Article
Ophthalmology
Tarjani Dave, Savitri Sharma, Vemu Lakshmi, Ashok Rangaiahgari, Somasheila Murthy, Mohammad J. Ali, Vivek P. Dave, Rajeev R. Pappuru
Summary: This study reports three cases of infectious panophthalmitis post-dengue fever with ex vivo confirmation of dengue virus RNA in eye tissues. The presence of dengue virus in the eviscerated specimens suggests its involvement in the ocular infection. The patients recovered completely and maintained healthy eviscerated sockets.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Plant Sciences
Luis Rubio, Francisco J. J. Guinot-Moreno, Carmen Sanz-Lopez, Luis Galipienso
Summary: A new species of Sobemovirus, Xufa yellow dwarf virus, was discovered and identified as the causal agent of a disease affecting C. esculentus crops in Valencia, Spain. Fast and accurate methods for detecting and quantifying the virus were developed based on reverse transcription and DNA amplification. The virus was found to have a high incidence in both symptomatic and asymptomatic plants, with higher accumulation in symptomatic plants.
Article
Nanoscience & Nanotechnology
Zhengkang Li, Yuwei Di, Xiaoyan Song, Yanqi Wu, Yingye Feng, Xinqiang Zhang, Caiping Gong, Guanghua Li
Summary: In this study, a quantitative detection method for NoV GII was established, and the RT-LAMP detection system was found to be a viable alternative to RT-PCR for qualitative detection of NoV. Compared to RT-PCR, RT-LAMP offers shorter detection time and lower technical requirements.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Kean Hean Ooi, Mengying Mandy Liu, Jia Rong Moo, Pattaraporn Nimsamer, Sunchai Payungporn, Pornchai Kaewsapsak, Meng How Tan
Summary: The COVID-19 pandemic has led to a high demand for frequent testing, and RT-LAMP has emerged as a promising diagnostic platform for rapid detection of SARS-CoV-2. However, spurious amplicons can affect the accuracy of RT-LAMP assays. This study developed a sensitive RT-LAMP assay that utilizes a sequence-specific probe to prevent false amplification. Clinical validation showed that this diagnostic test has high specificity, positive predictive value, and sensitivity, making it an inexpensive method for point-of-need diagnosis of COVID-19 and other infectious diseases.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Virology
Marco Clementino, Karene F. Cavalcante, Vania A. F. Viana, Dayara de Oliveira Silva, Caroline R. Damasceno, Jessica Fernandes de Souza, Rafhaella N. D. G. Gondim, Daniel M. de Melo Jorge, Lyvia M. V. C. Magalhaes, Erico A. G. de Arruda, Roberto da J. P. Neto, Melissa S. Medeiros, Arnnenio A. dos Santos, Pedro J. C. Magalhaes, Liana P. Mello, Eurico Arruda, Aldo A. M. Lima, Alexandre Havt
Summary: This study adopted the RT-LAMP technique to detect SARS-Cov-2 in patient samples and compared it with the RT-qPCR technique. The results showed that the RT-LAMP technique using primers N5 and Orf9 had a sensitivity of 96% and specificity of 85%, with accurate diagnosis obtained only in nasopharyngeal swabs processed via an extraction kit.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Chemistry, Analytical
Junmin Li, Xuejiao Hu, Xiaoming Wang, Jianing Yang, Lei Zhang, Qianyun Deng, Xiqin Zhang, Zixia Wang, Tieying Hou, Shan Li
Summary: A novel one-tube rapid diagnostic approach based on formamide and colorimetric RT-LAMP was established for the detection of SARS-CoV-2 without the extraction of specific viral RNA. The technique showed high specificity and could visually detect the virus within 45 minutes.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Waqas Waheed, Sueda Saylan, Taimur Hassan, Hussain Kannout, Habiba Alsafar, Anas Alazzam
Summary: This paper presents a deep learning-driven portable device for rapid detection of COVID-19 using Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP). The device can perform 16 simultaneous RT-LAMP reactions, and the experimental protocol eliminates the need for separate RNA extraction equipment and sample evaporation. The entire process takes only 45 minutes, and the device is coupled with a deep learning system to automatically analyze the results and screen COVID-19 subjects.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Galyah Alhamid, Huseyin Tombuloglu, Ebtesam Al-Suhaimi
