Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Food Science & Technology
Damkerng Bundidamorn, Wannakarn Supawasit, Sudsai Trevanich
Summary: A simple Taqman(R) probe based mRT-PCR method was developed to detect three major foodborne pathogenic bacteria with high sensitivity, specificity and accuracy.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Daire Cantillon, Adam P. Roberts
Summary: This study developed a protocol to evaluate virus detection in near source tracking (NST) sampling using Pepper Mild Mottle Virus (PMMoV) as a proxy. Two novel TaqMan RT-PCR assays were developed to detect PMMoV genomic 5' region and capsid associated gene. The results showed consistent and comparable detection of PMMoV in ten independent samples across two NST samplers, regardless of the presence of pepper homogenate.
Article
Virology
Kareem E. Hassan, Ann Kathrin Ahrens, Ahmed Ali, Magdy F. El-Kady, Hafez M. Hafez, Thomas C. Mettenleiter, Martin Beer, Timm Harder
Summary: This article introduces a real-time RT-PCR method for comprehensive subtyping of hemagglutinin and neuraminidase subtypes of AIV by introducing a total of 24 arrayed RT-qPCR reactions. The technique not only allows for diagnosis of AIV infections but also includes detection of other viruses. The method demonstrates good inclusivity and exclusivity in validation runs and is suitable for influenza A virus in avian hosts.
Article
Chemistry, Analytical
Jixue Weng, Nan Sheng, Runyuan Wang, Shuo Liang, Chen Wang, Xue Bai, Guohua Zhou, Bingjie Zou, Qinxin Song
Summary: Cervical cancer is a significant cause of death in women, with HPV typing being crucial for diagnosis and control. The Multi-Vision method, utilizing closed-tube PCR, offers high specificity and sensitivity for typing various HPV types, making it a useful tool for HPV detection in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
M. A. Motalib Hossain, Siti Aimi Sarah Zainal Abidin, Aishah Bujang, Mohd Nasir Taib, Suresh Sagadevan, Mohd Rafie Johan, Nina Naquiah Ahmad Nizar
Summary: Meat and meat products are highly sought after globally for their high-quality protein and nutrients, but the industry faces challenges such as food fraud, mislabeling, and zoonotic diseases. Quantitative detection of the species origin using methods like PCR is crucial for food control. This review focuses on the design, development, and applications of TaqMan probe-based mqPCR techniques for authenticating meat products, providing a reference for academia and industry to improve existing assays.
Article
Virology
Chenyan Wang, Bo Hou, Guoqing Shao, Chunhe Wan
Summary: The study explored the potential and reliability of the real-time TaqMan analysis method for detecting and distinguishing nVarIBDV genotype from non-nVarIBDV, especially live vaccine strains. This method accurately identified nVarIBDV and non-nVarIBDV strains, without cross-reactivity with other viruses. The one-step real-time TaqMan RT-PCR method provides a rapid, sensitive, specific, and simple approach for detecting nVarIBDV infections and is a useful clinical diagnostic tool.
Article
Medicine, Legal
Qinrui Yang, Baonian Liu, Yuxiang Zhou, Yining Yao, Zhihan Zhou, Hui Li, Chengchen Shao, Kuan Sun, Hongmei Xu, Qiqun Tang, Yiwen Shen, Jianhui Xie
Summary: This study demonstrated the practical value of mRNA profiling in body fluid identification, using a one-step multiplex reverse transcription PCR strategy with 18 tissue-specific biomarkers targeting various body fluids. The results showed that this method could provide reliable and economical analysis of tissue-specific biomarkers for the discrimination of body fluid origin.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Microbiology
Simone Scherrer, Roger Stephan
Summary: The novel TaqMan 5-plex real-time PCR method developed using a combination of LNA- and MGB-conjugated DNA probes enables identification and differentiation between the four main pathogenic Brachyspira species in swine. The assay is highly sensitive and specific, easy to implement in veterinary diagnostic laboratories, and allows rapid differentiation between different Brachyspira species.
