期刊
JOURNAL OF VIROLOGICAL METHODS
卷 156, 期 1-2, 页码 162-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2008.10.020
关键词
Influenza virus; M1 protein; Fixation method; Nuclear domain 10
资金
- Ministry of Education, Culture, Sports, Science and Technology
The distribution of the matrix (M1) protein of influenza virus in infected cells was examined using immunostaining. The fixation method influenced strongly the immunofluorescence pattern of the M1 protein. The M1 protein was distributed uniformly in both the cytoplasm and in nuclei when cells that had been infected with virus were fixed with paraformaldehyde. In cells that had been fixed with methanol, however, nuclear dots of the M1 protein were clearly visible. The dots were evident at 8 h post-inoculation. Up to 6 h post-inoculation, only a diffuse distribution of the M1 protein was observed. The dots were co-localized with promyelocytic leukemia (PML) protein, a major component of nuclear domain 10 (ND10), also called PML oncogenic domains (PODs) or PML-nuclear bodies (NBs). These results indicate that the nuclear dots of the M1 protein in cells that had been fixed with methanol are not artifacts of the fixation method. Furthermore, methanol fixation is preferred for localization of the influenza M1 protein in nuclei using immunostaining. (C) 2008 Elsevier B.V. All rights reserved.
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