Summary: The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. We developed colorimetric and fluorometric RT-LAMP assays using five LAMP primers instead of six to overcome misamplifications and verified their performance with the gold-standard RT-PCR technique. The E-ID1 primer set, including five primers, performed superbly on both colorimetric and fluorometric assays, with high sensitivity, specificity, and accuracy.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Masato Inaba, Yuki Higashimoto, Yoko Toyama, Tomoya Horiguchi, Masaya Hibino, Mitsunaga Iwata, Kazuyoshi Imaizumi, Yohei Doi
Summary: The study demonstrates that RT-LAMP has a diagnostic accuracy comparable to RT-qPCR in the early phase of COVID-19, until the 9th day after symptom onset. However, after the 10th day, the positivity rate of RT-LAMP significantly decreases, suggesting its limitations in diagnosing COVID-19 in later stages of the disease.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Wansadaj Jaroenram, Itthi Chatnuntawech, Jantana Kampeera, Sukanya Pengpanich, Pornsawan Leaungwutiwong, Benyatip Tondee, Sarawut Sirithammajak, Rapheephat Suvannakad, Pakapreud Khumwan, Sirintip Dangtip, Narong Arunrut, Sirasate Bantuchai, Wang Nguitragool, Suchawit Wongwaroran, Paisan Khanchaitit, Jetsumon Sattabongkot, Surat Teerapittayanon, Wansika Kiatpathomchai
Summary: Colorimetric loop-mediated DNA isothermal amplification-based assays using pH-dependent indicators have been developed for COVID-19 diagnosis. The dual colorimetric RT-LAMP assay with artificial intelligence (AI) operated tool enables precise and rapid result analysis in large scale testing. The method offers a simpler, faster, and cheaper approach for COVID-19 testing and other emerging pathogens.
Article
Genetics & Heredity
Maria Dolores Feltner, Romina Bonaventura, Jorge Basiletti, Martin Avaro, Estefania Benedetti, Ana Campos, Maria Elena Dattero, Mara Russo, Sara Vladmirsky, Viviana Molina, Lucia Irazu, Marcelo A. Rodriguez, Andrea Pontoriero, Daniel M. Cisterna, Elsa G. Baumeister
Summary: The study evaluated the analytical and clinical performance of SARS-CoV-2 molecular detection kits in Argentina. RT-qPCR kits showed high kappa values and 100% specificity with varying sensitivity based on the gene analyzed. This information can help in selecting appropriate diagnostic tests for SARS-CoV-2 in Argentina and the region.
Article
Medicine, General & Internal
Juan Garcia-Bernalt Diego, Pedro Fernandez-Soto, Marta Dominguez-Gil, Moncef Belhassen-Garcia, Juan Luis Munoz Bellido, Antonio Muro
Summary: The global SARS-CoV-2 pandemic has brought about a need for widespread testing for COVID-19, with RT-qPCR as the gold standard method, but facing challenges. A novel RT-LAMP method has been developed, showing promise for cost-effective, rapid, and stable detection of SARS-CoV-2.
Article
Biology
Robert Warneford-Thomson, Parisha P. Shah, Patrick Lundgren, Jonathan Lerner, Jason Morgan, Antonio Davila, Benjamin S. Abella, Kenneth Zaret, Jonathan Schug, Rajan Jain, Christoph A. Thaiss, Roberto Bonasio
Summary: The COVID-19 pandemic has created an urgent need for rapid, effective, and low-cost SARS-CoV-2 diagnostic testing. COV-ID is an approach that combines RT-LAMP with deep sequencing to detect SARS-CoV-2 in unprocessed human saliva. The method is highly flexible and scalable, allowing for simultaneous detection of multiple amplicons and multiple pathogens.
Article
Infectious Diseases
Thanyarat Promlek, Maytawan Thanunchai, Uraporn Phumisantiphong, Tonsan Hansirisathit, Chayanit Phuttanu, Sunisa Dongphooyao, Wipawee Thongsopa, Pornlada Nuchnoi
Summary: The clinical performance of the FastProof 30 min-TTR SARS-CoV-2 RT-LAMP method was validated in this study. The results showed that RT-LAMP had high sensitivity and specificity in detecting COVID-19 viral RNA and has the potential for mass screening of patients with high viral load.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Joseph Kamau, Elizabeth Ashby, Lindsey Shields, Jennifer Yu, Suzan Murray, Megan Vodzak, Allan Ole Kwallah, Peris Ambala, Dawn Zimmerman
Summary: This study conducted human behavioral surveys among different communities in Kenya that raise livestock, revealing significant variation in reported behaviors among communities and the association between reported ill person in the household and self-reported illnesses. The findings also suggest the importance of targeted studies exploring behavioral variations in animal production contexts for understanding zoonotic pathogen exposure.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)