Article
Endocrinology & Metabolism
Dylan J. J. Ritter, Dharmendra Choudhary, Gokhan Unlu, Ela W. W. Knapik
Summary: This study reveals that Rgp1 plays a crucial role in craniofacial cartilage development and the secretion of collagen II. Loss of Rgp1 leads to altered movement of Rab6a(+) vesicular compartments and activation of an Arf4b-mediated stress response in chondrocytes, resulting in cell death.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ryan Y. Nguyen, Aidan T. Cabral, Alejandro Rossello-Martinez, Alessandro Zulli, Xiangyu Gong, Qiuting Zhang, Jing Yan, Michael Mak
Summary: This study develops a collagen hydrogel that induces the formation of heterogeneous mesoscopic architectures, known as collagen islands. These island-containing gels have highly tunable inclusions and mechanical properties, with regional enrichment in collagen concentration at the cell-scale. The collagen island architectures are used to investigate mesenchymal stem cell behavior and demonstrate alterations in cell migration and osteogenic differentiation. Moreover, induced pluripotent stem cells cultured in island-containing gels show sufficient architecture to induce mesodermal differentiation. Overall, this work highlights the importance of complex mesoscopic tissue architectures as bioactive cues in regulating cell behavior and presents a novel collagen-based hydrogel for tissue engineering applications.
ADVANCED MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Maria A. Cruz, Yamilet Gonzalez, Javier A. Velez A. Toro, Makan Karimzadeh, Anthony Rubbo, Lauren Morris, Ramapaada Medam, Taylor Splawn, Marilyn Archer, Russell J. Fernandes, James E. Dennis, Thomas J. Kean
Summary: This study successfully established a high-throughput platform for identifying optimal physiological culture parameters for chondrogenesis by using a Design of Experiments approach and secreted reporter cells in 3D aggregate culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Agustina Setiawati, Sungwoo Jeong, Albertus Ivan Brilian, Sang Ho Lee, Jin-Gon Shim, Kwang-Hwan Jung, Kwanwoo Shin
Summary: In this study, a hybrid extracellular matrix (ECM) scaffold was successfully fabricated, and it was demonstrated that the fibronectin (FN)-rich hybrid ECM scaffold promoted successful settling of liver cells. This method holds promise for the fabrication of scaffolds that provide tailored cellular microenvironment for specific organs and can serve as novel pathways for organ replacement or regeneration.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Dafydd O. Visscher, Hyeongjin Lee, Paul P. M. van Zuijlen, Marco N. Helder, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: The study developed a printable bioink cdECMMA derived from porcine auricular cartilage, which exhibited good rheological properties and mechanical integrity. Chondrocytes in the printed cdECMMA hydrogel maintained viability, proliferation capacity, and produced cartilage ECM components. The novel cell-based bioprinting using this cartilage-specific dECMMA bioink offers an alternative option for auricular cartilage reconstruction.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
Danni Gong, Fei Yu, Meng Zhou, Wei Dong, Dan Yan, Siyi Zhang, Yan Yan, Huijing Wang, Yao Tan, Ying Chen, Bei Feng, Wei Fu, Yao Fu, Yang Lu
Summary: dcECM hydrogels derived from porcine ears through enzymatic digestion show good biocompatibility for delivering chondrocytes and forming subcutaneous cartilage in vivo. The structural and gelation kinetics of the hydrogels vary with ECM concentrations, with 10 mg/ml hydrogels supporting adhesion and proliferation of chondrocytes in vitro. In vivo, 10 mg/ml dcECM hydrogel grafts exhibit similar qualities to native cartilage, indicating their potential for tissue engineering applications in ear cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Pharmacology & Pharmacy
Amina T. Mneimneh, Mohammed M. Mehanna
Summary: Collagen-based scaffolds play a significant role in promoting regeneration, repair, and recovery of spinal cord injuries by providing a suitable environment for tissue repair, axonal regeneration, and vascularization. They are biocompatible, biodegradable, and widely used in pharmaceutical, cosmetic, food industries, and tissue engineering. Functionalized with different ligands and factors, collagen in scaffolds enhances its binding specificity and activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Pharmacology & Pharmacy
Samantha Lo, Mh Busra Fauzi
Summary: Collagen, as a widely studied natural component, plays a major role in tissue repair and regeneration in tissue engineering. Nano collagen, a relatively new material involved in nanotechnology, has potential advantages over traditional collagen design due to its nano-size, making it suitable for various medical applications and cosmetic purposes.
Article
Biochemistry & Molecular Biology
Ghazi O. Bou Ghanem, Dmitry Koktysh, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Lauren K. Wareham, David J. Calkins
Summary: This study investigates the potential of collagen mimetic peptides (CMPs) in repairing damaged collagen in ocular tissues. The results demonstrate that CMPs can restore the stiffness and structure of collagen in optic nerve head tissues, offering a promising therapeutic avenue for ocular disorders involving collagen remodeling and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Maryam Khajavi, Abdolmajid Hajimoradloo, Mojgan Zandi, Mohamad Pezeshki-Modaress, Shahin Bonakdar, Abbas Zamani
Summary: The study successfully engineered a cartilage-like scaffold using ECM from sturgeon fish cartilage to stimulate cartilaginous matrix synthesis in human adipose stem cells. The porous scaffold derived from fish cartilage ECM showed excellent potential for cartilage tissue engineering, supporting cell adhesion, proliferation, and chondrogenic differentiation. The scaffold was demonstrated to facilitate the synthesis of specific cartilage proteins, such as collagen type II and aggrecan, making it a promising option for tissue regeneration.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Review
Cell & Tissue Engineering
Mahsa Nouri Barkestani, Sina Naserian, Georges Uzan, Sara Shamdani
Summary: Tissue engineering has gained attention as a new hope for treating degenerative disorders, involving complex processes of decellularization and recellularization. Additionally, focus is on improving post-decellularization methods to enhance efficiency.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Materials Science, Biomaterials
Theofanis Stampoultzis, Yanheng Guo, Naser Nasrollahzadeh, Peyman Karami, Dominique P. Pioletti
Summary: Articular cartilage is a mechanically sensitive tissue where chondrocytes perceive and react to physical cues. This study explores the role of temperature as a regulatory signal for chondrocyte function and evaluates the effects of thermal and/or mechanical stimulation on chondrocytes in different types of scaffolds. The results suggest that the combination of dynamic thermal and mechanical stimuli has superior effects on chondrogenic genes and proteoglycan accumulation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Polymer Science
Jing Zhou, Nier Wu, Jinshi Zeng, Ziyu Liang, Zuoliang Qi, Haiyue Jiang, Haifeng Chen, Xia Liu
Summary: This study developed a hydrogel scaffold for cartilage tissue engineering using a secondary crosslinking method. The hydrogel exhibited excellent pore structure and water absorption ability, and was non-toxic to cells. The addition of decellularized extracellular matrix and a multi-channeled structure promoted chondrogenic differentiation of adipose-derived stromal cells and engineered cartilage formation. This hydrogel scaffold shows great potential for cartilage tissue engineering.
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)