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Dan Huang, Hui Deng, Juan Zhou, Guan A. Wang, Qian Lei, Chen Guo, Wanting Peng, Peng Liang, Chenlan Shen, Binwu Ying, Weimin Li, Feng Li
Summary: PANDA technology eliminates the interference caused by high concentrations of wild-type sequences and enables the ultrasensitive detection of cancer mutations with low VAFs. Compared to digital PCR, PANDA has fewer false negatives and lower costs. It also offers multiplex testing and has wide applications in analyzing rare mutations in diverse diseases.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Veterinary Sciences
Nahed Yehia, Fatma Eldemery, Abdel-Satar Arafa, Ahmed Abd El Wahed, Ahmed El Sanousi, Manfred Weidmann, Mohamed Shalaby
Summary: The H9N2 subtype of avian influenza A virus is circulating globally, causing severe economic losses. The developed H9N2 RT-RPA assay is highly sensitive and specific, allowing for rapid and accurate diagnosis of H9 aIAV infection.
VETERINARY SCIENCES
(2021)
Article
Multidisciplinary Sciences
Fan Yang, Sijing Yan, Linwei Zhu, Hangping Yao, Dalu Dong, Danna Wu, Nanping Wu, Chunsheng Ye, Haibo Wu
Summary: Avian influenza viruses (AIVs), including highly pathogenic and low pathogenic subtypes, have caused epidemics in birds and posed a health challenge to humans. The low pathogenic subtypes, such as H4, H6, and H10, have covertly spread in domestic poultry and sporadically infected humans, indicating a potential pandemic risk. Therefore, there is an urgent need for a rapid and sensitive diagnostic method to simultaneously detect these AIV subtypes.
Article
Virology
Preeti Chhabra, Hannah Browne, Thalia Huynh, Marta Diez-Valcarce, Leslie Barclay, Margaret N. Kosek, Tahmeed Ahmed, Maria Renee Lopez, Chao-Yang Pan, Jan Vinje
Summary: This study validated a one-step conventional RT-PCR assay for dual typing of GI and GII noroviruses, successfully genotyping most known genotypes infecting humans. The use of broadly reactive genotyping assays greatly enhances the exchange of standardized genotype data globally to monitor trends in genotype diversity, which is essential for vaccine development and impact measurement.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Virology
Jiratchaya Puenpa, Patthaya Rattanakomol, Nutsada Saengdao, Jira Chansaenroj, Ritthideach Yorsaeng, Kamol Suwannakarn, Somchai Thanasitthichai, Sompong Vongpunsawad, Yong Poovorawan
Summary: This study investigated the molecular characteristics and evolution of SARS-CoV-2 in Thailand from 2020 to 2022. The results showed that different variants, including B.1.36.16, B.1.1.7, B.1.617.2, and B.1.1.529, caused outbreaks in different waves. Continued molecular surveillance is crucial for monitoring emerging variants.
ARCHIVES OF VIROLOGY
(2023)
Article
Virology
Fajar Budi Lestari, Kirkvich Chandranoi, Watchaporn Chuchaona, Sompong Vongpunsawad, Yong Poovorawan
Summary: A 2-year-old indoor female Siamese cat with bloody mucoid diarrhea was diagnosed with rotavirus group A infection by real-time reverse transcription polymerase chain reaction (RT-PCR). Genetic analysis revealed a unique G3P[9] genome constellation in this feline rotavirus strain, indicating genetic reassortment had occurred between human feline-like and human bovine-like rotaviruses.
ARCHIVES OF VIROLOGY
(2023)
Article
Virology
Jiratchaya Puenpa, Nutsada Saengdao, Nongkanok Khanarat, Sumeth Korkong, Jira Chansaenroj, Ritthideach Yorsaeng, Nasamon Wanlapakorn, Yong Poovorawan
Summary: This study investigated the molecular epidemiological trends, evolutionary dynamics, and recombination characteristics of CV-A6-associated HFMD in Thailand between 2019 and 2022. The results revealed that CV-A6 was the predominant genotype and identified four recombinant forms. These findings are important for the development of vaccines to prevent the spread of CV-A6 infection.
