期刊
JOURNAL OF VIROLOGICAL METHODS
卷 194, 期 1-2, 页码 206-210出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2013.08.028
关键词
LAMP; GFHNV; CyHV-2; Diagnosis
资金
- General Administration of Quality Supervision, Inspection and Quarantine of PR China (AQSIQ) [2013IK043]
Goldfish Haematopoietic Necrosis caused by Cyprinid herpesvirus 2 (CyHV-2) is a severe fish disease with high level of mortality. This is the first study on detection of this disease by loop-mediated isothermal amplification (LAMP). A set of six primers targeting terminase gene (accession no. EU349285.1) was determined after a serial of tests. Detection limit was 1.09 x 10(-4) mu g/mu L, which was superior to conventional PCR and real-time PCR. No cross reaction with 28 other viruses or bacteria commonly found in fish was observed. The application of commercial kit and instrument for the LAMP assay could reduce the risk of cross contamination, which is suitable for detection of infection under field conditions. (C) 2013 Elsevier B.V. All rights reserved.
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