Article
Microbiology
Xiangyun Wu, Jiayi Liu, Jiawei Feng, Muhammad Abu Bakr Shabbir, Yali Feng, Rui Guo, Meifang Zhou, Sulin Hou, Guiqiang Wang, Haihong Hao, Guyue Cheng, Yulian Wang
Summary: This study investigated the antimicrobial resistance pattern and virulence profile of K. pneumoniae in five dairy farms in Hubei, China. The results showed that K. pneumoniae has epidemiological importance in food-producing animals and the emergence of multidrug-resistant K. pneumoniae poses a potential threat to food safety and public health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Diego Borin Nobrega, Aline Parolin Calarga, Leandro Costa Nascimento, Carla Gasparotto Chande Vasconcelos, Eliane Martins de Lima, Helio Langoni, Marcelo Brocchi
Summary: This observational study investigated the phenotypic and genotypic patterns of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds. The study found higher antimicrobial resistance rates in isolates from intramammary infections, and identified specific genetic variants and ESBL genes among extended-spectrum beta-lactamase producers. Common resistance was detected against tetracycline, streptomycin, and sulfamethoxazole-trimethoprim, with tetA, sul2, and floR being the most frequently observed AMR genes in resistant isolates.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Jia Cheng, Man Zhou, Diego B. Nobrega, Herman W. Barkema, Siyu Xu, Mengyue Li, John P. Kastelic, Yuxiang Shi, Bo Han, Jian Gao
Summary: In this study, genetic diversity of Klebsiella pneumoniae causing mastitis was investigated on two large Chinese dairy farms using repetitive element sequence-based PCR. The pathogen was found to have high genetic diversity in milk samples from cows with subclinical and clinical mastitis, as well as environmental and extramammary samples. This suggests that K. pneumoniae is an opportunistic and environmental pathogen causing outbreaks of clinical mastitis on these farms.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Shaodong Fu, Chen Wen, Zhenglei Wang, Yawei Qiu, Yihao Zhang, Jiakun Zuo, Yuanyuan Xu, Xiangan Han, Zhenhua Luo, Wei Chen, Jinfeng Miao
Summary: This study investigated the distribution and antimicrobial resistance genes of Klebsiella pneumoniae in dairy cattle farms in China. It found a high prevalence of hypermucoviscous strains in one of the farms. Using multilocus sequence typing, six sequence types were identified that could potentially spread in specific regions. Interestingly, the ST43 sequence type was observed for the first time in bovine isolates.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Dairy & Animal Science
Jia Cheng, Man Zhou, Diego B. Nobrega, Zhijun Cao, Jingyue Yang, Chunyan Zhu, Bo Han, Jian Gao
Summary: This study investigated the virulence profile and capsular types of Klebsiella pneumoniae isolates from different sources on two dairy farms in China. It found that K57 was the dominant capsular type and beta-D-lacZ was widely distributed across all sources, with no significant association between virulence genes and the severity of mastitis.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Yuxiang Shi, Wenpeng Zhao, Gang Liu, Tariq Ali, Peng Chen, Yongxia Liu, John P. Kastelic, Bo Han, Jian Gao
Summary: The bacteriophages CM8-1 and SJT-2 were found to mitigate K. pneumoniae-induced inflammation in bMECs, reducing bacterial adhesion and invasion, cytotoxicity, and inflammatory responses. The study suggests the potential of these bacteriophages for treating mastitis in cows should be evaluated in clinical trials.
BMC VETERINARY RESEARCH
(2021)
Article
Agronomy
Wendi Cao, Yi Xu, Yicai Huang, Tianle Xu
Summary: Recent investigations have shown an increase in mastitis caused by environmental pathogens, specifically K. pneumoniae. This research examined the epidemiology of pathogens in milk samples from four farms in Jiangsu Province. The results revealed the presence of 16 pathogens in 186 positive milk samples. K. pneumoniae exhibited low sensitivity to penicillin and amoxicillin, but high sensitivity to gentamicin and piperacillin. Additionally, the study found a strong biofilm-forming ability among K. pneumoniae isolates, which was related to tetracycline resistance. The detection rate of the ESBL-resistant gene was 100%. The study provides important insights into the prevalence of mastitis-derived pathogens and resistance genes, highlighting the need for rational drug use and disease prevention.
