期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 70, 期 3, 页码 265-271出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.70.265
关键词
chemoreceptor; hypercapnic acidosis; inwardly rectifying K+ channel; medulla oblongata
The inwardly rectifying K+ channels, Kir1.1, Kir2.3 and Kirz4.1-Kir5.1, are the candidate chemosensory molecules for CO2/ H+. We determined the mRNA expression and immunohistochemical localization of these channels in the medulla oblongata. of the rat. RT-PCR analysis revealed mRNAs of Kir1.1, Kir2.3, Kir4.1 and Kir5.1 were detected in the medulla. The immunoreactivities for Kir1.1, Kir2.3, Kir4. 1, and Kir5.1 were observed in the medulla, and immunolabeling pattern was varied by the subunit. Immunoreactivities for Kir1.1 and Kir2.3 were observed in the nerve cell bodies and glial cells both in the chemosensory areas [nucleus tractus solitarius (NTS), nucleus raphe obscurus (RO), pre-Botzinger complex (PreBotC)] and non-chemosensory area [hypoglossal nucleus (XII), inferior olive nucleus (10)]. Kir4.1 immunoreactivity was observed in the glial cells and neuropil, especially in XII and IO. Kir5.1 immunoreactivity was observed in the nerve cell bodies in the XII, RO, and PreBotC, but not in the NTS or IO. In the NTS, a dense network of varicose nerve fibers showed immunoreactivity for Kir5.1. Our findings suggest that Kir channels may not act specific to the central chemoreception, but regulate the ionic properties of cellular membranes in various neurons and glial cells.
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