期刊
JOURNAL OF VIROLOGICAL METHODS
卷 158, 期 1-2, 页码 24-29出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2009.01.007
关键词
Hepatocellular carcinoma; Hepatitis B virus; Biomarker; Locked nucleic acid
资金
- NIH [R01 CA125642]
The 1762T/1764A double mutation of the hepatitis B virus (HBV) basal core promoter has been suggested to be a potential biomarker for hepatocellular carcinoma (HCC) among individuals with chronic HBV infection. In this study, a real-time PCR assay is established using the hybridization probes and an oligonucleotide clamp containing locked nucleic acids (LNAs). The LNA-containing oligonucleotide clamp specific for the wild type HBV is able to suppress the amplification of the wild type HBV templates. In addition, the clamp can inhibit the binding of the WT templates to the fluorescence probes thereby suppress the wild type HBV signals during the melting curve analyses. These effects facilitated the detection of HBV double mutation in the presence of 3000-fold excess of the wild type genome. Thus PCR amplification coupled with the melting curve analyses provides a quick. simple, and highly sensitive tool for the detection of this HBV double mutation. (c) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据