Review
Biochemistry & Molecular Biology
Stephanie Sauve, Joseph Williamson, Adithya Polasa, Mahmoud Moradi
Summary: The major facilitator superfamily (MFS) of transporters consists of three classes of membrane transporters: symporters, uniporters, and antiporters. The conformational changes within the distinct transport cycles of MFS transporters are believed to follow the rocker-switch mechanism. This review compares the similarities and differences in conformational dynamics between symporters, uniporters, and antiporters of the MFS family using experimental and computational structural data.
Article
Microbiology
Ashley N. Williams, John Stavrinides
Summary: Pantoea Natural Product 3 (PNP-3) is an effective antibiotic against multi-drug resistant bacteria. The production and resistance of PNP-3 are regulated by two transporters, pnp3a and pnp3c. Disruption of pnp3a leads to impaired production and resistance, while disruption of pnp3c only reduces production. These findings provide important insights into the synthesis and resistance mechanism of PNP-3.
RESEARCH IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Jian Diao, Shuxuan Li, Ling Ma, Ping Zhang, Jianyang Bai, Jiaqi Wang, Xiaoqian Ma, Wei Ma
Summary: This study systematically analyzed the structure and function of genes and proteins in the PtrMFS family, indicating that poplar MFS genes play key roles in plant development and response to biological stressors.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Manuel F. Varela, Jerusha Stephen, Deeksha Bharti, Manjusha Lekshmi, Sanath Kumar
Summary: Bacterial pathogens resistant to multiple antimicrobial agents through active efflux pose a serious threat to public health. This review discusses recent molecular studies focused on modulating the antimicrobial efflux transporters to restore the clinical efficacy of infectious disease chemotherapy.
Article
Biochemistry & Molecular Biology
Ki-Hwa Kim, Chang Woo Lee, Bashu Dev Pardhe, Jisub Hwang, Hackwon Do, Yung Mi Lee, Jun Hyuck Lee, Tae-Jin Oh
Summary: 7 alpha-Hydroxysteroid dehydrogenase (7 alpha-HSDH) plays a role in catalyzing the dehydrogenation of steroid substrates at the 7 alpha position. Structural studies reveal significant conformational changes in the apo form of 7 alpha-HSDH, with only the ternary complex structure showing a fully closed conformation. The transition from open to closed conformation is essential for catalytic activity, positioning important residues for catalysis.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Infectious Diseases
Jerusha Stephen, Fathima Salam, Manjusha Lekshmi, Sanath H. Kumar, Manuel F. Varela
Summary: This review article discusses the S. aureus-specific MFS multidrug efflux pump systems from a molecular mechanistic perspective, including structure-function relationships, modulation of antimicrobial resistance mediated by MFS drug efflux pumps, and direction for future investigation.
Article
Medicine, Research & Experimental
Angelika Janaszkiewicz, Agota Toth, Quentin Faucher, Helene Arnion, Nicolas Vedrenne, Chantal Barin-Le Guellec, Pierre Marquet, Florent Di Meo
Summary: The Organic Anion Transporter 1 (hOAT1) is a membrane transporter involved in drug elimination by the kidney. This study aimed to investigate fragments of hOAT1 structure and function in order to better understand its transport cycle. Molecular dynamics simulations suggested two potential binding sites for the substrate adefovir, including a new inner binding cavity. The binding modes of the co-substrate alpha-ketoglutarate were also explored. The findings provide insights into the transport impairments caused by hOAT1 mutations.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Diego del Alamo, Jens Meiler, Hassane S. Mchaourab
Summary: Transporters belonging to the LeuT-fold class mediate the import and exchange of hydrophilic and charged compounds. Despite extensive research on LeuT, there is still a lack of detailed understanding of the transport mechanisms for this class of membrane proteins.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Applied
Cong-Huan Wang, Zhan-Chen Gao, Weipeng Sun, Xugang Guo, Fei-Bao Zhang
Summary: BBFP and BBTP were synthesized by reacting dilithio(benzothienylbenzofuran) or dilithio(benzothienylbenzothiophene) with Diphenylphosphinic chloride, followed by reduction with trichlomsilane to afford the target products. Optical and electrochemical measurements, along with DFT calculations, showed that P⋯O noncovalent conformational locks played an important role in constructing highly planar BBFP, while BBTP exhibited a distorted structure with very low PL quantum yields.
Article
Microbiology
Jennifer C. Chang, Reid Wilkening, Kate M. Rahbari, Michael J. Federle
Summary: Cell-cell signaling mediated by Rgg-family transcription factors and their cognate pheromones plays a crucial role in regulating various phenotypes in Firmicutes. In particular, the Rgg2/3 quorum sensing system in Streptococcus pyogenes has been shown to influence the expression of secreted virulence factors and antibiotic susceptibility, with dysregulation by the deletion of an Rgg2/3 QS-regulated MFS transporter. This study highlights the complex regulatory mechanisms involved in the expression of virulence factors and antibiotic sensitivity in bacterial pathogens.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Chemistry, Physical
S. Kanthal, P. Manna, Md A. Ahmed, A. Banerjee, S. Adhikary, S. Kanungo, A. K. Yadav, S. N. Jha, D. Bhattacharyya, S. Bandyopadhyay
Summary: The study demonstrated that doping rare earth (RE)-Nd based cuprate francisite with cobalt significantly enhances magnetic properties, including magnetic hysteresis and magnetic field induced metamagnetic transition. First principle theoretical calculations and local structural studies help explain these phenomena and detect unexpected trends in magnetism with increasing cobalt doping.
