Article
Multidisciplinary Sciences
Muge Ozkan, Yusuf Cem Eskiocak, Gerhard Wingender
Summary: The identification of immune effector functions, particularly those involving IL-10 production, is crucial for understanding immune responses. While IL-10(GFP) reporter lines can accurately report on IL-10 production by lymphoid cells, they may not be suitable for analyzing myeloid cell IL-10 production, as autofluorescence can interfere with GFP signals. This highlights a potential technical limitation when using GFP-reporter lines for studying myeloid cells in inflammation.
Article
Biochemical Research Methods
Jacob Beal, Brian Teague, John T. Sexton, Sebastian Castillo-Hair, Nicholas A. DeLateur, Meher Samineni, Jeffrey J. Tabor, Ron Weiss
Summary: Reliable and predictable engineering of cellular behavior is a key goal of synthetic biology. This study provides a quantitative mathematical bound on the relationship between predictability of results, model accuracy, measurement precision, and device characteristics. The findings can guide the measurement precision requirements for engineering genetic regulatory networks and recommend best practices for quality control of flow cytometry data.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Qian Chen, Mehdi Benamar, Tsz Man Fion Chan, Muyun Wang, Talal A. Chatila
Summary: Regulatory T (Treg) cells are a specialized subpopulation of CD4(+) T cells that maintain peripheral immune tolerance by suppressing excessive immune responses. The master regulator Foxp3 plays a crucial role in the development and function of Treg cells. Under inflammatory conditions, Treg cells may acquire characteristics of effector T cells, demonstrating their plasticity. During microbial infections, Treg cells act to limit immunopathology but may also pose a risk of pathogen persistence.
Article
Cell Biology
Nicola Beucke, Svenja Wingerter, Karin Haehnel, Lisbeth Aagaard Larsen, Kaare Christensen, Graham Pawelec, Kilian Wistuba-Hamprecht
Summary: The study found that latent cytomegalovirus infection affects the differentiation status of alpha beta and gamma delta T cells in humans. Additionally, genetic factors also influence the differentiation of gamma delta and alpha beta T cell subsets.
Article
Biochemistry & Molecular Biology
Valeria Orru, Francesca Virdis, Michele Marongiu, Valentina Serra, David Schlessinger, Marcella Devoto, Francesco Cucca, Edoardo Fiorillo
Summary: Extracellular vesicles (EVs) mediate cell interactions in biological processes. This study evaluated blood levels of EVs and platelet traits and conducted the first genome-wide association study (GWAS) on these traits. The results showed that EV levels decrease with age and are influenced by genetic factors. Several significant genetic signals were identified, suggesting that EV formation is not solely dependent on metabolic regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Ruihua Cao, Qiqi Wan, Xiangqian Xu, Shiqi Tian, Gehui Wu, Jingyi Wang, Tinglin Huang, Gang Wen
Summary: A new method was established in this study to differentiate DNA damage and membrane damage of fungal spores, elucidating the disinfection mechanism during chemical disinfection. The repeatability and applicability of this method were confirmed, and it was further applied to explore the inactivation mechanism during chlorine-based disinfection.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Immunology
Davide Moi, Bijun Zeng, Simone A. Minnie, Rituparna Bhatt, Jack Wood, David P. Sester, Roberta Mazzieri, Riccardo Dolcetti
Summary: Suitable methods to assess in vivo immunogenicity and therapeutic efficacy of cancer vaccines in preclinical cancer models are critical to overcome current limitations of cancer vaccines and enhance the clinical applicability of this promising immunotherapeutic strategy. Moreover, multiparametric assays to deeply characterize tumor-induced and therapy-induced immune modulation are relevant to design mechanism-based combination immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biochemistry & Molecular Biology
Alessandra Rossi, Ilenia Pacella, Silvia Piconese
Summary: T cells undergo activation and differentiation programs in a continuum of states that can be tracked using flow cytometry. The integration of signaling pathways with metabolic routes plays a crucial role in T cell function. Flow cytometry is a valuable tool for analyzing the integration of various features in T cells, with potential clinical applications in cancer immunotherapy monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Omar Perbellini, Chiara Cavallini, Roberto Chignola, Marilisa Galasso, Maria T. Scupoli
Summary: This study reveals a high variability in signaling networks across different T-ALL cell lines and provides insight into the characterization of these differences using phospho-specific flow cytometry.
