期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 11, 期 3, 页码 733-742出版社
WILEY
DOI: 10.1002/term.1970
关键词
drug delivery; peripheral nerve; chondroitinase ABC; GDNF; silk fibroin; nerve conduit
类别
资金
- US Department of Defense
- Armed Forces Institute of Regenerative Medicine (AFIRM)
- Plastic Surgery Foundation
Nerve conduits are a proven strategy for guiding axon regrowth following injury. This study compares degradable silk-trehalose films containing chondroitinase ABC (ChABC) and/or glial cell line-derived neurotrophic factor (GDNF) loaded within a silk fibroin-based nerve conduit in a rat sciatic nerve defect model. Four groups of silk conduits were prepared, with the following silk-trehalose films inserted into the conduit: (a) empty; (b) 1 mu g GDNF; (3) 2 U ChABC; and (4) 1 mu g GDNF/2 U ChABC. Drug release studies demonstrated 20% recovery of GDNFand ChABC at 6weeks and 24h, respectively. Six conduits of each type were implanted into 15mm sciatic nerve defects in Lewis rats; conduits were explanted for histological analysis at 6weeks. Tissues stained with Schwann cell S-100 antibody demonstrated an increased density of cells in both GDNF-and ChABC-treated groups compared to empty control conduits (p < 0.05). Conduits loaded with GDNF and ChABC also demonstrated higher levels of neuron-specific PGP 9.5 protein when compared to controls (p < 0.05). In this study we demonstrated a method to enhance Schwann cell migration and proliferation and also foster axonal regeneration when repairing peripheral nerve gap defects. Silk fibroin-based nerve conduits possess favourable mechanical and degradative properties and are further enhanced when loaded with ChABC and GDNF. Copyright (C) 2014 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据