Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Frederik Broszeit, Rosanne J. van Beek, Luca Unione, Theo M. Bestebroer, Digantkumar Chapla, Jeong-Yeh Yang, Kelley W. Moremen, Sander Herfst, Ron A. M. Fouchier, Robert P. de Vries, Geert-Jan Boons
Summary: Recent studies have shown that A/H3N2 viruses have evolved binding specificity to certain receptors, leading to inability to agglutinate erythrocytes. Glycan remodeling approach enables installation of functional receptors on erythrocytes, facilitating vaccine design.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Fan Yang, Yixin Xiao, Fumin Liu, Hangping Yao, Nanping Wu, Haibo Wu
Summary: A rapid detection method for H7N9 avian influenza virus (AIV) was established using monoclonal antibodies against hemagglutinin (HA) to develop an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA). AC-ELISA showed high specificity and sensitivity, with a detection limit of 3.9 ng/mL for H7N9 HA protein and 2(-2)HA unit/100 mu L for live H7N9 AIV. The technique proved to be rapid and practical for H7N9 AIV detection, with a sensitivity of 94.96% and specificity of 88.24% compared to conventional virus isolation detection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Microbiology
Maria Alessandra De Marco, Claudia Cotti, Elisabetta Raffini, Matteo Frasnelli, Alice Prosperi, Irene Zanni, Chiara Romanini, Maria Rita Castrucci, Chiara Chiapponi, Mauro Delogu
Summary: In this study, it was found that influenza A viruses are circulating in wild boar populations in Northern Italy, suggesting the occurrence of spillover events from domestic pigs to wild boars.
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Immunology
Jieshi Yu, Chithra Sreenivasan, Zhizhang Sheng, Shao-Lun Zhai, Jared W. Wollman, Sisi Luo, Chen Huang, Rongyuan Gao, Zhao Wang, Radhey S. Kaushik, Jane Christopher-Hennings, Eric Nelson, Ben M. Hause, Feng Li, Dan Wang
Summary: This study developed a swine influenza vaccine based on consensus H3, which can generate a broad antibody response against different circulating subtypes. Pigs vaccinated with this vaccine were fully protected against H3N2 viruses and further study of the consensus H3 vaccine platform is warranted for broad protection against diverse swine influenza viruses.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Letter
Immunology
Wanying Sun, Samuel S. M. Cheng, Kristy N. T. Lam, Tsz C. Kwan, Ricky W. K. Wong, Leo H. K. Lau, Gigi Y. Z. Liu, Leo L. H. Luk, John K. C. Li, Haogao Gu, Malik Peiris, Leo L. M. Poon
Summary: A study revealed the presence of a Eurasian avian-like H1N1 swine influenza virus with genes derived from the H9N2 subtype, indicating the reassortment between H9N2 viruses and swine influenza viruses in pigs in China.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Immunology
Annika Graaf, Philipp P. Petric, Julia Sehl-Ewert, Dinah Henritzi, Angele Breithaupt, Jacqueline King, Anne Pohlmann, Fabian Deutskens, Martin Beer, Martin Schwemmle, Timm Harder
Summary: Given the challenges of antigenic drift and interference with maternal antibodies in controlling swine influenza A virus infections, researchers evaluated the safety and efficacy of cold-passaged and reassortment techniques for vaccine development. The results showed that reassortant-incompetent chimeric bat-swIAV candidates demonstrated good safety profiles and induced protective immunity in mice and pigs.
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Microbiology
Zhenhong Zhou, Rui Zhu, Hongwei Yang, Longfa Xu, Hao Chen, Yuanyuan Wu, Zhichao Yin, Qiongzi Huang, Dongqing Zhang, Che Liu, Yuqiong Que, Jun Zhang, Ningshao Xia, Tong Cheng
Summary: Human Rhinoviruses are major pathogens causing respiratory tract diseases, particularly RV-C species which are associated with asthma. A rapid neutralization testing system for RV-C15 was developed in this study, which can detect infected cells and obtain neutralizing antibodies against RV-C15.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.