期刊
PROCESS BIOCHEMISTRY
卷 50, 期 12, 页码 2168-2176出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2015.10.014
关键词
Large-ring cyclodextrin; Amylomaltase; Tapioca starch; Vitamin E acetate; Solubility enhancement
资金
- NRCT-NSTDA Strategic Research Funding under Cassava Project [P-12-00733, P-13-00233]
- IIAC of the Chulalongkorn University Centenary Academic Development Project
- Thailand Research Fund [IRG 5780008]
Recombinant amylomaltase from. Corynebacterium glutamicum (CgAM) was used to synthesize large-ring cyclodextrins (LR-CDs) from tapioca starch. A mixture of LR-CDs (CD22-CD54) was obtained from both raw and soluble tapioca starch, but with different ratio of main products (CD25-CD30). Almost threefold higher amount of products was formed indicating the preference for raw tapioca starch. The optimal condition was to incubate 1.0% (w/v) starch with 0.05 U/ml CgAM in buffer pH 6.0 at 30 degrees C for 18 h. Total LR-CDs yield was 60.5 mg/g starch. LR-CDs were resistant to hydrolysis by Rhizopus sp. glucoamylase but completely hydrolyzed by CgAM at high enzyme concentration. Solid inclusion complex between LR-CDs mixture and vitamin E acetate was formed, as analyzed by FTIR. For complex preparation, the 1:10 mol ratio of LR-CDs to vitamin E was chosen because maximum amount of the vitamin was bound to LR-CDs and high complex yield was obtained. Aqueous solubility of vitamin E acetate in complex form increased 800-fold while the antioxidant activity decreased about 30%. This is the first work to demonstrate the use of tapioca starch in LR-CDs synthesis by CgAM and the complex formation between LR-CDs and vitamin E acetate as solubility enhancement process of vitamin. (C) 2015 Elsevier Ltd. All rights reserved.
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