期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 23, 期 1, 页码 108-111出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/104063871102300118
关键词
Coxiella burnetii; Q fever; real-time polymerase chain reaction; ring-trial
资金
- non-statutory zoonoses surveillance budget [FZ2100]
- VLA
- MedVetNet Workpackage [25]
The bacterium Coxiella burnetii, which has a wide host range, causes Q fever. Infection with C burnetii can cause abortions, stillbirth, and the delivery of weak offspring in ruminants. Coxiella burnetii infection is zoonotic, and in human beings it can cause chronic, potentially fatal disease. Real-time polymerase chain reaction (PCR) is increasingly being used to detect the organism and to aid in diagnosis both in human and animal cases. Many different real-time PCR methods, which target different genes, have been described. To assess the comparability of the C. burnetii real-time PCR assays in use in different European laboratories, a panel of nucleic acid extracts was dispatched to 7 separate testing centers. The testing centers included laboratories from both human and animal health agencies. Each laboratory tested the samples using their in-house real-time PCR methods. The results of this comparison show that the most common target gene for real-time PCR assays is the IS1111 repeat element that is present in multiple copies in the C. burnetii genome. Many laboratories also use additional real-time PCR tests that target single-copy genes. The results of the current study demonstrate that the assays in use in the different laboratories are comparable, with general agreement of results for the panel of samples.
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