Article
Immunology
Passakorn Wanchaijiraboon, Panot Sainamthip, Nattaya Teeyapun, Sutima Luangdilok, Yong Poovorawan, Nasamon Wanlapakorn, Suebpong Tanasanvimon, Virote Sriuranpong, Thiti Susiriwatananont, Nicha Zungsontiporn, Nussara Pakvisal
Summary: We evaluated the adverse events of the booster doses of BNT162b2 and mRNA-1273 vaccines in solid cancer patients previously vaccinated with ChAdOx1 and CoronaVac. There was no significant difference in the incidence and severity of adverse events between the two vaccines, except for a higher occurrence of tenderness at the injection site after mRNA-1273 vaccination.
Article
Immunology
Nasamon Wanlapakorn, Napa Pruetarat, Nasiri Sarawanangkoor, Kanchana Phanphanit, Donchida Srimuan, Thaksaporn Thatsanathorn, Thanunrat Thongmee, Nawarat Posuwan, Yong Poovorawan
Summary: This study compared the immunogenicity of the Shan-5 vaccine with the optional Quinvaxem and Infanrix-hexa vaccines. The results showed that the immunogenicity of the Shan-5 vaccine was similar to the hexavalent vaccine, but higher than the Quinvaxem vaccine.
Article
Virology
Jiratchaya Puenpa, Vorthon Sawaswong, Pattaraporn Nimsamer, Sunchai Payungporn, Patthaya Rattanakomol, Nutsada Saengdao, Jira Chansaenroj, Ritthideach Yorsaeng, Kamol Suwannakarn, Yong Poovorawan
Summary: This study conducted complete genome sequencing of 210 samples to investigate the SARS-CoV-2 lineages circulating in Thailand and their evolutionary history, revealing multiple lineage introductions before the emergence of the omicron variant. The evolutionary rate for the spike gene of SARS-CoV-2 was estimated, and certain predominant mutations in the ORF3a gene were identified during the Thailand outbreaks. Complete genome sequencing can enhance the prediction of future variant changes and is crucial for vaccine strain development.
Article
Immunology
Teeraporn C. Bureerug, Sitthichai Kanokudom, Nungruthai Suntronwong, Ritthideach Yorsaeng, Suvichada Assawakosri, Thanunrat Thongmee, Yong Poovorawan
Summary: The study aimed to evaluate the level of anti-S1 IgA in the serum of participants immunized with different COVID-19 vaccination regimens. The findings showed that heterologous boosters, especially after priming with an inactivated vaccine, elicited higher IgA levels than homologous boosters. Vaccination with SV/SV/PF produced the highest IgA level among all the immunization regimens after either two, three, or four doses.
Article
Immunology
Sitthichai Kanokudom, Jira Chansaenroj, Nungruthai Suntronwong, Suvichada Assawakosri, Ritthideach Yorsaeng, Pornjarim Nilyanimit, Ratchadawan Aeemjinda, Nongkanok Khanarat, Preeyaporn Vichaiwattana, Sirapa Klinfueng, Thanunrat Thongmee, Donchida Srimuan, Thaksaporn Thatsanathorn, Natthinee Sudhinaraset, Nasamon Wanlapakorn, Sittisak Honsawek, Yong Poovorawan
Summary: The aim of this study is to investigate the reactogenicity and immunogenicity of the fourth dose using monovalent mRNA vaccines after different three-dose regimens and to compare the 30 μg BNT162b2 and 50 μg mRNA-1273 vaccines. A total of 292 healthy adults received BNT162b2 or mRNA-1273 and the reactogenicity was mild to moderate and well tolerated. The study found that both BNT162b2 and mRNA-1273 vaccines can be used as a fourth booster dose for individuals who were previously immunized with any prior three-dose mix-and-match COVID-19 vaccine regimens.
