Article
Biochemistry & Molecular Biology
Hye Hyeon Yun, Soon Young Jung, Bong Woo Park, Ji Seung Ko, Kyunghyun Yoo, Jiyoung Yeo, Hong Lim Kim, Hun Jun Park, Ho Joong Youn, Jeong Hwa Lee
Summary: The study developed tamoxifen-inducible cardiomyocyte-specific BIS-KO mice and found that disruption of the Bis gene led to impaired heart function and heart failure. These mice may serve as a useful model for developing therapeutic interventions for dilated cardiomyopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Kirsten Warcup, Alex S. Torson, George D. Yocum
Summary: "Research on Sex Determination Methods of Colorado Potato Beetles" used publicly available RNA-seq data to select 5 candidate genes for sex-specific markers in adult Colorado potato beetles. The study confirmed that these 5 marker candidates exhibit a sex-specific expression pattern and can be used as PCR markers for sex determination. This method allows for simple sex detection using cDNA as the template and assigning sex to RNA-seq samples post hoc.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Daan van Beek, Job Verdonschot, Kasper Derks, Han Brunner, Theo M. M. de Kok, Ilja C. W. Arts, Stephane Heymans, Martina Kutmon, Michiel Adriaens
Summary: This study developed an analysis pipeline for detecting allele-specific expression, which can be used to study cis-regulatory variation and its impact on complex genetic disorders such as dilated cardiomyopathy. The pipeline was applied to a cohort of 87 well-phenotyped patients and revealed an overrepresentation of known disease-associated genes. Additionally, novel genes that have not been identified through conventional methods were also discovered. The pipeline provides various analysis tools and visualizations for candidate gene identification and biological insight.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Xiujie Gao, Jindou Hao, Lin Yu, Yan Cao, Jianfang Liang, Juan Han, Rong Zou, Xike Zhou, Peihui Liu
Summary: The outbreak of the COVID-19 epidemic has emphasized the need for rapid and accurate detection methods for enterovirus. This study established an ultra-fast RT-PCR equipment to evaluate a testing method for enterovirus nucleic acids. The results showed that the method achieved fast and accurate detection within 24 minutes, with high sensitivity and good repeatability.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biochemical Research Methods
Trisha R. Barnard, Alex B. Wang, Selena M. Sagan
Summary: The conventional RT-qPCR protocols cannot accurately distinguish different viral RNA species during infection. The developed method reduces non-specific products and enables multiplex detection of positive-strand and negative-strand ZIKV RNA simultaneously.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Cardiac & Cardiovascular Systems
Zsofia Gregor, Anna Reka Kiss, Kinga Grebur, Liliana Erzsebet Szabo, Bela Merkely, Hajnalka Vago, Andrea Szucs
Summary: This study compared the left ventricular MRI characteristics of dilated cardiomyopathy and left ventricular noncompaction patients, finding similarities in volumetric, global strain parameters, and rotational patterns, but higher TPMi values and significantly lower apical circumferential strains in LVNC patients.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Immunology
Signe Ravn Warncke, Charlotte Rohde Knudsen
Summary: This article reviews the methods for genome detection in viruses and discusses the process of replication of positive-sense, single-stranded RNA viruses. Future detection tools that may utilize specific strand RNA detection techniques are proposed.
Review
Immunology
Nora M. Chapman
Summary: Enteroviruses have been found to persist in cell cultures and in vivo through a novel mechanism involving the deletion of a part of the genome, resulting in mutant populations. Studies have shown significant differences between these mutant populations, termed TD enteroviruses, and canonical enteroviruses, with the TD enteroviruses becoming dominant in quiescent cell populations.
