Article
Infectious Diseases
Emmanuelle Robardet, Alexandre Servat, Jonathan Rieder, Evelyne Picard-Meyer, Florence Cliquet
Summary: This study analyzes the rabies diagnosis proficiency test results of 73 laboratories over a 10-year period, finding that conventional RT-PCR offers the highest sensitivity while RTCIT shows the highest specificity. Molecular biology techniques had lower specificity than expected. Regular performance tests have improved laboratory performance over time.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Virology
Diego A. Caraballo, Maria A. Lombardo, Paula Becker, Maria S. Sabio, Cristina Lema, Leila M. Martinez, Fernando J. Beltran, Yu Li, Daniel M. Cisterna
Summary: The study compared the applicability of two widely used qRT-PCR assays for detecting RABV in Argentina, finding that the LN34 qRT-PCR assay successfully detected all variants while the LysGT1 assay failed to detect three bat-related variants. Sequencing revealed that mismatches between the LysGT1 primers and probe sequences with the viral gene sequences can lead to detection failure. It was concluded that the LN34 assay is more effective for RABV detection in Argentina compared to the LysGT1 assay.
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Virology
Lisa Dettinger, Crystal M. Gigante, Maria Sellard, Melanie Seiders, Puja Patel, Lillian A. Orciari, Pamela Yager, James Lute, Annette Regec, Yu Li, Dongxiang Xia
Summary: The Pennsylvania Department of Health Bureau of Laboratories tested 6855 animal samples for rabies using DFA and LN34 RT-qPCR, with a sensitivity of 0.03%. Two samples initially tested negative by DFA but were positive by LN34 RT-qPCR, which were confirmed positive upon re-testing. The levels of rabies virus in different brain structures varied, with higher abundance observed in more caudal brain structures, suggesting early infection.
Article
Veterinary Sciences
Anil Kumar Sharma, Parinita Prashar, Omesh Kumar Bharti, Riyesh Thachamvally, Rashmi Thakur, Naveen Kumar, Karuppusamy Shanmugasundaram, Harisankar Singha, Tarun Kumar Bhattacharya, Baldev Raj Gulati, Shrikrishna Isloor, Ashok Kumar Panda, Jyotsna Sharma, Mankaran Hara, Darryn Knobel
Summary: Rabies in cattle is often under-reported in India due to religious sentiments hindering post-mortem examination. In this study, skin tissue specimens of the nasolabial plate were used as alternative diagnostic specimens for rabies in a suspected cow. Both brain and nasolabial tissue specimens tested positive for rabies using reverse-transcription polymerase chain reaction, indicating high diagnostic sensitivity. Further studies with more nasolabial plate skin specimens are encouraged for post-mortem and ante-mortem diagnosis of rabies in cattle.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Geum-Young Lee, Kyungmin Park, Young-Sun Lee, Ji Hoon Kim, Kwan Soo Byun, Jongwoo Kim, Won-Keun Kim, Jin-Won Song
Summary: This study evaluated the use of multiplex PCR-based nanopore sequencing to obtain whole-genome sequences of HAV for rapid molecular diagnosis of viral genotypes. The results showed high genome coverage rates (90.4-99.5%) of HAV within 8 hours using singleplex nanopore sequencing. TaqMan qPCR demonstrated the ability to detect and quantify multiple HAV genes. This study provides valuable insights for rapid molecular diagnosis during hepatitis A outbreaks and may enhance public health disease surveillance in the hospital and epidemiology field.
Article
Biochemical Research Methods
Giovanni Faggioni, Riccardo De Santis, Filippo Moramarco, Mario Di Donato, Angelo De Domenico, Filippo Molinari, Giancarlo Petralito, Claudia Fortuna, Giulietta Venturi, Giovanni Rezza, Florigio Lista
Summary: Yellow fever is a viral disease that can cause severe hemorrhage, but can be controlled through mass immunization campaigns with a safe and effective vaccine. However, the re-emergence of the yellow fever virus since the 1960s requires rapid and specific viral detection methods for timely control measures. A novel molecular assay with high sensitivity and specificity in detecting yellow fever virus strains has been developed.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Virology
Jaya Garg, Vikramjeet Singh, Pranshu Pandey, Ashish Verma, Manodeep Sen, Anupam Das, Jyotsna Agarwal
Summary: The study evaluated the use of pooled-sample testing strategy during the COVID-19 pandemic, showing that this approach can save substantial resources and time, reducing the number of tests needed by 76% to 93% in low to moderate prevalence settings with group sizes of 5-10 in a population.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Microbiology
Holly R. Hughes, Joan L. Kenney, Brandy J. Russell, Amy J. Lambert
Summary: This article describes the development and validation of a real-time RT-PCR test for the detection of JCV RNA, including evaluation of three primer and probe sets. The study found that both probe sets could be utilized for sensitive screening and specific confirmatory assays.