Review
Gastroenterology & Hepatology
Palittiya Sintusek, Nasamon Wanlapakorn, Yong Poovorawan
Summary: Mother-to-child transmission (MTCT) of hepatitis B virus (HBV) is a significant global health burden, but it can be prevented with antiviral treatment, immunoprophylaxis, and timely vaccination. Cesarean section and breastfeeding avoidance may be options for high-risk mothers, but more evidence is needed. Screening and effective strategies should be considered for worldwide application.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2023)
Article
Virology
Patthaya Rattanakomol, Sarawut Khongwichit, Watchaporn Chuchaona, Sompong Vongpunsawad, Yong Poovorawan
Summary: The complete genome sequences of two Thai SFTSV strains were reported, which showed a close relationship with previously reported strains from central China. The observation of amino acid residue differences between Thai strains suggests separate introductions of different variants into the region.
ARCHIVES OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Payuda Hansoongnern, Pornpitra Pratedrat, Pornjarim Nilyanimit, Rujipat Wasitthankasem, Nawarat Posuwan, Nasamon Wanlapakorn, Kanchanok Kodchakorn, Prachya Kongtawelert, Napaporn Pimsing, Yong Poovorawan
Summary: Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis and can lead to cirrhosis and hepatocellular carcinoma. The detection of HCV RNA is the standard method for diagnosis and monitoring of antiviral treatment. A study investigated the correlation between HCV RNA and HCVcAg levels, as well as the impact of amino acid sequence heterogeneity on HCVcAg quantification. The findings showed a strong positive correlation between HCV RNA and HCVcAg, but samples with certain genotypes and amino acid substitutions exhibited lower HCVcAg levels than expected based on HCV RNA values.
Article
Immunology
Anussara Prayongrat, Patjaya Noppaving, Thitiporn Chobarporn, Natthinee Sudhinaraset, Nattaya Teeyapun, Nussara Pakvisal, Watsamon Jantarabenjakul, Jiratchaya Sophonphan, Chawalit Lertbutsayanukul, Yong Poovorawan
Summary: Diminished immune response was observed in cancer patients receiving radiotherapy after COVID-19 vaccination. This study evaluated the immune response and safety profile in 53 cancer patients undergoing radiotherapy who received the COVID-19 vaccine. The results showed that the immunogenicity was fair after the first dose, but achieved a higher seroconversion rate after the second dose. However, the immunologic response in these patients was still lower than in healthy individuals, suggesting the need for additional preventive strategies.
Article
Immunology
Sitthichai Kanokudom, Jira Chansaenroj, Suvichada Assawakosri, Nungruthai Suntronwong, Ritthideach Yorsaeng, Lakkhana Wongsrisang, Ratchadawan Aeemjinda, Preeyaporn Vichaiwattana, Sirapa Klinfueng, Thaksaporn Thatsanathorn, Sittisak Honsawek, Yong Poovorawan
Summary: This study investigated the impact of hybrid immunity on antibody responses in participants receiving various doses of the COVID-19 vaccine. It found that a higher number of vaccine doses was associated with a lower infection rate. Hybrid immunity displayed higher antibody levels and durability compared to vaccination alone.
Article
Pediatrics
Palittiya Sintusek, Siriporn Khunsri, Jira Chansaenroj, Thanunrat Thongmee, Yong Poovorawan
Summary: This study investigated the prevalence and impact of hepatitis E virus (HEV) infection in liver transplanted children with transaminitis. The results showed a high prevalence of HEV infection in these children, with two patients showing evidence of chronic infection. The findings suggest the importance of investigating HEV infection and providing appropriate management for liver transplanted children with persistent transaminitis.
PEDIATRIC TRANSPLANTATION
(2023)
Article
Medicine, Research & Experimental
Pornpitra Pratedrat, Duangnapa Intharasongkroh, Jira Chansaenroj, Preeyaporn Vichaiwattana, Donchida Srimuan, Thaksaporn Thatsanatorn, Sirapa Klinfueng, Pornjarim Nilyanimit, Chintana Chirathaworn, Pawinee Kupatawintu, Dootchai Chaiwanichsiri, Nasamon Wanlapakorn, Yong Poovorawan
Summary: This study investigated the relationship between the immunological response and the efficacy of convalescent plasma therapy in COVID-19 patients. The study found that convalescent plasma therapy led to higher antibody levels and changes in biochemical markers and cytokine levels. The therapy also demonstrated an immunoregulatory effect on cytokine parameters.
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)