Article
Fisheries
Trung Hieu Pham, Shreesha Rao, Ta-Chih Cheng, Pei-Chi Wang, Shih-Chu Chen
Summary: Vaccination is the most effective method for preventing the dangerous pathogen Photobacterium damselae subsp. piscicida in aquaculture. A study found that the recombinant protein Fba showed good immunoprotective effects against photobacteriosis in Asian sea bass, with significantly lower bacterial load and higher survival rate.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Aki Nishihara, Natsuki Morimoto, Takechiyo Sumiyoshi, Shinya Yasumoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
Summary: The Ivy gene in the plasmid of Pdp may help the bacteria evade lysozyme-mediated lysis during interaction with an animal host. This study found that Ivy-Pdp is a temperature- and pH-stable lysozyme inhibitor, and it inhibits the lytic activity of lysozyme involved in host biophylaxis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Antonio Vallecillos, Elena Chaves-Pozo, Marta Arizcun, Ruben Perez, Juan M. Afonso, Concepcion Berbel, Jaume Perez-Sanchez, Emilio Maria-Dolores, Eva Armero
Summary: A challenge test for Photobacterium damselae subsp. piscicida resistance was conducted in two populations of gilthead seabream, revealing moderate heritabilities for body weight and disease resistance, and low heritability for days to death. Genetic correlations were high and positive between body weight and disease survival, and tended to be positive with immune markers. Selective breeding programs may consider incorporating immune markers like peroxidase activity, along with performance traits, to breed disease-resistant fish.
Article
Food Science & Technology
Ines Lua Freitas, Alexandra Teixeira, Ines Loureiro, Johnny Lisboa, Aurelia Saraiva, Nuno Miguel Simoes dos Santos, Ana do Vale
Summary: Photobacterium damselae subsp. piscicida (Phdp) is a Gram-negative bacterium that infects various marine fish species. Its virulence factor, AIP56, induces apoptosis of sea bass phagocytes during infection. This study found that sea bream is resistant to AIP56 toxicity due to inefficient internalization of the toxin. In contrast, sea bass is more susceptible to AIP56-induced lethality. These findings highlight the different effects of AIP56 in sea bass and sea bream.
Article
Immunology
Feng-Jie Su, Meei-Mei Chen
Summary: The oral biofilm vaccine demonstrated a protective effect against Photobacterium damselae subsp. damselae, increasing phagocytosis and improving immune response in infected fish, resulting in reduced cumulative mortality rate.
Article
Fisheries
Jiasong Xie, Han Mei, Shan Jin, Lingfei Bu, Xinyi Wang, Chunlin Wang, Qingsong Zhao, Rongrong Ma, Suming Zhou
Summary: In May 2017, a massive mortality of mud crab Scylla paramamosain occurred in a farm in Sanmen, Zhejiang Province, China, caused by Photobacterium damselae subsp. damselae infection. The QX175062 isolate was lethal to mud crabs with LD(50) value of 3.63 x 10(1) CFU/g, highlighting the importance of developing rapid diagnostic methods and prevention measures for economic losses in mud crab aquaculture due to P. damselae subsp. damselae infection.
