Su Lin, Lanlan Liang, Ye Xu, Yiqun Liao, Qingge Li
Summary: The article introduces a method called MeltArray, which allows for the detection of multiple targets in a single real-time PCR reaction. This method utilizes the enzyme activity of Taq DNA polymerase to generate fluorescent hybrids with different melting temperatures, enabling the detection of multiple targets. MeltArray has been validated with clinical samples and demonstrates the advantages of multiplicity, versatility, simplicity, and accessibility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Wen Yu, Juan Yao, Zhang Zhang
Summary: Genotyping of folate metabolism genes is crucial for disease diagnosis and prevention. In this study, a multiplex asymmetric PCR-HRM strategy was developed for identifying genotypes of folate metabolism genes. This approach offers advantages such as multiplex detection, high accuracy, and resistance to aerosol pollution, making it a promising platform for clinical diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Jinchun Li, Jiapeng Li, Ruixi Liu, Yixuan Wei, Shouwei Wang
Summary: The study developed a hexaplex real-time PCR assay using melting curve analysis to identify eleven meat species simultaneously, showing good specificity of primers and detection limits of 0.01-0.1 ng DNA and 0.1-1% meat mixture. The method can accurately identify different meat species, indicating great application potential.
Article
Chemistry, Analytical
Wen Yu, Juan Yao, Zhang Zhang
Summary: In this study, a multiplex asymmetric PCR-HRM strategy was developed for identifying genotypes of folate metabolism genes in a single tube. The assay demonstrated high accuracy and resistance to aerosol pollution. This approach could potentially establish a fast and cost-effective platform for folate metabolism gene genotyping and other SNP genotyping in clinical diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Ane del Rio-Lavin, Elisa Jimenez, Miguel Angel Pardo
Summary: The study developed a real-time PCR assay for the identification of Mytilus spp. mussels in the European market, showing 100% specificity, sensitivity, and precision, and can be easily applied across different platforms without special software requirements.
Article
Agronomy
Leifeng Xu, Meng Song, Jun Ming
Summary: In this study, a rapid and sensitive multiplex TaqMan real-time PCR assay was developed to simultaneously detect and quantify five economically important viruses infecting lilies. The assay showed high specificity, sensitivity, and reproducibility and can be used for certification and quarantine programs.
Article
Virology
Yi Zheng, Youyun Zhao, Yefu Wang, Jun Rao
Summary: Fluorescent quantitative PCR assay is widely used for detecting viral DNA, but a new method has been developed to simultaneously detect HHV-6, 7, and 8. Through blood sample collection and PCR optimization, a triple fluorescent quantitative PCR detection system was constructed for the simultaneous detection of HHV-6, 7, and 8. The method showed good specificity and sensitivity in blood transfusion screening.
