期刊
JOURNAL OF VIROLOGICAL METHODS
卷 163, 期 2, 页码 498-504出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2009.11.010
关键词
SUMO; Ubc9; Influenza; NS1A; IRES; SUMOylation
资金
- American Heart Association [0765137Y]
- National Institutes of Allergy and Infectious Diseases (NIAID) [1SC2AI081377-01]
- National Institute of General Medical Sciences (NIGMS)
- National Institutes of Health (NIH) [5G12RR008124]
- University of Texas at El Paso
The cellular SUMOylation system affects the function of numerous viral proteins. Hence, the identification of novel viral targets for the Small Ubiquitin-like Modifier (SUMO) is key to our understanding of virus-host interactions. The data obtained in this study demonstrate that the non-structural influenza A viral protein NS1A is an authentic SUMO target through the use of a dicistronic expression plasmid containing SUMO (the modifier) and Ubc9 (the SUMO-conjugating enzyme) separated by an internal Ribosomal Entry Site (IRES). This dual expression plasmid produces a robust increase in cellular SUMOylation, therefore facilitating the characterization of cellular and viral SUMO targets. The identification of NS1A as a bona fide SUMO target suggests, for the first time, a role for SUMOylation during influenza virus infection. (C) 2009 Elsevier B.V. All rights reserved.
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