期刊
JOURNAL OF VIROLOGICAL METHODS
卷 148, 期 1-2, 页码 197-204出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2007.11.012
关键词
Varicella Zoster Virus; bacterial artificial chromosome; deletion mutagenesis; bioluminescence
资金
- NIAID NIH HHS [R01 AI055381-04, R01 AI055381, AI050709-01, R01 AI050709-05, R01 AI050709] Funding Source: Medline
Varicella Zoster Virus (VZV) is the causative agent for both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large DNA genome, encoding 70 unique open reading frames (ORFs). The functions of the majority of these ORFs remain unknown. Recently, the full-length VZV (P-Oka strain) genome was cloned as a VZV bacteria artificial chromosome (BAC) and additionally a firefly luciferase cassette was inserted to generate a novel luciferase VZV BAC. In this study, a highly efficient protocol has been developed exploiting the new luciferase VZV BAC system to rapidly isolate and characterize VZV ORF deletion mutants by growth curve analysis in cell culture. Published by Elsevier B.V.
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