Article
Agriculture, Dairy & Animal Science
David G. Horvath, Zsolt Abonyi-Toth, Marton Papp, Attila Marcell Szasz, Till Ruemenapf, Christian Knecht, Heinrich Kreutzmann, Andrea Ladinig, Gyula Balka
Summary: This study used QuPath software for digital image analysis to count and classify endometrial inflammatory cells, demonstrating the effectiveness and applicability of the method. It suggests that similar procedures can provide new knowledge about reproductive disorders caused by porcine reproductive and respiratory syndrome virus-1 in the future.
Article
Virology
Yulin Xu, Youwen Zhang, Shaohua Sun, Jia Luo, Sen Jiang, Jiajia Zhang, Xueliang Liu, Qi Shao, Qi Cao, Wanglong Zheng, Nanhua Chen, Francois Meurens, Jianzhong Zhu
Summary: The cGAS-STING signal plays an important antiviral role in PRRSV infection, as demonstrated by the suppression of infection with stable expression of STING or stimulation of cGAS-STING signaling, while the knockout of STING gene increases the level of infection.
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Fangshu Ye, David H. Baum, Luis G. Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: This study aimed to evaluate the consistency of endogenous reference gene expression and their interpretation in diagnostic specimens. Results showed that the reference gene can accurately monitor sample quality in swine specimens tested for PRRSV. Failure to detect the reference gene may indicate abnormalities in the sample or testing procedures.
VETERINARY SCIENCES
(2023)
Article
Computer Science, Artificial Intelligence
Qing Zhang, Jin Zhou, Lei Zhu, Wei Sun, Chunxia Xiao, Wei-Shi Zheng
Summary: In this paper, an unsupervised framework for image decomposition is proposed, which can effectively learn the decomposition from a single natural image. By promoting the internal self-similarity of the reflectance component, the framework decomposes the image into reflectance and shading, and trains the network using a novel loss function. Experimental results on benchmark datasets demonstrate the superiority of the proposed method.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2022)
Article
Biochemistry & Molecular Biology
Tabata Almeida Silva, Rodrigo Barbosa Aguiar, Marcelo Mori, Gabriel Esquitini Machado, Barbara Hamaguchi, Marcelo Ferreira Marcondes Machado, Jane Zveiter Moraes
Summary: This study evaluated the potential of an anti-bevacizumab idiotype single-chain variable fragment (scFv) in a gene immunization strategy and found that it has the ability to induce an immune response containing VEGF-binding antibodies.
Article
Veterinary Sciences
Hua Zhang, Zhigang Cao, Panpan Sun, Ajab Khan, Jianhua Guo, Yaogui Sun, Xiuju Yu, Kuohai Fan, Wei Yin, E. Li, Na Sun, Hongquan Li
Summary: This study optimized a component formula from traditional Chinese medicine compounds for anti-PRRSV activity and found a synergistic effect in vitro.
BMC VETERINARY RESEARCH
(2022)
Article
Computer Science, Information Systems
Annalisa Franco, Antonio Magnani, Davide Maltoni, Dario Maio, Leonardo Odorisio, Andrea De Maria
Summary: Face image quality estimation is an open issue, especially for electronic ID documents. This study aims to define a quality score indicator for high-quality images and confirms the effectiveness of the proposed approach.
Article
Engineering, Mechanical
Mohammad Khodadadi Dehkordi, Amin Taraghi Osguei, Iman Khamoushi, Ekaterina Pavlovskaia, Marian Wiercigroch
Summary: In this study, a mathematical model of the anti stick-slip tool (ASST) is developed for the first time, considering the internal friction on the helical spline and the pre-load of the internal spring. Four distinct states of the coupled rotational and axial movements of the tool are proposed, providing a better understanding of the mechanics of the ASST. Parametric studies and modeling are conducted, laying the foundation for further research on the operation of the tool.
INTERNATIONAL JOURNAL OF MECHANICAL SCIENCES
(2022)
Article
Computer Science, Artificial Intelligence
Jingting Zhong, Cihui Yang, Xingmiao Xu, Wuzhida Bao, Jianyong Guo
Summary: In this paper, a novel algorithm is proposed to locate and recognize ID card information. By implementing strategies such as image rectification, boundary detection, and information location, the algorithm effectively addresses the issues of time-consuming, labor-intensive, and inaccurate traditional approaches.
INTERNATIONAL JOURNAL OF PATTERN RECOGNITION AND ARTIFICIAL INTELLIGENCE
(2022)
Article
Immunology
Yulin Xu, Mengxue Ye, Youwen Zhang, Shaohua Sun, Jia Luo, Sen Jiang, Jiajia Zhang, Xueliang Liu, Qi Shao, Qi Cao, Wanglong Zheng, Francois Meurens, Nanhua Chen, Jianzhong Zhu
Summary: This study identified TRIF and MAVS as the most effective adaptors in conferring anti-PRRSV activity, indicating the involvement of multiple pattern recognition receptor signaling pathways in anti-PRRSV innate immunity.