APPLIED SURFACE SCIENCE
(2021)
Article
Microbiology
Wenzhe Tian, Jiayang Qin, Congcong Lian, Qingshou Yao, Xiuwen Wang
Summary: This study identified a mutant gene mfs coding for the major facilitator superfamily (MFS) protein in Bacillus coagulans Na-2, which has better resistance to sodium lactate stress. Expression of MFS-2-6 and MFS-Na-2 improved L-LA production at low pH, with MFS-Na-2 showing better performance likely due to more appropriate intracellular pH during fermentation. The MFS protein identified here has potential application in environmentally friendly L-LA production.
Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Medicinal
Karen M. Corbett, Leigh Ford, Dallas B. Warren, Colin W. Pouton, David K. Chalmers
Summary: Cyclosporins are a class of peptides with wide pharmaceutical applications, some of which exhibit high membrane permeability not easily explained by structural metrics. Experimental studies have been conducted to understand the structure and permeability of cyclosporins, including their interaction with metal complexes. Models have been proposed to explain the conformation-dependent permeability of cyclosporins and similar compounds, with gaps in current knowledge identified for further exploration.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Burak Guzelturk, Thomas Winkler, Tim W. J. Van de Goor, Matthew D. Smith, Sean A. Bourelle, Sascha Feldmann, Mariano Trigo, Samuel W. Teitelbaum, Hans-Georg Steinruck, Gilberto A. de la Pena, Roberto Alonso-Mori, Diling Zhu, Takahiro Sato, Hemamala Karunadasa, Michael F. Toney, Felix Deschler, Aaron M. Lindenberg
Summary: Diffuse X-ray scattering with femtosecond resolution reveals the formation and relaxation of polaronic distortions in halide perovskites. These structural changes are quantified and correlated to transient changes in carrier effective mass.
Review
Water Resources
Jyoti Chawla, Divya Singh, Baranidharan Sundaram, Arun Kumar
EXPOSURE AND HEALTH
(2018)
Article
Water Resources
Shushobhit Chaudhary, C. T. Dhanya, Arun Kumar
HYDROLOGY RESEARCH
(2018)
Article
Chemistry, Multidisciplinary
Nathan C. Frey, Hemant Kumar, Babak Anasori, Yury Gogotsi, Vivek B. Shenoy
Article
Chemistry, Physical
Hooman Yaghoobnejad Asl, Jintao Fu, Hemant Kumar, Samuel S. Welborn, Vivek B. Shenoy, Eric Detsi
CHEMISTRY OF MATERIALS
(2018)
Article
Engineering, Multidisciplinary
Arun Kumar, Kaushal Kumar, P. K. Ghosh, Ankit Rathi, K. L. Yadav, Raman
COMPOSITES PART B-ENGINEERING
(2018)
Article
Materials Science, Multidisciplinary
Pooja Kapoor, Arun Kumar, Munish Sharma, Jagdish Kumar, Ashok Kumar, P. K. Ahluwalia
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
(2018)
Article
Chemistry, Multidisciplinary
Dequan Er, Han Ye, Nathan C. Frey, Hemant Kumar, Jun Lou, Vivek B. Shenoy
Article
Multidisciplinary Sciences
Hemant Kumar, Janet S. Finer-Moore, Xiaoxu Jiang, Irina Smirnova, Vladimir Kasho, Els Pardon, Jan Steyaert, H. Ronald Kaback, Robert M. Stroud
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Chemistry, Multidisciplinary
Nathan C. Frey, Arkamita Bandyopadhyay, Hemant Kumar, Babak Anasori, Yury Gogotsi, Vivek B. Shenoy
Article
Optics
Qudsia Rubani, Sindhu Hak Gupta, Shuchismita Pani, Arun Kumar
Article
Energy & Fuels
Manish Kumar, Arun Kumar
IEEE JOURNAL OF PHOTOVOLTAICS
(2019)
Article
Multidisciplinary Sciences
Hemant Kumar, Janet Finer-Moore, Irina Smirnova, Vladimir Kasho, Els Pardon, Jan Steyaert, H. Ronald Kaback, Robert M. Stroud
Article
Biochemistry & Molecular Biology
Nancy Tripathi, Bharat Goel, Nivedita Bhardwaj, Bharat Sahu, Hemant Kumar, Shreyans K. Jain
Summary: This study identified potential inhibitors of 2019-nCoV protease using ligand- and structure-based approaches. Through screening and calculations, two compounds with good activity and desirable properties were identified, which could serve as drug candidates against 2019-nCoV.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Ecology
Divya Singh, Arun Kumar