Article
Fisheries
Trung Hieu Pham, Ta-Chih Cheng, Pei-Chi Wang, Shih-Chu Chen
Summary: HSP33 is a potential candidate for vaccine development against Photobacterium damselae subsp. piscicida infection, with high expression levels of immune-related genes and antibody titres observed in the rHSP33 group. The sera of this group also displayed high bactericidal activity against Phdp.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Saqr Abushattal, Ana Vences, Carlos R. Osorio
Summary: The unstable plasmid pPHDPT3, encoding a type III secretion system, is a major virulence factor of the marine bacterium Photobacterium damselae subsp. piscicida (Pdp). However, pPHDPT3 undergoes curing upon in vitro cultivation, resulting in a significant reduction in Pdp's virulence for fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Teixeira, Ines Loureiro, Johnny Lisboa, Pedro N. Oliveira, Jorge E. Azevedo, Nuno M. S. dos Santos, Ana do Vale
Summary: Phdp is a Gram-negative fish pathogen that causes significant economic losses in aquaculture. This study found that Phdp secretes large amounts of OMVs during in vitro culture and infection, which protect Phdp cells from fish antimicrobial peptides. Vaccination with OMVs induced the production of anti-Phdp antibodies and provided partial protection against infection in sea bass.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Zoology
Daniella-Mari White, Michail-Aggelos Valsamidis, Vasileios Bakopoulos
Summary: This study aimed to investigate the sensitivity of common octopus to bacterial infection, assess the activation of hemocytes and their phagocytic activity after infection, and examine the relationship between sensitivity, phagocytic activity, and temperature, route of infection, and pathogen.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2022)
Article
Genetics & Heredity
Laura Baseggio, Oleksandra Silayeva, Nicky Buller, Matt Landos, Jan Englestadter, Andrew C. Barnes
Summary: Despite recent technological advances, complete assembly of multi-chromosome genomes of Vibrionaceae remains challenging. This study successfully sequenced, closed, and curated the genomes of two strains of Photobacterium damselae subsp. piscicida isolated in Australia using a combination of long Oxford Nanopore MinION reads and short Illumina reads. Phylogenetic analysis revealed closer relationship of Australian strains to Asian and American strains rather than to European ones. Host specialization and adaptive evolution to fish in P. damselae subsp. piscicida are ongoing processes mediated by transposable elements and plasmids. Identification of potential virulence determinants like the chorismate mutase gene and the transfer-messenger RNA gene ssrA provides new insights into the evolution of this fish pathogen.
MICROBIAL GENOMICS
(2021)
Article
Biochemistry & Molecular Biology
Paulo Santos, Diogo Peixoto, Ines Ferreira, Ricardo Passos, Pedro Pires, Marco Simoes, Pedro Pousao-Ferreira, Teresa Baptista, Benjamin Costas
Summary: Photobacteriosis is a bacterial disease that affects various marine species globally, leading to significant economic losses. Despite studies on pathogen virulence and resistance, information on host defense mechanisms post-infection remains limited. This study focused on innate immune responses of farmed juvenile gilthead seabream after infection with Photobacterium damselae subsp. piscicida, revealing marked changes in blood parameters and gene expression related to inflammation and phagocytic activity. Further research on different fish species and pathogens could help identify health biomarkers for disease prevention in aquaculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Alba V. Barca, Ana Vences, Mateus S. Terceti, Ana do Vale, Carlos R. Osorio
Summary: This study investigated the transcriptional response of the facultative marine pathogen Photobacterium damselae subsp. damselae to changes in salinity. It was found that low salinity conditions triggered a virulence gene expression profile, while growth at high salinity upregulated genes involved in energy production and metabolism. This study enhances our understanding of the salinity-responsive adaptations of a generalist marine pathogen.
Article
Fisheries
Yongxiang Yu, Yingeng Wang, Meijie Liao, Linni Shi, Yongjie Li, Bin Li, Xiaojun Rong, Chunyuan Wang, Zheng Zhang
Summary: This study investigated the spleen transcriptome response of black rockfish infected with Photobacterium damselae subsp. damselae, revealing the involvement of immune-related genes in the pathogenesis process over time. The expression profiles of immune-related genes were found to correlate with disease progression, with more immune-related signal pathways and genes involved in the early stage of infection. The findings provide valuable insights into the molecular responses of black rockfish to photobacteriosis.
AQUACULTURE RESEARCH
(2022)
Article
Microbiology
Johnny Lisboa, Cassilda Pereira, Aline Rifflet, Juan Ayala, Mateus S. Terceti, Alba V. Barca, Ines Rodrigues, Pedro Jose Barbosa Pereira, Carlos R. Osorio, Francisco Garcia-del Portillo, Ivo Gomperts Boneca, Ana do Vale, Nuno M. S. dos Santos
Summary: Peptidoglycan (PG) is a crucial component of the bacterial cell wall, and the enzyme PnpA produced by Phdp can cleave PG from competing bacteria, aiding Phdp in gaining a competitive advantage.
