Article
Chemistry, Multidisciplinary
En Ze Sheng, Yu Ting Tan, Yu Xiao Lu, Yue Xiao, Zhen Xi Li
Summary: The study established a TRFIA method with high reliability and accuracy for rapid detection of oxyfluorfen residues, which was validated through correlation tests with real samples. By optimizing the conditions, ic-TRFIA demonstrated superior sensitivity and detection limit compared to dc-TRFIA.
FRONTIERS IN CHEMISTRY
(2021)
Article
Medical Laboratory Technology
Qingqing Wu, Chen Hang, Ting Li, Binrong Wang, Yuan Qin, Yigang Wang, Xiumei Zhou, Pengguo Xia, Huiming Sheng, Pei Huang, Biao Huang
Summary: The study established a simple and rapid immunoassay for determining human serum IgG4 levels using a competition method and magnetic microspheres. The method showed high sensitivity, wide detection range, and good correlation with immunonephelometry, indicating its potential as a useful tool for diagnosing IgG4-RD.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Chemistry, Analytical
Sofia M. Safarian, Pavel A. Kusov, Sergey S. Kosolobov, Oksana Borzenkova, Artem Khakimov, Yuri Kotelevtsev, Vladimir P. Drachev
Summary: Cortisol is a vital steroid hormone that plays a key role in regulating physiological processes and stress responses. The metal-enhanced fluorescence assay allows for continuous real-time monitoring of cortisol levels, a method that has not been demonstrated even in vitro before.
Article
Multidisciplinary Sciences
Xiaojun Wei, Xiaoqin Wang, Zehui Zhang, Yuanyuan Luo, Zixin Wang, Wen Xiong, Piyush K. Jain, John R. Monnier, Hui Wang, Tony Y. Hu, Chuanbing Tang, Helmut Albrecht, Chang Liu
Summary: This study presents a click chemistry amplified nanopore (CAN) assay for ultrasensitive detection of the viral capsid protein p24 in early HIV-1 infection. The CAN assay achieves higher analytical sensitivity than traditional methods and correlates with CD4 count and viral load, suggesting its potential importance in early infection detection and monitoring HIV progression and treatment efficacy. It can also be adapted for detecting other infectious diseases.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Young Hyun Shin, Dong-Eun Kim, Kyung Lee Yu, Chul Min Park, Hong Gi Kim, Kyung-Chang Kim, Songmee Bae, Cheol-Hee Yoon
Summary: Designed a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assay using europium cryptate as a fluorescence donor to investigate the interaction between Tat and TAR RNA for HIV-1 replication. The optimized assay showed specificity and successfully identified two small molecules (460-G06 and 463-H08) capable of inhibiting Tat activity and HIV-1 infection. This assay is suitable for high-throughput screening and the identified compounds may serve as potential molecular scaffolds for new HIV-1 drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Xinchi Wang, Tingting Yang, Xi Chen, Longxiang Fang, Yong Yang, Guoqing Cao, Haitao Zhang, Alex Bogere, Shunlong Meng, Jiazhang Chen, Chao Song
Summary: The study utilized time-resolved fluorescence immunoassay and a portable three-dimensional printing equipment platform combined with smartphones to detect malachite green in aquaculture. The findings demonstrated the reliability of the method and its consistency with the standard method.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Wei Xiao, Jiajie Liang, Ying Zhang, Yan Zhang, Peijun Teng, Dongni Cao, Siyi Zou, Tao Xu, Jianfu Zhao, Yong Tang
Summary: A paper-based cell-counting method utilizing a blocking competition strategy was developed, along with a time-resolved fluorescence-blocking competitive lateral flow immunoassay (TRF-BCLFIA) for CD8 cell counting. This approach can provide an accurate CD8 cell count for whole-blood samples within 30 minutes and shows potential as a cost-effective alternative in community clinics, small hospitals, and low-resource regions where standard flow cytometry-based tools are not readily available.