Article
Multidisciplinary Sciences
Fan Yang, Sijing Yan, Linwei Zhu, Hangping Yao, Dalu Dong, Danna Wu, Nanping Wu, Chunsheng Ye, Haibo Wu
Summary: Avian influenza viruses (AIVs), including highly pathogenic and low pathogenic subtypes, have caused epidemics in birds and posed a health challenge to humans. The low pathogenic subtypes, such as H4, H6, and H10, have covertly spread in domestic poultry and sporadically infected humans, indicating a potential pandemic risk. Therefore, there is an urgent need for a rapid and sensitive diagnostic method to simultaneously detect these AIV subtypes.
Article
Biochemical Research Methods
Amanda Dias Tadeu, Karen Miyuki Asano, Adriana Candido Rodrigues, Helena Beatriz de Carvalho Ruthner Batista, Camila Mosca Barboza, Willian de Oliveira Fahl, Enio Mori, Maria Eduarda Rodrigues Chierato, Keila Iamamoto, Karin Correa Scheffer
Summary: The study showed that cranial cavity lavage is not suitable for viral isolation, but RT-PCR testing of cranial cavity lavage may assist in diagnosis with a sensitivity of 76.8%.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Veterinary Sciences
Yan Zhang, Yuhao Li, Zhixin Guan, Yang Yang, Junjie Zhang, Qing Sun, Beibei Li, Yafeng Qiu, Ke Liu, Donghua Shao, Zhiyong Ma, Jianchao Wei, Peng Li
Summary: A new duplex TaqMan RT-qPCR method for rapid identification and detection of Japanese encephalitis virus (JEV) and Getah virus (GETV) was established in this study. The method showed high sensitivity, extreme specificity, and excellent repeatability, and could be completed in a short time. It provided an effective tool for the detection and differentiation of JEV and GETV.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xi-Wen Jiang, Tao-Sheng Huang, Long Xie, Si-Ze Chen, Shi-Dong Wang, Zhi-Wen Huang, Xin-Yu Li, Wei-Ping Ling
Summary: Acute respiratory infections are common in vulnerable populations and can be caused by various microorganisms. Early detection through rapid pathogen screening is crucial. This study developed a multiplex PCR assay that can simultaneously detect nine respiratory pathogens, offering rapid, sensitive, and contamination-free detection.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Pilailuk Okada, Rome Buathong, Siripaporn Phuygun, Thanutsapa Thanadachakul, Sittiporn Parnmen, Warawan Wongboot, Sunthareeya Waicharoen, Supaporn Wacharapluesadee, Sumonmal Uttayamakul, Apichart Vachlraphan, Malinee Chittaganpitch, Nanthawan Mekha, Noppavan Janejai, Sopon Iamsirithaworn, Raphael T. C. Lee, Sebastian Maurer-Stroh
Article
Public, Environmental & Occupational Health
Ratsameekhae Jongthun, Pasin Hemachudha, Supaporn Wacharapluesadee, Thiravat Hemachudha
Article
Virology
Supaporn Wacharapluesadee, Thongchai Kaewpom, Weenassarin Ampoot, Siriporn Ghai, Worrawat Khamhang, Kanthita Worachotsueptrakun, Phanni Wanthong, Chatchai Nopvichai, Thirawat Supharatpariyakorn, Opass Putcharoen, Leilani Paitoonpong, Gompol Suwanpimolkul, Watsamon Jantarabenjakul, Pasin Hemachudha, Artit Krichphiphat, Rome Buathong, Tanarak Plipat, Thiravat Hemachudha
JOURNAL OF MEDICAL VIROLOGY
(2020)
Article
Ecology
Aingorn Chaiyes, Luis E. Escobar, Emma Willcox, Prateep Duengkae, Warong Suksavate, Pattarawan Watcharaanantapong, Nantachai Pongpattananurak, Supaporn Wacharapluesadee, Thiravat Hemachudha
Article
Immunology
Wisit Prasitsirikul, Krit Pongpirul, Wannarat A. Pongpirul, Nayot Panitantum, Anuttra C. Ratnarathon, Thiravat Hemachudha
EMERGING MICROBES & INFECTIONS
(2020)
Article
Public, Environmental & Occupational Health
Supaporn Wacharapluesadee, Rome Buathong, Sopon Iamsirithawon, Walairat Chaifoo, Teerada Ponpinit, Chanida Ruchisrisarod, Chanikarn Sonpee, Panticha Katasrila, Siriporn Yomrat, Siriporn Ghai, Sunee Sirivichayakul, Pilailuk Okada, Nanthawan Mekha, Opart Karnkawinpong, Sumonmal Uttayamakul, Apichart Vachiraphan, Tanarak Plipat, Thiravat Hemachudha
FRONTIERS IN PUBLIC HEALTH
(2020)
Article
Immunology
Rome Buathong, Walairat Chaifoo, Sopon Iamsirithaworn, Supaporn Wacharapluesadee, Yutthana Joyjinda, Apaporn Rodpan, Weenassarin Ampoot, Opass Putcharoen, Leilani Paitoonpong, Gompol Suwanpimolkul, Watsamon Jantarabenjakul, Sininat Petcharat, Saowalak Bunprakob, Siriporn Ghai, Wisit Prasithsirikul, Anek Mungaomklang, Tanarak Plipat, Thiravat Hemachudha
Summary: The study identified that early COVID-19 patients in Thailand from China carried multiple different virus lineages, providing clear evidence of diverse SARS-CoV-2 clades introduced into the country at an early stage.
MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Virology
Ratanaporn Tangwangvivat, Supaporn Wacharapluesadee, Papassorn Pinyopornpanish, Sininat Petcharat, Suthida Muangnoicharoen Hearn, Nattakarn Thippamom, Chadaporn Phiancharoen, Piyapha Hirunpatrawong, Phattra Duangkaewkart, Ananporn Supataragul, Chadaporn Chaiden, Wiriyachayon Wechsirisan, Nantaporn Wandee, Krongkan Srimuang, Leilani Paitoonpong, Rome Buathong, Chonticha Klungthong, Vichan Pawun, Soawapak Hinjoy, Opass Putcharoen, Sopon Iamsirithaworn
Summary: Wastewater samples from twenty-three sites in the Bangkok Metropolitan Region were collected and tested for the presence of SARS-CoV-2 and its variants. Out of 215 samples, 102 were positive for SARS-CoV-2 RNA. The wastewater variant results were consistent with the clinical specimen data from GISAID. This study demonstrates that wastewater surveillance using multiple signature mutation sites is a cost-effective strategy for monitoring SARS-CoV-2 variants in the community.
Article
Infectious Diseases
Pasin Hemachudha, Sininat Petcharat, Soawapak Hinjoy, Abhinbhen W. Saraya, Thiravat Hemachudha
Summary: The Thai Red Cross, in collaboration with the Ministry of Public Health, conducted encephalitis surveillance and found that enteroviruses have become a major cause of viral encephalitis. The shift in viral neurotropism pattern leading to this change was unexpected.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Public, Environmental & Occupational Health
Supaporn Wacharapluesadee, Siriporn Ghai, Prateep Duengkae, Pattarapol Manee-Orn, Weerapong Thanapongtharm, Abhinbhen W. Saraya, Sangchai Yingsakmongkon, Yutthana Joyjinda, Sanipa Suradhat, Weenassarin Ampoot, Bundit Nuansrichay, Thongchai Kaewpom, Rachod Tantilertcharoen, Apaporn Rodpan, Kachen Wongsathapornchai, Teerada Ponpinit, Rome Buathong, Saowalak Bunprakob, Sudarat Damrongwatanapokin, Chanida Ruchiseesarod, Sininat Petcharat, Wantanee Kalpravidh, Kevin J. Olival, Martha M. Stokes, Thiravat Hemachudha
Summary: Nipah virus (NiV) infection causes encephalitis with a high mortality rate and has outbreak potential in Thailand. The study highlights the importance of cross-sectoral surveillance of NiV in bats, humans, and pigs, and emphasizes the success of a proactive One Health approach in preventing zoonotic disease outbreaks. The genetic similarity between Thai bats and a Bangladeshi patient's NiV strain underscores the need for increased collaboration between human, animal, and wildlife sectors to effectively combat emerging infectious diseases.
ONE HEALTH OUTLOOK
(2021)
Article
Clinical Neurology
Somsak Tiamkao, Anuwat Boonsong, Kamol Saepeung, Narongrit Kasemsap, Metha Apiwattanakul, Narupat Suanprasert, Thiravat Hemachudha, Prakai Pithak, Kittiphit Juntee, Chamnan Waisaen, Supat Madha, Rome Buathong, Kittisak Sawanyawisuth
CASE REPORTS IN NEUROLOGY
(2019)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)