Article
Fisheries
Sean J. Monaghan, Diana Chee, Alexandra Adams, Sven M. Bergmann, Shin M. Chong, Jing Chen, Kim D. Thompson
Summary: The study found that only 31% of koi from PCR-positive KHV fish groups were seropositive in ELISA testing. Furthermore, over 34% of seropositive but PCR-negative fish had titres of >1/400. These results highlight the concerns related to carp populations that have been screened for KHV using molecular methods alone and emphasize the need for serology to accompany molecular testing in carp for this notifiable virus.
Review
Fisheries
Matthijs Metselaar, Massimo Orioles, Marco Galeotti, Alexandra Adams, Kim D. Thompson
Summary: Red mark syndrome (RMS) is a skin condition affecting rainbow trout aquaculture, first reported in the United States and subsequently spreading to other parts of the world. It causes economic loss to the industry and has been associated with two pathogens, although the exact cause is still unknown.
Article
Biotechnology & Applied Microbiology
Ae Rin Lee, Seong Bin Park, Si Won Kim, Jae Wook Jung, Jin Hong Chun, Jaesung Kim, Young Rim Kim, Jassy Mary S. Lazarte, Ho Bin Jang, Kim D. Thompson, Myunghwan Jung, Min Woo Ha, Tae Sung Jung
Summary: The study demonstrates that membrane vesicles (MVs) from Gram-positive bacteria are capable of transferring beta-lactam antibiotic-resistant substances to antibiotic-sensitive Gram-negative bacteria, resulting in increased resistance to beta-lactam antibiotics.MVs play a role in transferring substances from Gram-positive to Gram-negative bacteria, shown by the release of MVs from RC85-T that were able to protect beta-lactam-susceptible bacteria from beta-lactam antibiotics.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Fisheries
R. Hoare, K. Shahin, K. McLean, A. Adams, K. D. Thompson
JOURNAL OF FISH DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Apoorva M. Kulkarni, Shraddha Parate, Gihwan Lee, Yongseong Kim, Tae Sung Jung, Keun Woo Lee, Min Woo Ha
Summary: The study utilized computational techniques to validate the potential IL2R alpha binding properties of three polyphenol stilbenes from fenugreek, demonstrating stable interactions with IL2R alpha through molecular docking and molecular dynamics simulations. Rhaponticin showed slightly better binding free energy and all stilbenes followed ADMET rules. Further in vitro IL2R alpha binding studies, especially on rhaponticin, are supported by this computational study.
Article
Immunology
Thao Thu Mai, Pattanapon Kayansamruaj, Chayanit Soontara, Pattarawit Kerddee, Dinh-Hung Nguyen, Saengchan Senapin, Janina Z. Costa, Jorge del-Pozo, Kim D. Thompson, Channarong Rodkhum, Ha Thanh Dong
Summary: Immunization of tilapia broodstock with TiLV vaccines can induce antibody response and transfer maternal antibodies to fertilized eggs and larvae, providing protection against TiLV. HKV appears to be more promising than FKV for maternal vaccination.
Article
Fisheries
Ana Herrero, Oswaldo Palenzuela, Hamish Rodger, Chris Matthews, Mar Marcos-Lopez, James E. Bron, Mark P. Dagleish, Kim D. Thompson
Summary: The microsporidian D. lepeophtherii is difficult to detect in tissue sections, and this study developed an ISH protocol specific for D. lepeophtherii, which showed higher sensitivity compared to other methods.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Seong Bin Park, Yon Kyoung Park, Min Woo Ha, Kim D. Thompson, Tae Sung Jung
Summary: Pathogenic typing, molecular typing, and antimicrobial susceptibility tests were conducted on 75 isolates of E. coli from stool samples of patients suffering from foodborne diseases in Busan, South Korea. The isolates showed high resistance to certain antibiotics but high susceptibility to others, indicating a high level of heterogeneity between the E. coli isolates.