Article
Medicine, General & Internal
Joanna Petryka-Mazurkiewicz, Karolina Kryczka, Lukasz Mazurkiewicz, Barbara Milosz-Wieczorek, Mateusz Spiewak, Magdalena Marczak, Jan Henzel, Jacek Grzybowski, Marcin Demkow, Zofia Dzielinska
Summary: Using cardiovascular magnetic resonance (CMR) imaging, subtle differences were found between peripartum cardiomyopathy (PPCM) and dilated cardiomyopathy (DCM), with the main variations being observed in the performance of the right atrium (RA) and left ventricular (LV) global longitudinal strain.
Article
Virology
Alexis Bouin, Michelle N. Vu, Ali Al-Hakeem, Genevieve P. Tran, Joseph H. C. Nguyen, Bert L. Semler
Summary: Group B enteroviruses, including coxsackievirus B3 (CVB3), can persistently infect cardiac tissue and cause dilated cardiomyopathy. 5' terminal deletions of viral genomic RNAs have been detected in persistently infected patients, and our study showed that these deletions decreased the levels of viral proteinase activity and synthesis of viral protein and RNA. However, a cooperative interaction was observed when full-length and terminally deleted forms were cotransfected into cardiomyocytes, leading to increased viral production.
JOURNAL OF VIROLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Jesse Gammons, Mohamed Trebak, Salvatore Mancarella
Summary: Orai3 plays a critical role in the heart, mediating Ca2+ signaling essential for maintaining sarcomere integrity and proper mitochondrial function in adult mammalian cardiomyocytes. Loss of Orai3 in mice results in ventricular dysfunction progressing to dilated cardiomyopathy and heart failure, with significant changes in myocardial Ca2+ cycling and expression of critical proteins involved in Ca2+ clearance mechanisms.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Nanoscience & Nanotechnology
Zhongjie Fei, Ping Liu, Chu Cheng, Rongbin Wei, Pengfeng Xiao, Youfa Zhang
Summary: Solvent-responsive magnetic beads (responsive MBs) were developed for SARS-CoV-2 nucleic acid detection, improving the absorption and desorption efficiencies of nucleic acids. These responsive MBs showed better performance and sensitivity compared to commercial magnetic beads, and were successfully applied in various NA-based technologies.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qiao Xu, Ke Liu, Jingjing Jin, Xinfeng Zhang
Summary: A cascaded strand displacement reaction system was designed for detecting low abundant biomarker protein in serum. The system converted a protein recognition event into DNA catalyst output, improving CHA reaction efficiency and CL signal.
MICROCHEMICAL JOURNAL
(2021)
Article
Virology
Tomo Daidoji, Ronald Enrique Morales Vargas, Katsuro Hagiwara, Yasuha Arai, Yohei Watanabe, Keisuke Nishioka, Fumi Murakoshi, Kotaro Garan, Hiroki Sadakane, Takaaki Nakaya
Summary: Flaviviruses are arboviruses carried by arthropods and/or vertebrates, and a novel group called insect-specific flaviviruses has been identified. While not directly harmful to human health, these insect-specific flaviviruses can affect the replication of other human-pathogenic flaviviruses within co-infected mosquito cells. Understanding the prevalence of flaviviruses in arthropods is crucial to assessing disease risks and ecology. The development of a universal primer for PCR-based detection of flaviviruses has shown promising results in amplifying viral sequences and detecting genomes in infected cells and mosquito homogenates, providing a valuable tool for public health and safety.
Article
Medicine, General & Internal
Marjorie Canu, Lea Margerit, Ismail Mekhdoul, Alexis Broisat, Laurent Riou, Loic Djaileb, Clemence Charlon, Adrien Jankowski, Michele Magnesa, Caroline Augier, Stephanie Marliere, Muriel Salvat, Charlotte Casset, Marion Maurin, Carole Saunier, Daniel Fagret, Catherine Ghezzi, Gerald Vanzetto, Gilles Barone-Rochette
Summary: The study aimed to investigate the prognostic impact of coronary atherosclerotic burden (CAB) in patients with non-ischemic dilated cardiomyopathy (dCMP). The results indicated that high CAB may be a new prognostic factor in dCMP patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)