Article
Medicine, Research & Experimental
Bartosz Skalski, Joanna Rywaniak, Jerzy Zuchowski, Anna Stochmal, Beata Olas
Summary: Studies have shown that phenolic compounds in sea buckthorn leaf and twig extracts have a protective effect on the cardiovascular system by reducing blood platelet activation. These extracts also have anticoagulant properties, making them potential natural supplements for anti-platelet and anticoagulant purposes.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Stephan Fuhrmann, Bernhard Reus, Oliver Frey, Alejandra Pera, Louis J. Picker, Florian Kern
Summary: The effect of CMV infection on non-CMV-specific T-cells is limited to a minority of individuals and is not closely related to the degree of memory subset differentiation of CMV-specific T-cells. Unknown predisposing factors might determine to what extent CMV infection affects non-CMV-specific T-cell differentiation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Mathangi Janakiraman, Shin-Young Na, Gurumoorthy Krishnamoorthy
Summary: A novel CD4 mutant allele was identified in this study, causing impaired development of CD4(+) helper T cells. The CRISPR mediated correction restored the normal expression of CD4, validating the significance of this mouse model for the study of CD4(+) T cells.
Review
Immunology
Ivon Johanna Rodriguez, Nicolas Lalinde Ruiz, Manuela Llano Leon, Laura Martinez Enriquez, Maria del Pilar Montilla Velasquez, Juan Pablo Ortiz Aguirre, Oscar Mauricio Rodriguez Bohorquez, Esteban Alejandro Velandia Vargas, Edgar Debray Hernandez, Carlos Alberto Parra Lopez
Summary: Aging leads to alterations in immune response, termed immunosenescence, characterized by decreased naive T cells and increased expression of CD57 and KLRG1 in memory T cells in older adults. Proinflammatory cytokines increase and telomere length decrease in old adult T cells. The heterogeneity and biases among studies hinder robust statistical analysis for identifying flow cytometry markers of T cell immunosenescence phenotype.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Allergy
Nami Shrestha Palikhe, Yingqi Wu, Emily Konrad, Vivek Dipak Gandhi, Brian H. Rowe, Harissios Vliagoftis, Lisa Cameron
Summary: This study found that during acute asthma exacerbations, the number of Th2 cells in peripheral blood correlated with ACQ6 and AQLQ scores, while ILC2 and eosinophil numbers did not. Subjects had higher CRTh2 expression during exacerbation compared to follow-up, and worse lung function. The decrease in CRTh2 expression during follow-up correlated with improvement in lung function.
Article
Public, Environmental & Occupational Health
Miyuki Hasegawa, Shamima Akter, Huanhuan Hu, Ikuko Kashino, Keisuke Kuwahara, Hiroko Okazaki, Naoko Sasaki, Takayuki Ogasawara, Masafumi Eguchi, Takeshi Kochi, Toshiaki Miyamoto, Tohru Nakagawa, Toru Honda, Shuichiro Yamamoto, Taizo Murakami, Makiko Shimizu, Akihiko Uehara, Makoto Yamamoto, Teppei Imai, Akiko Nishihara, Kentaro Tomita, Satsue Nagahama, Ai Hori, Maki Konishi, Isamu Kabe, Tetsuya Mizoue, Naoki Kunugita, Seitaro Dohi, T. Mizoue, S. Akter, H. Hu, Y. Inoue, A. Fukunaga, I Kashino, Z. Islam, M. Konishi, A. Nanri, K. Kurotani, K. Kuwahara, Y. Watanabe, T. Imai, T. Miyamoto, M. Hasegawa, M. Shirozu, I Kabe, C. Shimizu, N. Gonmori, A. Ogasawara, N. Kato, A. Tomizawa, N. Kunugita, T. Sone, K. Fukasawa, A. Hori, C. Nishiura, C. Kinugawa, R. Kuroda, K. Yamamoto, M. Ohtsu, N. Sakamoto, Y. Osaki, T. Totsuzaki, M. Endo, T. Itoh, M. Kawashima, M. Masuda, K. Kitahara, T. Yokoya, K. Fukai, K. Odagami, Y. Kobayashi
JOURNAL OF OCCUPATIONAL HEALTH
(2020)
Article
Environmental Sciences
Win-Yu Aung, Hironari Sakamoto, Ayana Sato, Ei-Ei-Pan-Nu Yi, Zaw-Lin Thein, Myint-San Nwe, Nanda Shein, Htin Linn, Shigehisa Uchiyama, Naoki Kunugita, Tin-Tin Win-Shwe, Ohn Mar
