期刊
JOURNAL OF VIROLOGICAL METHODS
卷 159, 期 2, 页码 185-193出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2009.03.016
关键词
HIV; Drug resistance; TAQAS; Quality assessment; Genotyping
资金
- The TREAT Asia Quality Assurance Scheme is an initiative of TREAT Asia
- amfAR
- The Foundation for AIDS Research
- Dutch Ministry of Foreign Affairs through a partnership with Stichting Aids Fonds
- Australian Government Department of Health and Ageing
The TREAT Asia (Therapeutics, Research, Education, and AIDS Training in Asia) Network is building capacity for Human Immunodeficiency Virus Type-1 (HIV-1) drug resistance testing in the region. The objective of the TREAT Asia Quality Assessment Scheme-designated TAQAS-is to standardize HIV-1 genotypic resistance testing (HIV genotyping) among laboratories to permit rigorous comparison of results from different clinics and testing centres. TAQAS has evaluated three panels of HIV-1-positive plasma from clinical material or low-passage, culture supernatant for up to 10 Asian laboratories. Laboratory participants used their standard protocols to perform HIV genotyping. Assessment was in comparison to a target genotype derived from all participants and the reference laboratory's result. Agreement between most participants at the edited nucleotide sequence level was high (>98%). Most participants performed to the reference laboratory standard in detection of drug resistance mutations (DRMs). However, there was variation in the detection of nucleotide mixtures (0-83%) and a significant correlation with the detection of DRMs (p<0.01). Interpretation of antiretroviral resistance showed similar to 70% agreement among participants when different interpretation systems were used but >90% agreement with a common interpretation system, within the Stanford University Drug Resistance Database. Using the principles of external quality assessment and a reference laboratory, TAQAS has demonstrated high quality HIV genotyping results from Asian laboratories. (C) 2009 Elsevier B.V. All rights reserved.
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