Article
Microbiology
Ana Herrero, Hamish Rodger, Adam D. Hayward, Chris Cousens, James E. Bron, Mark P. Dagleish, Kim D. Thompson
Summary: Complex gill disorder (CGD) is an important condition in Atlantic salmon aquaculture, but the roles of the putative aetiological agents in the pathogenesis are uncertain. This study shows that the loads of certain pathogens are significantly associated with the severity of CGD, and water temperature and management factors also have an impact on gill lesions.
Review
Fisheries
Bei Wang, Kim D. Thompson, Eakapol Wangkahart, Jidapa Yamkasem, Melba G. Bondad-Reantaso, Puntanat Tattiyapong, Jianchang Jian, Win Surachetpong
Summary: In recent years, emerging and re-emerging diseases have had a significant economic impact on the tilapia industry. Without effective management and appropriate biosecurity measures, infectious diseases will continue to challenge the sustainability of global tilapia aquaculture. Enhancing immune response and improving husbandry management can help reduce the impact of diseases.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Anurak Bunnoy, Kim D. Thompson, Patcharapong Thangsunan, Putita Chokmangmeepisarn, Teerapong Yata, Nopadon Pirarat, Sirikorn Kitiyodom, Pattanapong Thangsunan, Pimwarang Sukkarun, Pochara Prukbenjakul, Naphat Panthukumphol, Manami Morishita, Prapansak Srisapoome, Channarong Rodkhum
Summary: A new bivalent mucoadhesive nanovaccine was developed for immersion vaccination of tilapia against francisellosis and columnaris disease. It has the potential to improve innate and adaptive immunity in vaccinated fish, increasing survival rates.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Dennis Berbulla Bela-ong, Kim D. Thompson, Hyoung Jun Kim, Seong Bin Park, Tae Sung Jung
Summary: Fish diseases caused by viruses pose a significant threat to aquaculture. Understanding the immune mechanisms involved in antiviral defense is crucial for developing sustainable disease protection strategies. This review focuses on the role of CD4+ T cells in fish immune responses to viral infections and immunomodulation. Studies have shown that CD4+ T cells in fish exhibit similar responses to mammalian Th cells, including proliferation and expression of key cytokines, transcription factors, and effector molecules. Gaps in our understanding of teleost CD4+ T cell responses are discussed, and the development of novel tools and approaches to study these responses is suggested.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Immunology
Kim D. Thompson, Channarong Rodkhum, Anurak Bunnoy, Patcharapong Thangsunan, Sirikorn Kitiyodom, Pimwarang Sukkarun, Jakarwan Yostawornkul, Teerapong Yata, Nopadon Pirarat
Summary: Tilapia is extensively farmed and provides an important source of food and income for low-income families. However, disease outbreaks caused by bacteria and viruses have increased with the intensification of farming systems. Nanoparticle-based vaccines offer potential solutions to improve vaccine acceptance and uptake among tilapia farmers.
Review
Food Science & Technology
Stacia Stetkiewicz, Rachel A. Norman, Edward Hugh Allison, Neil L. Andrew, Gulshan Ara, Gill Banner-Stevens, Ben Belton, Malcolm Beveridge, Jessica R. Bogard, Simon R. Bush, Pete Coffee, Margaret Crumlish, Peter Edwards, Mahmoud Eltholth, Lynne Falconer, Joao G. Ferreira, Angus Garrett, Iain Gatward, Faruk U. Islam, Alexander M. Kaminski, Marian Kjellevold, Froukje Kruijssen, William Leschen, Abdullah-Al Mamun, Bruce McAdam, Richard Newton, Birgitte Krogh-Poulsen, Alexandra Pounds, Belinda Richardson, Nanna Roos, Elin Roeoes, Andrea Schapper, Tori Spence-McConnell, Sharon K. Suri, Shakuntala Haraksingh Thilsted, Kim D. Thompson, Michael F. Tlusty, Max Fredrik Troell, Raffaele Vignola, James A. Young, Wenbo Zhang, David C. Little
Summary: This review assesses the representation of seafood in recent food security literature and finds that seafood is under-researched compared to terrestrial production systems. The study also reveals a lack of research on the interaction between terrestrial and aquatic systems. Therefore, there is a need for more attention to be given to the role of seafood in global food security and for its integration into interdisciplinary research.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