ANALYTICA CHIMICA ACTA
(2021)
Article
Agriculture, Multidisciplinary
Zi Han, Yi Pan, Shouhui Chen, Xinlin Wei
Summary: A new time-resolved fluorescent immunosensor based on FRET was developed for the rapid detection of cadmium ion. The immunosensor showed a quick response time of 30 minutes, a detection limit of 0.29 ng/mL, and a linear range of 1-500 ng/mL. The accuracy of the immunosensor for cadmium ion detection was further validated through testing tea samples, exhibiting satisfactory recovery rates. This method provides a good model for the rapid and high-throughput detection of heavy metals.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Cheng Lu, Wei Xiao, Yanqiong Su, Xuchao Zhang, Yixiao Chen, Kunjie Lu, Peijun Teng, Jiajie Liang, Huawen Yang, Qifang Song, Yong Tang, Donglin Cao
Summary: In this study, a dyad lateral flow immunoassay strip was developed to detect extracellular vesicles (EVs) for liquid biopsy in diagnosing and monitoring lung cancer. The strip can directly detect trace plasma samples and effectively distinguish cancerous samples from healthy plasma with a high sensitivity and specificity. It also provides a simple and rapid platform for monitoring lung cancer therapy outcomes.
Article
Chemistry, Multidisciplinary
Long Duong Ha, Ki Jun Kim, Seong Jung Kwon, Byoung-Yong Chang, Seongpil Hwang
Summary: This study demonstrates the use of short-time Fourier transform (STFT) and continuous wavelet transform (CWT) electrochemical impedance spectroscopy (EIS) for time-resolved analysis of stochastic collision events of platinum nanoparticles (NPs) onto gold ultramicroelectrode (UME). Enhanced electrocatalytic activity is observed in both chronoamperometry (CA) and EIS. CA provides impact moment and rough estimation of NP size, while quantitative information such as charge transfer resistance (R-ct) relevant to the exchange current density of a single Pt NP is estimated from EIS. CWT analysis of the phase angle parameter proves to be better for NP collision detection in terms of time resolution compared to the STFT method.
Article
Biochemical Research Methods
Zhongyi Xiang, Lele Zhang, Renjing Hu, Xindong Chen, Yuan Qin, Xiumei Zhou, Yigang Wang, Jianfeng Hong, Huqiang Tang, Hongming Fang, Biao Huang
Summary: The study established a highly sensitive HBP-TRFIA, which can effectively assist in diagnosing bacterial or fungal infections in tumor patients. The detection results were consistent with those of fluorescence quantitative immunochromatography, and the plasma HBP concentrations in infected tumor patients were significantly higher than those in uninfected patients.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Zhihua Xu, Mingsong Zhu, Wenxuan Jiang, Tieying Zhang, Mingze Ma, Feng Shi
Summary: This study developed a microsphere immunochromatographic test strip for the detection of folic acid in infant milk powder. The test strip showed rapid, sensitive, and reliable results, making it a practical quantitative detection method.
Article
Veterinary Sciences
Jingneng Wang, Lihua Wang, Hui Zhang, Xinglin Mei, Liangzhu Qiu, Jing Liu, Yongsong Zhou
Summary: Through best management practices, the risk of deoxynivalenol (DON) contamination in food crops and feeds can be effectively managed and mitigated. A DON test strip based on time-resolved fluorescence immunoassay (TRFIA) and a specific DON monoclonal antibody was developed for rapid quantification of DON in food crops and feeds. The strip showed good linearity and high accuracy, making it suitable for on-site and laboratory determination of DON.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Analytical
Xue Liu, Xiuzhai Chen, Xu Zhu, Qing Lin, Xi Pan, Xiaolei Tan, Yongfeng Guo, Jun Qiu, Song Fang
Summary: In this study, ELISA and TRFIA methods were established for the detection and monitoring of quinclorac residue in the environment. The results of both methods were consistent with those of the referenced UPLC-MS/MS method, and they showed good detection performance that met the requirements of quinclorac quantitative determination.