Article
Immunology
Qianhui Ma, Zhaoxuan Zhu, Yue Liu, Jia Wang, Zihao Pan, Huochun Yao, Jiale Ma
Summary: A hypervirulent K2 isolate of Klebsiella pneumoniae was identified in milk samples, potentially linked to an infection incident in children, highlighting the zoonotic potential of bovine mastitis Kp as a foodborne pathogen. Further analysis revealed the presence of pathogenic K1, K2, K3, K5, K54, and K57 strains in mastitis milk samples, with close evolutionary relationships to human hypervirulent strains, suggesting a potential risk to public health.
MICROBES AND INFECTION
(2023)
Article
Agriculture, Dairy & Animal Science
S. McDougall, J. Penry, D. Dymock
Summary: The study indicated that bacterial isolates from organic herds had lower antibiotic resistance compared to herds using dry cow therapy (DCT). However, the differences in MIC distributions occurred below clinical breakpoints, suggesting that bacteriological cure rates may not be affected by isolates with different MIC levels.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Bruno Stefanon, Michela Cintio, Sandy Sgorlon, Elisa Scarsella, Danilo Licastro, Alfonso Zecconi, Monica Colitti
Summary: This study investigated the microRNA (miRNA) cargos of exosomes isolated from milk in relation to the healthy conditions of the mammary gland. Differential expression analysis identified differentially expressed miRNAs in the comparisons between healthy cows, cows at risk of mastitis, and cows with subclinical mastitis. The analysis of miRNA cargos in milk exosomes can be considered as a promising approach to study the complex molecular machinery set in motion in response to mastitis in dairy cows.
Article
Agriculture, Dairy & Animal Science
Wenpeng Zhao, Yuxiang Shi, Gang Liu, Jingyue Yang, Bing Yi, Yongxia Liu, John P. Kastelic, Bo Han, Jian Gao
Summary: The bacteriophage CM8-1 isolated from dairy farm wastewater showed promising results in treating murine K. pneumoniae mastitis by reducing bacterial counts, improving mammary gland tissue morphology, and decreasing expression of pro-inflammatory factors. The phage had stable biological characteristics and effectively suppressed K. pneumoniae mastitis when administered into the mammary gland in mice 2 hours after bacterial inoculation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hongyuan Jing, Yu Chen, Changwei Qiu, Meng-yao Guo
Summary: This study characterized the lncRNA profiles in mammary epithelial cells of dairy cows using RNA-sequencing technology, and identified 14 specific lncRNAs related to selenium and their predicted target genes. Functional annotation was used to elucidate the biological function and potential pathways involved. The findings provide novel insights for exploring molecular markers for protecting against mastitis in dairy cows.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Veterinary Sciences
Takeshi Tsuka, Hiroichi Ozaki, Daisuke Saito, Toshiyuki Murase, Yoshiharu Okamoto, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Yusuke Murahata, Tomohiro Imagawa
Summary: CTX-M-2-producing Klebsiella oxytoca strains with high ESBL production rates may spread clonally within a farm, while the bla(CTX-M-2) gene in Klebsiella pneumoniae may disseminate among farms through different plasmids. Monitoring ESBL genes, including bla(CTX-M-2), among causative agents of bacterial mastitis in cows can help develop relevant treatments and control practices.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Anri Timonen, Marju Sammul, Suvi Taponen, Tanel Kaart, Kerli Motus, Piret Kalmus
Summary: This study investigated the usage of antimicrobial drugs in cows with clinical mastitis in large dairy herds in Estonia. The findings revealed that different treatment methods and drugs could impact the outcome of the treatment, and further research is needed to understand the factors that influence veterinarians' choices.
Article
Agriculture, Dairy & Animal Science
Mengling Wang, Meng Cai, Xiaoyan Zhu, Xuemei Nan, Benhai Xiong, Liang Yang
Summary: Extracellular vesicles in milk play important roles in mastitis development. This study analyzed milk-derived extracellular vesicles from cows with mastitis and healthy cows using proteomic analysis. Results showed that mammary inflammation alters milk-derived extracellular vesicle protein cargos, particularly antimicrobial peptides. These findings provide valuable insights into the functions and pathways of milk-derived extracellular vesicle proteins.