Article
Chemistry, Applied
Wenjie Xu, Mingyang Fu, Manhong Huang, Xiaowen Cui, Yi Li, Min Cao, Libin Wang, Xiaohui Xiong, Xiong Xiong
Summary: A novel method for rapid identification of S. solar and O. mykiss based on duplex PCR assay was developed successfully in this study. Specific primers design and melting curve analysis enabled accurate discrimination of the two fish species, offering a feasible solution for detecting adulteration in processed fish products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Microbiology
Yuan Chen, Shile Luo, Jianmei Tan, Luhua Zhang, Shengwu Qiu, Zhiyou Hao, Naidong Wang, Zhibang Deng, Aibing Wang, Qing Yang, Yi Yang, Changjian Wang, Yang Zhan
Summary: Many pathogens cause reproductive failure in sows, and various detection methods have been used for molecular diagnosis. In this study, a multiplex real-time PCR method was developed for the simultaneous detection of PCV2, PCV3, PPV, and PRV associated with porcine reproductive failure. The method showed high specificity, good repeatability, and accurate detection of these four pathogens. It has practical applications in diagnostics, surveillance, and epidemiology.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Zhixiang Xu, Yun Peng, Minghui Yang, Xiaohe Li, Jun Wang, Rongrong Zou, Jinhu Liang, Shisong Fang, Yingxia Liu, Yang Yang
Summary: Due to the prevalence and risk of coinfection of multiple arboviruses, a multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection, with high specificity and sensitivity.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Eiseul Kim, Dayoung Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: In this study, a multiplex real-time PCR method was developed to identify and differentiate three closely related species in the Lactobacillus acidophilus group. The method showed high specificity and sensitivity, with a detection limit of 10(1) CFU/ml. It can be used for monitoring these species in probiotic and dairy products.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Fan Yang, Sijing Yan, Linwei Zhu, Hangping Yao, Dalu Dong, Danna Wu, Nanping Wu, Chunsheng Ye, Haibo Wu
Summary: Avian influenza viruses (AIVs), including highly pathogenic and low pathogenic subtypes, have caused epidemics in birds and posed a health challenge to humans. The low pathogenic subtypes, such as H4, H6, and H10, have covertly spread in domestic poultry and sporadically infected humans, indicating a potential pandemic risk. Therefore, there is an urgent need for a rapid and sensitive diagnostic method to simultaneously detect these AIV subtypes.
Article
Chemistry, Analytical
Kerou Zhang, Alessandro Pinto, Lauren Yuxuan Cheng, Ping Song, Peng Dai, Michael Wang, Luis Rodriguez, Cailin Weller, David Yu Zhang
Summary: In this study, we developed an occlusion system that functions as an efficient fluorescence indicator in HiFi qPCR reactions, improving the detection sensitivity for early cancer diagnosis and drug-resistance mutation identification.
ANALYTICAL CHEMISTRY
(2022)
Review
Virology
Tanja Opriessnig, Anbu K. Karuppannan, Alessandra M. M. G. Castro, Chao-Ting Xiao
Editorial Material
Medicine, Research & Experimental
Tanja Opriessnig, Yao-Wei Huang
XENOTRANSPLANTATION
(2020)
Article
Virology
Nguyen Thi Dieu Thuy, Nguyen Tran Trung, Tran Quoc Dung, Do Vo Anh Khoa, Dinh Thi Ngoc Thuy, Tanja Opriessnig
Summary: The study investigated the prevalence of PPV1-4 in pigs from slaughterhouses in Vietnam. PPV1 and PPV2 were found in 11 provinces, while PPV4 was detected in only three provinces. Co-circulation of PPV1, PPV2, and PPV3 was frequently observed, with predominance of PPV1/PPV2 coinfection at 20.8%.
ARCHIVES OF VIROLOGY
(2021)
Editorial Material
Medicine, Research & Experimental
Tanja Opriessnig, Yao-Wei Huang
XENOTRANSPLANTATION
(2021)
Review
Veterinary Sciences
Tanja Opriessnig, Ashley A. Mattei, Anbu K. Karuppannan, Patrick G. Halbur
Summary: The article discusses the history, types, and importance of variolation and pig vaccines in pig production, as well as the continuous innovation and application of vaccine strategies and tools in pigs with the advancement of genomic sequencing and molecular engineering technologies.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Biochemical Research Methods
Pei Nan, Dan Wen, Tanja Opriessnig, Qiuya Zhang, Xiaoya Yu, Yonghou Jiang
Summary: A novel UP-M-PCR assay was developed for simultaneous detection and differentiation of viral pathogens associated with diarrhea in pigs, showing rapid, highly sensitive, and specific detection capabilities, even in cases of mixed infections in pigs.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Microbiology
Matthias Eddicks, Roland Maurer, Pauline Deffner, Lina Eddicks, Wolfgang Sipos, Sven Reese, Vojislav Cvjetkovic, Roman Krejci, Tanja Opriessnig, Mathias Ritzmann, Robert Fux
Summary: PCV2 is highly prevalent in suckling piglets, while the prevalence of PCV3 is low. Vaccination of sows against PCV2 can protect piglets from PCV2 infection, and vaccination of sows against PRRSV can protect piglets from PCV3 infection. This indicates the importance of factors affecting the infection of PCV2 and PCV3 in pig populations.