Article
Hematology
Kenichi Ogiwara, Shoko Furukawa, Sho Shinohara, Yuka Tabuchi, Nobuo Arai, Mariko Noguchi-Sasaki, Tetsuhiro Soeda, Midori Shima, Keiji Nogami
Summary: Anti-emicizumab mAbs can cancel the effect of emicizumab and accurately measure procoagulant and anticoagulant factor activity.
Article
Oncology
Paulina M. Wojnarowicz, Marta Garcia Escolano, Yun-Han Huang, Bina Desai, Yvette Chin, Riddhi Shah, Sijia Xu, Saurabh Yadav, Sergey Yaklichkin, Ouathek Ouerfelli, Rajesh Kumar Soni, John Philip, David C. Montrose, John H. Healey, Vinagolu K. Rajasekhar, William A. Garland, Jeremy Ratiu, Yuan Zhuang, Larry Norton, Neal Rosen, Ronald C. Hendrickson, Xi Kathy Zhou, Antonio Iavarone, Joan Massague, Andrew J. Dannenberg, Anna Lasorella, Robert Benezra
Summary: AGX51 targets ID proteins in cancer cells, inhibiting cell growth and enhancing ROS production, showing potential therapeutic effects for multiple cancers.
Article
Computer Science, Information Systems
Valerio Marsocci, Lorenzo Lastilla
Summary: This study focuses on the similarity among works of art based on human poses, utilizing pose clustering and deep learning frameworks. Two alternative distance functions are proposed to measure similarity, along with a modified K-Medians algorithm for clustering similar poses. The proposed approach shows higher robustness to outliers compared to popular clustering strategies.
ISPRS INTERNATIONAL JOURNAL OF GEO-INFORMATION
(2021)
Article
Oncology
Janik Puttemans, Benoit Stijlemans, Marleen Keyaerts, Sam Vander Meeren, Wim Renmans, Karel Fostier, Pieterjan Debie, Heleen Hanssens, Magdalena Rodak, Marek Pruszynski, Kim De Veirman, Karin Vanderkerken, Tony Lahoutte, Alfred Morgenstern, Frank Bruchertseifer, Nick Devoogdt, Matthias D'Huyvetter
Summary: Multiple myeloma is an incurable cancer, and the lack of effective treatment options has led to disease recurrence. However, patient-specific membrane-expressed paraproteins could hold the key to targeting residual cells responsible for disease recurrence. In this study, radiolabeled anti-idiotypic camelid single-domain antibody fragments (sdAbs) were used as tumor-restrictive vehicles and showed potential for personalized medicine in multiple myeloma patients.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Computer Science, Information Systems
Lianli Gao, Qike Zhao, Junchen Zhu, Sitong Su, Lechao Cheng, Lei Zhao
Summary: SIMGAN is a novel GAN-based approach for manipulating image attributes through natural language descriptions. It consists of External Structuring (ExST) and Internal Structuring (InST) components, which significantly outperform existing methods both quantitatively and qualitatively in experiments. The efficiency and accuracy of editing text-relevant image attributes are greatly improved compared to state-of-the-art methods.
IEEE TRANSACTIONS ON MULTIMEDIA
(2023)
Article
Biochemistry & Molecular Biology
Pan Wang, Peiyang Ding, Qiang Wei, Hongliang Liu, Yunchao Liu, Qingmei Li, Yunrui Xing, Ge Li, Enmin Zhou, Gaiping Zhang
Summary: This study reports the generation and characterization of monoclonal antibodies against the spike receptor-binding domain (RBD) of MERS-CoV. Two novel linear epitopes were identified on the outermost surface of RBD. The findings may contribute to a better understanding of RBD's function and the development of diagnostic methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Yani Sun, Wenlong Yan, Xu Chen, Qianqian Liu, Pinpin Ji, Jiahong Zhu, Lili Gai, Xiaoxuan Li, Jiakai Zhao, Lu Zhang, Hao Zhang, Baoyuan Liu, En-Min Zhou, Qin Zhao
Summary: This study analyzed the antigenic cross-reactivity among human, swine, rabbit, and avian hepatitis E virus (HEV) capsid proteins and found immunodominant epitopes in different species. These findings provide insights for the development of serological assays for diagnosing HEV infections in various animal species.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Mengnan Fan, Yuhang Luo, Beibei Zhang, Jiaxi Wang, Tianxiang Chen, Baoyuan Liu, Yani Sun, Yuchen Nan, Julian A. Hiscox, Qin Zhao, En-Min Zhou
Summary: The study confirmed the direct interaction between HEV and CDC42, and identified different CDC42 downstream signaling pathways used by non-enveloped and quasi-enveloped HEV during infection. These results provide new insights into the life cycle of HEV in host cells and offer a new drug design target for combating HEV infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Beibei Zhang, Guanggang Qu, Yuchen Nan, En-Min Zhou
