Article
Biotechnology & Applied Microbiology
Deborah Hoyle, Marianne Keith, Helen Williamson, Kareen Macleod, Heather Mathie, Ian Handel, Carol Currie, Anne Holmes, Lesley Allison, Rebecca McLean, Rebecca Callaby, Thibaud Porphyre, Sue C. Tongue, Madeleine K. Henry, Judith Evans, George J. Gunn, David L. Gally, Nuno Silva, Margo E. Chase-Topping
Summary: Cattle in Scotland have a high prevalence of non-O157 serogroups O103 and O26 as well as high levels of Shiga toxin presence. Multiple serogroups are detected in herds and exhibit regional and seasonal effects on prevalence. This study emphasizes the potential risk to public health from non-O157 STEC in Scottish cattle.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Zahie Abboud, Lucia Galuppo, Marco Tolone, Maria Vitale, Roberto Puleio, Marwan Osman, Guido Ruggero Loria, Monzer Hamze
Summary: This study investigated the etiology of mastitis-causing pathogens in Northern Lebanon, identifying Streptococcus uberis, Streptococcus agalactiae, E. coli, and Staphylococcus aureus as the most frequently identified species. The study also found resistance to common antibiotics among bacteria and detected ESBL and AmpC genes in some E. coli isolates. These findings highlight the alarming risk of antimicrobial resistance in the Lebanese dairy chain.
Article
Microbiology
Heather Glassman, Christina Ferrato, Linda Chui
Summary: Non-O157 serogroups of Shiga toxin-producing Escherichia coli (STEC) play a significant role in disease burden, but are often overlooked by traditional detection methods. This study investigated the epidemiology of non-O157 STEC in Alberta, Canada from 2018 to 2021. The results showed increased detection during the summer months, with the most common serogroups being O26, O103, O111, O121, O118, and O71. Further characterization of their virulence factors and clinical impact is necessary.
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Michal Zolkiewski
Summary: ClpB, a molecular chaperone in pathogenic bacteria, plays a crucial role in their survival and infection under host-induced stress, making it a promising target for novel antimicrobial therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Multidisciplinary Sciences
Sonika Sonika, Samer Singh, Saurabh Mishra, Shashikala Verma
Summary: Toxin-Antitoxin (TA) systems are widely found in prokaryotes and play crucial roles in various biological processes, including plasmid maintenance, phage inhibition, stress response, biofilm formation, and dormant persister cell generation. These systems are particularly abundant in pathogenic intracellular micro-organisms and contribute to their adaptation to the harsh host environment, such as nutrient deprivation, oxidation, immune response, and antimicrobials. Despite some controversies, TA loci have been shown to be involved in successful infection, intracellular survival, colonization, and chronic infection, highlighting their importance in bacterial virulence and pathogenesis.
Article
Microbiology
Manuel G. Ballesteros-Monrreal, Margarita M. P. Arenas-Hernandez, Edwin Barrios-Villa, Josue Juarez, Maritza Lizeth alvarez-Ainza, Pablo Taboada, Rafael de la Rosa-lopez, Enrique Bolado-Martinez, Dora Valencia
Summary: Urinary tract infections caused by Uropathogenic Escherichia coli in women in Sonora, Mexico show diverse bacterial morphotypes, virulence genes, and phenotypes, with high resistance to various antibiotics. The presence of morphotypes in urine sediment is crucial in urine analysis, and the clinical isolates of UPEC in this study have the potential to cause different types of UTIs.
Article
Microbiology
Fengwei Jiang, Yan Yang, Zhao Mao, Wentong Cai, Ganwu Li
Summary: The study showed that microaerobic conditions enhance the expression of virulence factors in a porcine Shiga toxin-producing ETEC strain and investigated the role of the aerobic respiration control regulator ArcA in pathogenicity and virulence regulation in STEC/ETEC. Deletion of arcA in a mouse model caused less severe pathology, and the arcA mutant displayed lower colonization levels and weight gain. ArcA positively regulated the expression of key virulence factors and competed with H-NS to counteract repression. Overall, ArcA plays a key role in the pathogenicity and virulence expression of porcine STEC/ETEC.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Frederic Auvray, Clemence Bieche-Terrier, Maryse Michele Um, Veronique Dupouy, Nathalie Nzuzi, Laure David, Laurent Allais, Marie Drouet, Eric Oswald, Delphine Bibbal, Hubert Brugere
Summary: Analysis of fecal samples from veal calves slaughtered in France showed that O103:H2 and O26:H11 accounted for 73% of the seven serotypes of Shiga toxin-producing Escherichia coli (STEC), followed by O145:H28 and O157:H7. There were two peaks of higher prevalence during spring and fall, indicating a seasonal variation in STEC carriage. It is necessary to implement interventions in veal calf farming to reduce the risk of STEC contamination in meat production and the food chain.
VETERINARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Emile Auria, Lise Hunault, Patrick England, Marc Monot, Juliana Pipoli Da Fonseca, Mariette Matondo, Magalie Duchateau, Yannick D. N. Tremblay, Bruno Dupuy
Summary: The ability of Clostridioides difficile to form biofilms during infection and its association with recurrence and persistence are discussed in this study. The researchers investigate the role of the lipoprotein CD1687 in biofilm formation induced by deoxycholate (DCA). They found that CD1687 is necessary for biofilm formation, and its overexpression alone can induce biofilm formation. Furthermore, CD1687 affects the expression of transporters and metabolic pathways and binds DNA in a non-specific manner, suggesting its involvement in mediating the interaction between cells and the biofilm matrix.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Immunology
James R. Johnson, Connie Clabots, Stephen B. Porter, Tricia Bender, Brian D. Johnston, Paul Thuras
Summary: This study found that ST131-H30 subclone of Escherichia coli exhibits exceptional intestinal persistence, possibly due to a combination of fluoroquinolone resistance and virulence factors. These findings provide new insights for further study on the pandemic emergence of ST131-H30.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Mathew Stracy, Olga Snitser, Idan Yelin, Yara Amer, Miriam Parizade, Rachel Katz, Galit Rimler, Tamar Wolf, Esma Herzel, Gideon Koren, Jacob Kuint, Betsy Foxman, Gabriel Chodick, Varda Shalev, Roy Kishony
Summary: Currently, the treatment of bacterial infections focuses on choosing antibiotics based on the susceptibility of the pathogen, but there is less attention given to the risk of treatment-induced resistance. By using genomic sequencing and machine learning analysis, it is possible to predict and minimize the emergence of resistance at the individual-patient level.
Article
Ecology
Ayush Pathak, Daniel C. Angst, Ricardo Leon-Sampedro, Alex R. Hall
Summary: Some bacterial resistance mechanisms can degrade antibiotics, potentially protecting neighboring susceptible cells. However, the effects of such mechanisms on bacterial communities of more than two species are not well understood. By conducting experiments on multispecies communities, we found that resistance in one species reduced antibiotic inhibition of other species, but the extent of benefit varied among species.
Article
Infectious Diseases
Momna Rubab, Deog-Hwan Oh
Summary: The study investigated the antibiotic resistance of Shiga toxin-producing Escherichia coli (STEC) isolates, finding that most strains exhibited phenotypic resistance, with common resistant genes including ere(A), aadA1, and aac(3)-I. These results underscore the importance of monitoring multidrug resistance globally.
Article
Microbiology
Dalmasso Guillaume, Beyrouthy Racha, Brugiroux Sandrine, Ruppe Etienne, Guillouard Laurent, Bonnin Virginie, Saint-Sardos Pierre, Ghozlane Amine, Gaumet Vincent, Barnich Nicolas, Delmas Julien, Bonnet Richard
Summary: Without the use of antibiotics, the plasmid-mediated resistance gene mcr-1 enhances the colonization ability of Escherichia coli in the gut, but impairs its lethal effect. This improvement in gut fitness is associated with a downregulation of intestinal inflammatory markers and the preservation of intestinal microbiota composition.
Article
Chemistry, Analytical
Sung-Yoon Ahn, Mira Kim, Ji-Eun Bae, Iel-Soo Bang, Sang-Woong Lee
Summary: The toxicity of bacteria in indoor air is important to consider, and deep learning technology can help predict it.