Summary: The study examined indoor formaldehyde concentrations in anatomy dissection rooms in Myanmar, finding that all rooms exceeded occupational exposure limits. Instructors had higher personal formaldehyde exposure levels than students, and students were more likely to experience formaldehyde-related symptoms during dissection sessions.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Medicine, General & Internal
Koichi Morota, Takashi Moritake, Keisuke Nagamoto, Satoru Matsuzaki, Koichi Nakagami, Lue Sun, Naoki Kunugita
Summary: Understanding the maximum skin dose in cerebral angiography is crucial for avoiding tissue reactions. This study developed a method using DICOM-RDSR data to accurately estimate the maximum skin dose from Total K-a,K-r. Results showed that using a quadratic function estimation model provided a more accurate estimate compared to a constant estimation model.
Article
Medicine, General & Internal
Koichi Nakagami, Takashi Moritake, Keisuke Nagamoto, Koichi Morota, Satoru Matsuzaki, Tomoko Kuriyama, Naoki Kunugita
Summary: The short curtain developed for over-table tube fluorographic imaging units showed a lower effectiveness in preventing cataracts compared to the long curtain, but demonstrated a significant improvement in providing radiation protection.
Article
Environmental Sciences
Hironari Sakamoto, Shigehisa Uchiyama, Tomohiko Isobe, Naoki Kunugita, Hironao Ogura, Shoji F. Nakayama
Summary: Research has found significant spatial variations in chemical concentrations within indoor spaces, affecting personal exposure among residents. The chemicals with the highest health risk were identified as acrolein, p-dichlorobenzene, and acetaldehyde in summer, and acrolein, nitrogen dioxide, formic acid, p-dichlorobenzene, and benzene in winter. The IAQ in spaces where residents spend a significant amount of time, such as the bedroom, has a significant impact on personal exposure.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Environmental Sciences
Takumi Yamaguchi, Itsuko Horiguchi, Naoki Kunugita
Summary: After the Fukushima nuclear accident, food products from affected areas faced reputational damage worldwide. This study aimed to identify the factors associated with people refraining from purchasing these foods and observed a decreasing trend in avoidance of Fukushima food products over time.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Ayumi Onoue, Yohei Inaba, Kentaro Machida, Takuya Samukawa, Hiromasa Inoue, Hajime Kurosawa, Hiromitsu Ogata, Naoki Kunugita, Hisamitsu Omori
Summary: Heated tobacco products (HTPs) have become increasingly popular among young smokers in Japan. However, there is limited research on the secondhand tobacco smoke (SHS) exposure due to HTPs. This study aimed to assess the urinary levels of total nicotine metabolites (TNMs) in non-smoking spouses and their children exposed to SHS from their fathers' HTP use. The findings showed that the spouses and children of fathers who used HTPs had significantly higher levels of TNMs in their urine compared to non-smokers or non-users. This study highlights the need for strategies to prevent SHS exposure from HTP use in public places and protect non-smokers from secondhand HTP aerosol exposure in households and other private settings.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Munehiko Kowataril, Keisuke Nagamoto, Koichi Nakagami, Hiroshi Yoshitomi, Takashi Moritake, Naoki Kunugita
Summary: The practical calibration method for a commercially available RPLD was demonstrated and its applicability for eye lens dose monitoring was verified through a series of experiments. The results showed good agreement between the RPLD and the DOSIRIS(TM) dosemeters, indicating its suitability for additional monitoring of the lens of the eyes for medical staff.