Summary: The study found that infertility caused by ZP3 vaccination is associated with ovarian oxidative stress and follicle loss. Antioxidant stress drugs can reduce oxidative stress and apoptosis levels, restore follicle numbers, and improve fertility.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Baoyuan Liu, Yiyang Chen, Meimei Zhang, Tianxiang Chen, Yuan Zhang, DanBaZha Xi, Shixuan Xu, Qin Zhao, En-Min Zhou
Summary: This study confirms the presence of HEV infection in laboratory Bama miniature pigs in China and demonstrates that these pigs can be experimentally infected with HEV. Screening of laboratory pigs for HEV is important to prevent zoonotic infections.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Liangliang Li, Weiyao Sun, Qifan Hu, Tongtong Wang, Guang Zhu, Qin Zhao, En-Min Zhou
Summary: This study found that MYH9 is an important factor for PRRSV entry into target cells and accelerates the infection process by binding with PRRSV. This finding contributes to the development of new therapeutic agents to control PRRSV infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jiakai Zhao, Hao Zhang, Lu Zhang, Qiang Zhang, Ning Zhou, Taofeng Du, Qin Zhao, En-Min Zhou, Yongkun Du, Yani Sun
Summary: This study investigated the molecular variations of parvovirus in cats, dogs, minks, and raccoon dogs from the eastern region of Shandong province, China. The results showed cross-species infection between the feline panleukopenia virus (FPV), mink enteritis virus (MEV), and raccoon dog parvovirus (RDPV), suggesting continuous evolution of the virus in areas with a high density of economic animal trading/breeding.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Wenqi Fan, Pinpin Ji, Xuwen Sun, Min Kong, Ning Zhou, Qiang Zhang, Ying Wang, Qianqian Liu, Xiaoxuan Li, En-Min Zhou, Qin Zhao, Yani Sun
Summary: In this study, 16 nanobodies against the NDV nucleocapsid protein were screened, and 8 were shown to induce an immune response. Nb18, Nb30, and Nb88 significantly inhibited the replication of different genotypes of NDV strains in cells. These nanobodies provide new tools for researching the functions of NDV NP protein and may be used as a novel strategy for designing drugs against NDV infection in chickens.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pinpin Ji, Kun Wang, Lu Zhang, Zhenda Yan, Min Kong, Xuwen Sun, Qiang Zhang, Ning Zhou, Baoyuan Liu, En-Min Zhou, Yani Sun, Xinjie Wang, Qin Zhao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Tianxiang Chen, Baoyuan Liu, Yiyang Chen, Xueting Wang, Meimei Zhang, Xukun Dang, Qin Zhao, En-Min Zhou
Summary: A rapid and reliable competitive ELISA (cELISA) was developed to detect avian HEV antibodies, which showed good specificity. The epitope recognized by Nb49 was located in aa 593-604 of the avian HEV capsid protein, and the peptide TFPS in aa 601-604 was essential for binding.
Article
Microbiology
Yiyang Chen, Xueting Wang, Meimei Zhang, Jinyao Li, Xueyan Gao, Yuchen Nan, Qin Zhao, En-Min Zhou, Baoyuan Liu
Summary: In this study, nanobodies with high affinity were screened and shown to block HEV infection, and they exhibited good protective effects in an animal model.
FRONTIERS IN MICROBIOLOGY
(2022)
Correction
Virology
Lizhen Wang, Lu Zhang, Baicheng Huang, Kuokuo Li, Gaopeng Hou, Qin Zhao, Chunyan Wu, Yuchen Nan, Taofeng Du, Yang Mu, Jixun Lan, Hongying Chen, En-Min Zhou
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Yiyang Chen, Meimei Zhang, Tianxiang Chen, Jiaxi Wang, Qin Zhao, En-Min Zhou, Baoyuan Liu
Summary: A novel nanobody-based competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect Hepatitis E virus (HEV) antibodies in different species. The cELISA showed high specificity, sensitivity, and convenience, making it a promising technique for serological evaluation and diagnosis of HEV infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Jiakai Zhao, Jiahong Zhu, Ying Wang, Mengting Yang, Qiang Zhang, Chong Zhang, Yuchen Nan, En-Min Zhou, Yani Sun, Qin Zhao
Summary: A simple nanobody-based competitive ELISA method has been developed in this study to detect the presence of antibodies against ASFV in pig sera. The method is characterized by its low cost, easy production, and 100% agreement with a commercial ELISA kit.
Article
Microbiology
Beibei Zhang, Mengnan Fan, Jie Fan, Yuhang Luo, Jie Wang, Yajing Wang, Baoyuan Liu, Yani Sun, Qin Zhao, Julian A. Hiscox, Yuchen Nan, En-Min Zhou
Summary: This study identified the activation of Rap1b during avian HEV infection, which induced the membrane recruitment of Talin-1. This activation further activated the Integrin-FAK-Cofilin cascade, leading to F-actin rearrangement and membrane remodeling, potentially facilitating the internalization of CaHEV virions.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
