Review
Biochemistry & Molecular Biology
Rebecca J. J. Taylor, Grigory Tagiltsev, John A. G. Briggs
Summary: COPI-coated vesicles are involved in transport between Golgi stacks and retrograde transport from the Golgi to the endoplasmic reticulum. This process is regulated by small GTPases of the Arf family, which recruit the COPI coat to the membrane for vesicle formation. Understanding the interplay between coatomer, Arf GTPases, and their effectors is essential to comprehend the mechanism of COPI-coated vesicle trafficking.
Article
Genetics & Heredity
William L. Macken, Annie Godwin, Gabrielle Wheway, Karen Stals, Liliya Nazlamova, Sian Ellard, Ahmed Alfares, Taghrid Aloraini, Lamia AlSubaie, Majid Alfadhel, Sulaiman Alajaji, Htoo A. Wai, Jay Self, Andrew G. L. Douglas, Alexander P. Kao, Matthew Guille, Diana Baralle
Summary: This study identified a novel recessive coatopathy characterized by severe developmental delay, cataracts, and variable microcephaly. Genetic analysis revealed variants in the COPB1 gene in two families, and CRISPR/Cas9 modelling in Xenopus tropicalis replicated the human syndrome features. This research highlights the importance of COPI subunits in brain development and human health, demonstrating the utility of exome and genome sequencing combined with Xenopus tropicalis CRISPR/Cas modelling for identifying and characterizing rare disease genes.
Article
Biology
Swapneeta S. Date, Peng Xu, Nathaniel L. Hepowit, Nicholas S. Diab, Jordan Best, Boyang Xie, Jiale Du, Eric R. Strieter, Lauren P. Jackson, Jason A. MacGurn, Todd R. Graham
Summary: This study reveals the role of COPI in SNARE trafficking and Golgi SNARE localization through ubiquitin recognition, and demonstrates how ubiquitination enhances the binding between Arf and COPI with Gos1.
Article
Biology
Ludovic Enkler, Bruno Rinaldi, Johan Owen de Craene, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D. Becker
Summary: COPI-coated vesicles are involved in Golgi-to-ER and intra-Golgi trafficking pathways, mediating retrieval of ER resident proteins. In mammalian cells, proteins like GORAB, SCYL1, and SCYL3 regulate Golgi morphology and protein glycosylation in conjunction with the COPI machinery. The yeast protein Cex1, homologous to mammalian SCYL proteins, is a component of the COPI machinery and can potentially be used to study neurodegenerative disorders.
Article
Biochemistry & Molecular Biology
Judit Sanchez-Simarro, Cesar Bernat-Silvestre, Fernando Aniento, Maria Jesus Marcote
Summary: COPI complex formed by seven subunits is recruited to Golgi membranes to shuttle vesicles between Golgi apparatus and ER. Mutants of Arabidopsis beta-COP genes show increased sensitivity to chloride ions when exposed to Na+ or Cl- salts.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Plant Sciences
Judit Sanchez-Simarro, Fernando Aniento, Maria Jesus Marcote
Summary: Coat protein I (COPI) and Coat protein II (COPII) coated vesicles play a role in protein transport during early secretory pathway. The role of COPI components in gametogenesis has not been thoroughly studied, unlike the known essential role of COPII vesicles' components. In Arabidopsis, COPI is composed of a heptameric complex with multiple isoforms, and this study found that two isoforms of the beta'-COP subunit are necessary for both female and male gametophyte development. Reciprocal crosses showed that gametophytes lacking both beta'1-COP and beta'2-COP cannot be transmitted.
PLANT REPRODUCTION
(2023)
Article
Cell Biology
Luis Wong-Dilworth, Carmen Rodilla-Ramirez, Eleanor Fox, Steffen D. Restel, Alexander Stockhammer, Petia Adarska, Francesca Bottanelli
Summary: By combining CRISPR-Cas9 gene editing with STED super-resolution microscopy, Wong-Dilworth et al. investigate the localization and roles of ADP-ribosylation factor (ARF) GTPases in cellular membranes. They find that ARF1, ARF4, and ARF5 exist on segregated nanodomains in the cis-Golgi and ER-Golgi intermediate compartments, indicating distinct roles in COPI recruitment. Additionally, ARF1 and ARF3 localize to segregated nanodomains on the trans-Golgi network and are present on TGN-derived post-Golgi tubules, suggesting distinct functions in post-Golgi sorting.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Virology
Natali Ozber, Paolo Margaria, Charles T. Anderson, Massimo Turina, Cristina Rosa
Summary: This study reveals the localization of OuMV MP to the trans-Golgi network (TGN) and the important roles of two putative sorting motifs in targeting MP to plasmodesmata (PD) and achieving efficient systemic infection. Additionally, the study identifies a series of host factors that interact with OuMV MP and are associated with vesicle trafficking and membrane lipids.
FRONTIERS IN VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Eugenia Butkevich, Kai Bodensiek, Nikta Fakhri, Kerstin von Roden, Iwan A. T. Schaap, Irina Majoul, Christoph F. Schmidt, Dieter R. Klopfenstein
NATURE COMMUNICATIONS
(2015)
Article
Chemistry, Physical
R. Schoenherr, M. Klinge, J. M. Rudolph, K. Fita, D. Rehders, F. Luebber, S. Schneegans, I. V. Majoul, M. Duszenko, C. Betzel, A. Brandariz-Nunez, J. Martinez-Costas, R. Duden, L. Redecke
STRUCTURAL DYNAMICS-US
(2015)
Article
Cell Biology
Lauren P. Jackson, Michael Lewis, Helen M. Kent, Melissa A. Edeling, Philip R. Evans, Rainer Duden, David J. Owen
DEVELOPMENTAL CELL
(2012)
Article
Biochemistry & Molecular Biology
Victoria J. Miller, Prateek Sharma, Tetyana A. Kudlyk, Laura Frost, Adam P. Rofe, Irene J. Watson, Rainer Duden, Martin Lowe, Vladimir V. Lupashin, Daniel Ungar
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Multidisciplinary Sciences
Irina V. Majoul, Liang Gao, Eric Betzig, Daria Onichtchouk, Eugenia Butkevich, Yuri Kozlov, Feliksas Bukauskas, Michael V. L. Bennett, Jennifer Lippincott-Schwartz, Rainer Duden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Cell Biology
Sebastian Springer, Per Malkus, Britta Borchert, Ursula Wellbrock, Rainer Duden, Randy Schekman
Article
Multidisciplinary Sciences
Rose Willett, Tetyana Kudlyk, Irina Pokrovskaya, Robert Schoenherr, Daniel Ungar, Rainer Duden, Vladimir Lupashin
NATURE COMMUNICATIONS
(2013)
Meeting Abstract
Chemistry, Multidisciplinary
L. Redecke, M. Klinge, R. Schoenherr, D. Rehders, D. Oberthuer, B. Sommer, J. Martinez-Costas, M. Duszenko, R. Duden, C. Betzel
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2014)
Article
Multidisciplinary Sciences
Sophie M. Travis, Bashkim Kokona, Robert Fairman, Frederick M. Hughson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Sophie M. Travis, Kevin DAmico, I-Mei Yu, Conor McMahon, Safraz Hamid, Gabriel Ramirez-Arellano, Philip D. Jeffrey, Frederick M. Hughson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Cell Biology
Sophie M. Travis, Brian P. Mahon, Sabine Petry
Summary: The microtubule cytoskeleton is crucial for intracellular organization and regulated motion. Branching microtubule nucleation is a key pathway for generating microtubules, and recent research has focused on characterizing the factors and regulation involved in this process, as well as its implications in human disease.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)