RADIATION PROTECTION DOSIMETRY
(2022)
Review
Biology
Sumi Yokoyama, Nobuyuki Hamada, Norio Tsujimura, Naoki Kunugita, Kazutaka Nishida, Iwao Ezaki, Masahiro Kato, Hideki Okubo
Summary: In April 2011, the International Commission on Radiological Protection recommended reducing the occupational equivalent dose limit for the lens. Japan implemented the new occupational lens dose limit in April 2021, and this paper reviews the updated information available by mid-2022, including regulatory implementation, discussions by relevant ministries, establishment of safety and health management systems, guidelines on lens dose monitoring and radiation safety, voluntary countermeasures, lens dose calibration method development, and recent studies on lens exposure in nuclear workers and biological effects.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Environmental Sciences
Keisuke Nagamoto, Ryo Watanabe, Tomonori Kawachino, Yoshitaka Ohishi, Aina Yamamoto, Takashi Moritake, Shun-ichi Nihei, Masayuki Kamochi, Naoki Kunugita
Summary: The purpose of this study was to evaluate the effectiveness of organ effect modulation (OEM) in reducing the lens dose in 4D CT imaging of the head. The results showed that OEM was effective in reducing the lens dose while ensuring image quality.
RADIATION PROTECTION DOSIMETRY
(2022)
Article
Environmental Sciences
Hironari Sakamoto, Shigehisa Uchiyama, Ayana Sato, Tomohiko Isobe, Naoki Kunugita, Hironao Ogura, Shoji F. Nakayama
Summary: This study investigated the personal exposure concentrations of various chemicals, including volatile organic compounds, acidic gases, and acrolein. The results showed that personal exposure concentrations were generally equal to or lower than concentrations in indoor air, contrary to previous studies. The high-risk chemicals identified based on personal exposure concentrations varied between seasons. Additionally, estimating personal exposure concentrations based on only these data proved to be difficult.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Satoru Matsuzaki, Takashi Moritake, Lue Sun, Koichi Morota, Keisuke Nagamoto, Koichi Nakagami, Tomoko Kuriyama, Go Hitomi, Shigeyuki Kajiki, Naoki Kunugita
Summary: This study aimed to explore practical measures to increase the usage of personal protective equipment (PPE) among interventional radiology (IR) physicians and reduce exposure doses. The intervention led to a significant improvement in the usage rate of PPE, but resulted in an increase in exposure doses.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Munehiko Kowatari, Takumi Kubota, Hiroshi Yoshitomi, Naoki Kunugita
Summary: We conducted an experiment to investigate the occupational exposure of radiation workers handling highly activated materials at a small research accelerator facility. Using a simplified physical phantom, we measured the personal dose equivalents at the eye lens and extremities of the workers and compared them with the doses measured by personal dosemeters on their trunk. Our results suggest that the eye lens doses can be estimated from the trunk dose, while the extremity doses vary depending on the geometry of the radiation source.
RADIATION PROTECTION DOSIMETRY
(2023)
Article
Public, Environmental & Occupational Health
Keisuke Nagamoto, Takashi Moritake, Koichi Nakagami, Koichi Morota, Satoru Matsuzaki, Naoki Kunugita
Summary: This study investigated the occupational dose to the lens of the eye for physicians engaged in radiology procedures in Japan, and proposed a multiple radiation protection protocol to ensure compliance with new-equivalent dose limits. The results indicated that multiple radiation protection measures were effective in reducing the eye lens dose received by physicians, showing a dose reduction rate of 53%.
JOURNAL OF OCCUPATIONAL HEALTH
(2021)
Article
Multidisciplinary Sciences
Keisuke Nagamoto, Takashi Moritake, Koichi Nakagami, Koichi Morota, Satoru Matsuzaki, Shun-ichi Nihei, Masayuki Kamochi, Naoki Kunugita
Summary: By introducing protective measures such as radiation safety glasses, bag-valve-mask extension tubes, and radiation protective curtains, the occupational dose to the lens of the eye for medical staff assisting in CT scans was significantly reduced, enabling compliance with the new equivalent dose limit without imposing significant burdens on physicians or medical institutions.