期刊
JOURNAL OF VASCULAR RESEARCH
卷 45, 期 3, 页码 251-258出版社
KARGER
DOI: 10.1159/000112940
关键词
endothelial nitric oxide synthase; aneurysm; aging; model
资金
- NHLBI NIH HHS [K08-HL079927, K08 HL079927, K08 HL079927-02] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [K08HL079927] Funding Source: NIH RePORTER
Background/Aims: Age-associated changes in endothelial nitric oxide synthase (eNOS) expression have not been definitively linked to the pathophysiology of aortic aneurysms. We examined the role of eNOS in human patients and an age-appropriate mouse model. Methods: eNOS transcripts and immunodetectable protein were assessed by quantitative PCR and immunohistochemistry in human ascending thoracic aneurysms (n = 29) and referent aortae (n = 31). Carotid aneurysms were induced with CaCl2 in young adult (3 months) and aged (18 months) C57BL/6 and eNOS-knockout (eNOS-KO) mice. Results: eNOS transcripts and protein were reduced in human aneurysms compared with controls, although aortic eNOS expression also decreased with patient age. Aged wild-type mice had significantly larger aneurysm diameter than young adult mice. Aged wild-type mice had reduced eNOS transcripts and protein compared with young adult mice. Aged eNOS-KO mice had smaller aneurysms compared with aged wild-type mice but similar size aneurysms compared with young eNOS-KO and young wild-type mice. Conclusion: eNOS expression is reduced in both aged human and aged mouse endothelium and eNOS expression is linked to aneurysm expansion in aged but not young adult mice. These findings support the relevance of age-associated changes in eNOS expression in clinical aneurysmal disease. Copyright (C) 2008 S. Karger AG, Basel.
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