Article
Chemistry, Analytical
Hanh An Nguyen, Heewon Choi, Nae Yoon Lee
Summary: In this study, a rotatable paper device integrating the loop-mediated isothermal amplification (RT-LAMP) technique and naked-eye readout was developed for the detection of infectious pathogens. The device utilized hydroxyl radicals to decolorize a food additive called carmoisine, and the color change of the solution indicated the presence of target DNA. The rotatable paper device successfully detected SARS-CoV-2, SARS-CoV, and Enterococcus faecium in milk within 70 minutes using only visual observation. The device offers a portable and low-cost tool for pathogen detection in resource-limited environments.
Article
Biotechnology & Applied Microbiology
Yi Wang, Xiaoxia Wang, Hai Chen, Limei Han, Licheng Wang, Ting Chen, Sha Li, Huan Li, Yuanli Li, Zhengkun Li, Xiaoying Fu, Shaojin Chen, Mei Xing, Jun Tai, Xiong Zhu
Summary: A novel detection platform called rRT-LAMP was developed for the diagnosis of COVID-19, which integrates reverse transcription, LAMP amplification, restriction endonuclease cleavage, and real-time fluorescence detection. This method showed high sensitivity with no cross-reactivity and can diagnose COVID-19 in just 35 minutes. It has the potential to be a valuable tool for detecting SARS-CoV-2 infection and controlling the COVID-19 epidemic.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biology
Igor P. Oscorbin, Lidiya M. Novikova, Maxim L. Filipenko
Summary: Reverse transcriptases (RTs) are crucial enzymes used in RNA-related studies. However, the performance of different RTs in reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is not well-studied. This study compared various M-MuLV RTs in RT-LAMP and found that mutated enzymes performed better overall but were more sensitive to high concentrations of NaCl. These findings are important for developing novel LAMP-based diagnostic tests.
Article
Chemistry, Analytical
Maud Savonnet, Mathilde Aubret, Patricia Laurent, Yoann Roupioz, Myriam Cubizolles, Arnaud Buhot
Summary: This paper presents a theoretical framework to analyze the kinetics of the second stage of LAMP, which can help optimize LAMP performance by reducing the number of experiments needed to find the best parameters.
Article
Virology
Olympia E. Anastasiou, Caroline Holtkamp, Miriam Schafer, Frieda Schon, Anna Maria Eis-Huebinger, Andi Krumbholz
Summary: This study examined the reliability of a commercial LAMP assay in directly detecting SARS-CoV-2 genomes in respiratory samples without nucleic acid extraction. The assay exhibited excellent specificity but moderate sensitivity, making it a promising tool for rapid on-site testing or verification of reactive rapid tests.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Biochemical Research Methods
Wenhao Zhao, Shuhua Wu, Linlin Du, Tingfang Li, Zhaobang Cheng, Yijun Zhou, Yinghua Ji
Summary: A new RT-LAMP assay was developed for rapid and accurate detection of TMGMV in pepper, which was shown to be more sensitive and specific than the traditional RT-PCR method. This method has the potential to be an affordable diagnostic tool for monitoring TMGMV infection in field samples.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Infectious Diseases
Haruka Abe, Yuri Ushijima, Rodrigue Bikangui, Georgelin Nguema Ondo, Ayong Moure, Yoric Yali-Assy-Oyamli, Rokusuke Yoshikawa, Bertrand Lell, Ayola A. Adegnika, Jiro Yasuda
Summary: This study conducted a long-term validation of the RT-LAMP assay and demonstrated its applicability for clinical diagnosis of COVID-19 in resource-limited settings in Africa, particularly in rural areas of Gabon.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Agriculture, Multidisciplinary
Yubao Zhang, Ruoyu Wang, Zhongkui Xie, Yajun Wang, Xia Zhao, Yang Liu, Qin Zhou
Summary: The study introduces a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for detecting Japanese hornwort mosaic virus (JHMV) in angelica plants. This RT-LAMP assay showed higher sensitivity and lower detection limit compared to conventional RT-PCR, proving to be accurate and efficient for JHMV diagnosis. Field surveys using RT-LAMP demonstrated improved disease management and forecasting capabilities for JHMV in Angelica sinensis in China.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Microbiology
John Webster, Monica A. Kehoe, Elisse Nogarotto, Linda Falconer, Nerida Jane Donovan, Toni A. Chapman
Summary: Swift and accurate diagnostics are essential for responding to pathogen incursions. Current assays for citrus bacterial canker lack specificity when testing Australian samples, prompting the development of a more specific detection tool. Genomic comparisons and validation against isolates were utilized to develop an improved diagnostic method with 100% corroboration, enhancing the ability for effective identification of citrus bacterial canker.
Review
Agronomy
Amit Ahuja, Vishal Singh Somvanshi
Summary: The review highlights the utility of Loop-mediated isothermal amplification (LAMP) for rapid detection and diagnosis of plant-parasitic nematodes (PPNs), showcasing its advantages over PCR-based assays in terms of speed and sensitivity. The LAMP assays target specific gene regions and are capable of detecting nematode DNA in various sources, making it a popular choice for on-site detection and diagnosis of PPNs.
Article
Nanoscience & Nanotechnology
Zhengkang Li, Yuwei Di, Xiaoyan Song, Yanqi Wu, Yingye Feng, Xinqiang Zhang, Caiping Gong, Guanghua Li
Summary: In this study, a quantitative detection method for NoV GII was established, and the RT-LAMP detection system was found to be a viable alternative to RT-PCR for qualitative detection of NoV. Compared to RT-PCR, RT-LAMP offers shorter detection time and lower technical requirements.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Agronomy
Mi-Chi Yao, Peng-Xiang Zhu, Qin-Qin Zhang, Qi-Ping Zhang, Zhao-Lin Ji, Feng Zhu
Summary: Wisteria, a decorative climbing plant, is susceptible to the destructive WVMV. Establishing an efficient RT-LAMP detection method for WVMV can help reduce economic losses through effective prevention and control.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