Article
Multidisciplinary Sciences
Whitney E. Harrington, Winnie Yeung, Ingrid A. Beck, Fred D. Mast, John Houck, Sheila Styrchak, Leslie R. Miller, Song Li, Micaela Haglund, Yonghou Jiang, Blair Armistead, Jackson Wallner, Tina Nguyen, Daisy Ko, Samantha Hardy, Alyssa Oldroyd, Ana Gervassi, John D. Aitchison, Lisa M. Frenkel
Summary: This study found that weekly rtPCR surveillance did not efficiently detect asymptomatic infections in low-risk populations, while vaccination reduced the incidence of infection.
Review
Medicine, Research & Experimental
Tanja Opriessnig, Yao-Wei Huang
Summary: This review article summarizes the latest research findings on whether pigs can be infected with SARS-CoV-2. It suggests that pigs are currently considered a low-risk species, posing no immediate risk to the human population or xenotransplantation recipients. However, monitoring the ever-changing variants of the virus is crucial.
XENOTRANSPLANTATION
(2022)
Article
Microbiology
Ingrid A. Beck, Sheila Styrchak, Leslie Miller, Fred Mast, Vladimir Vigdorovich, Winnie Yeung, Daisy Ko, Alyssa Oldroyd, Samantha Hardy, Song Li, John Houck, Yonghou Jiang, Nicholas Dambrauskas, Catherine Darcey, Andrew Raappana, William Selman, D. Noah Sather, John D. Aitchison, Whitney E. Harrington, Lisa M. Frenkel
Summary: This study found that nasal secretions from biomedical personnel contained SARS-CoV-2 DNA, even though these individuals did not show any symptoms. It suggests that they may have been infected with SARS-CoV-2 circulating in the community or with SARS-CoV-2 DNA from a plasmid vector. The presence of nonviral plasmid vectors can interfere with molecular diagnostic tests.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Qiuya Zhang, Qin Liu, Tanja Opriessnig, Dan Wen, Keda Gu, Yonghou Jiang
Summary: Porcine astrovirus (PAstV) is associated with diarrhea in piglets, but more knowledge is needed. We developed a multiplex PCR assay to detect and differentiate the 5 genotypes of PAstV. Our assay amplified all 5 DNA fragments from single or mixed viral genomes without cross-reactions. The multiplex PCR assay provides a simple, sensitive, and specific detection tool for PAstV.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Microbiology
Gaurav Rawal, Wannarat Yim-im, Ethan Aljets, Patrick G. Halbur, Jianqiang Zhang, Tanja Opriessnig
Summary: Porcine respiratory coronavirus (PRCV) is a mutant strain distinct from transmissible gastroenteritis virus (TGEV). Little is known about recent PRCV isolates, and this study aimed to obtain a contemporary PRCV isolate from US pigs for genetic characterization.
Article
Microbiology
Adrian Muwonge, Anbu K. Karuppannan, Tanja Opriessnig
Summary: This study demonstrates that probiotic supplementation can increase ileum microbiota diversity, mitigating the impact of pathogenic Lawsonia intracellularis challenge. The research shows that an increase in ileum microbiota diversity is associated with a reduction in shedding.
Article
Pathology
Tanja Opriessnig, Chao-Ting Xiao, Patrick G. Halbur
JOURNAL OF COMPARATIVE PATHOLOGY
(2020)
Article
Immunology
Harini Sooryanarain, Connie L. Heffron, Dolores E. Hill, Jorrell Fredericks, Benjamin M. Rosenthal, Stephen R. Werre, Tanja Opriessnig, Xiang-Jin Meng
EMERGING INFECTIOUS DISEASES
(2020)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)