Article
Food Science & Technology
Yongjian Cheng, Bo Xie, Yifan Liang, Xinmei Liu, Haojie Chen, Jiadong Li, Hongtao Lei, Zhili Xiao
Summary: In this study, two PBZ haptens were synthesized and a highly specific monoclonal antibody against PBZ was prepared. A sensitive and rapid TRFMs-LFIA method was established for the qualitative and quantitative analysis of PBZ. The assay showed good performance in spiked wheat and rice samples and was compared to UPLC-MS/MS, demonstrating its potential as an effective strategy for on-site detection of PBZ.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Review
Immunology
Rahul Shukla, Viswanathan Ramasamy, Ravi Kant Rajpoot, Upasana Arora, Ankur Poddar, Richa Ahuja, Hemalatha Beesetti, Sathyamangalam Swaminathan, Navin Khanna
EXPERT REVIEW OF VACCINES
(2019)
Article
Infectious Diseases
Sathyamangalam Swaminathan, Navin Khanna
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Biochemical Research Methods
Teppo Salminen, Etvi Juntunen, Sheikh M. Talha, Kim Pettersson
JOURNAL OF IMMUNOLOGICAL METHODS
(2019)
Article
Immunology
Rajgokul K. Shanmugam, Viswanathan Ramasamy, Rahul Shukla, Upasana Arora, Sathyamangalam Swaminathan, Navin Khanna
PATHOGENS AND DISEASE
(2019)
Article
Multidisciplinary Sciences
Neha Kaushik, Urpo Lamminmaki, Navin Khanna, Gaurav Batra
SCIENTIFIC REPORTS
(2020)
Article
Medicine, General & Internal
Rahul Shukla, Rajgokul K. Shanmugam, Viswanathan Ramasamy, Upasana Arora, Gaurav Batra, Joshua A. Acklin, Florian Krammer, Jean K. Lim, Sathyamangalam Swaminathan, Navin Khanna
Article
Medicine, General & Internal
Rahul Shukla, Hemalatha Beesetti, Julia A. Brown, Richa Ahuja, Viswanathan Ramasamy, Rajgokul K. Shanmugam, Ankur Poddar, Gaurav Batra, Florian Krammer, Jean K. Lim, Sachin Kale, Altaf A. Lal, Sathyamangalam Swaminathan, Navin Khanna
Article
Biology
Sherif Bayoumy, Heidi Hyytia, Janne Leivo, Sheikh M. Talha, Kaisa Huhtinen, Matti Poutanen, Johanna Hynninen, Antti Perheentupa, Urpo Lamminmaki, Kamlesh Gidwani, Kim Pettersson
COMMUNICATIONS BIOLOGY
(2020)
Article
Chemistry, Analytical
Iida Martiskainen, Etvi Juntunen, Teppo Salminen, Karoliina Vuorenpaa, Sherif Bayoumy, Tytti Vuorinen, Navin Khanna, Kim Pettersson, Gaurav Batra, Sheikh M. Talha
Summary: Rapid diagnostic tests are commonly used in low- and middle-income countries for detecting HIV antibodies in remote locations with limited access to central laboratories. Luminescent upconverting nanoparticle reporters have been shown to help overcome the risk of misinterpretation in these tests. The developed UCNP-LFA demonstrates the applicability of UCNPs for the detection of anti-HIV antibodies, with potential for automated result interpretation, archiving, and data transfer from decentralized locations.
Article
Virology
Hemalatha Beesetti, Sathyamangalam Swaminathan
Summary: In this study, a HuAd5 vector encoding shRNA was shown to effectively suppress the replication of all four prototypic DENV serotypes in Vero cells and human macrophages. The HuAd5 vector-mediated DENV suppression in macrophages was found to be dependent on the presence of anti-HuAd5 antibodies, suggesting implications for the specific targeting of HuAd5 vector-mediated antiviral RNA interference therapy to macrophages.
Letter
Medicine, General & Internal
Sathyamangalam Swaminathan, Navin Khanna
NEW ENGLAND JOURNAL OF MEDICINE
(2020)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)