Article
Biotechnology & Applied Microbiology
Yuriy Snyder, Soumen Jana
Summary: Efficient cell seeding and support from a substrate are crucial for tissue engineering, including heart valve tissue engineering. The use of fibrin gel as a cell carrier on trilayer PCL substrates can enhance cell proliferation and extracellular matrix production, leading to improved structure and mechanical properties of the tissue constructs. The gel also improves the orientation of cells and the produced tissue materials, making it highly beneficial for developing functional tissue-engineered leaflet constructs for heart valve tissue engineering.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Cell Biology
Sarah M. Somers, Warren L. Grayson
Summary: Bioreactor systems play a crucial role in tissue engineering by guiding cell differentiation and maturation. By combining with electrospun fibrin microfibers, the systems can be effectively applied in muscle tissue engineering. The system is also able to test the mechanical properties of hydrogel-based scaffolds and apply tensile strain for relevant experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Multidisciplinary Sciences
Nicholas Rogozinski, Apuleyo Yanez, Rahulkumar Bhoi, Moo-Yeal Lee, Huaxiao Yang
Summary: 3D cardiac engineered constructs not only advance cardiac regenerative medicine but also enable more accurate modeling of healthy and diseased cardiac tissues. This review highlights the relationship between different cell types and advanced fabrication methods, shedding light on various pathologies and providing therapeutic potential for damaged myocardium. The future direction is focused on creating specialized and personalized constructs with region-specific microtopography and function.
Article
Engineering, Biomedical
Jacqueline Bliley, Joshua Tashman, Maria Stang, Brian Coffin, Daniel Shiwarski, Andrew Lee, Thomas Hinton, Adam Feinberg
Summary: This study demonstrates the 3D bioprinting of a simplified heart model resembling embryonic development. The engineered heart tubes were cellularized and showed synchronous contractions similar to natural heart beats. These findings establish the feasibility of creating 3D bioprinted human heart tubes and open the door for engineering more complex heart muscle structures.
Article
Biotechnology & Applied Microbiology
Ruimin Long, Linrong Shi, Peng He, Jumei Tian, Shibin Wang, Jun Zheng
Summary: Using hydrogel as a filling medium to recombine dispersed microencapsulated cells and form an embedded gel-cell microcapsule complex is a novel approach for tissue construction. In this experiment, researchers used sodium alginate, chitosan, and silk fibroin to prepare different materials for the complex, and investigated the tissue-forming ability of the complex under different culture conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Thomas Eschenhagen, Katrin Ridders, Florian Weinberger
Summary: Heart regeneration is essential for treating heart disease by remuscularizing lost myocardium. While there has been progress in the past decade, there are still many important questions to be answered in order to optimize this approach for regenerative therapy of heart failure.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
WonJin Kim, GeunHyung Kim
Summary: A new cell-printing process is introduced in this study, which allows the fabrication of cell-spheroids and cell-loaded constructs together without the need to prepare cell-spheroids in advance. The diameter and location of spheroids can be manipulated by controlling printing parameters. Hybrid cell constructs fabricated using this method exhibit strong angiogenic and osteogenic activities.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Cardiac & Cardiovascular Systems
Yu-Chun Chang, Gabriel Mirhaidari, John Kelly, Christopher Breuer
Summary: Large-scale tissue engineering of cardiac constructs is a rapidly advancing field, but faces barriers in achieving successful electrochemical/mechanical cell coupling, efficient cardiomyocyte maturation, functional vascularization of large tissues, appropriate immune response, and large-scale generation of constructs. Promising solutions include the use of carbon/graphene, biological hormones, 3D bioprinting, and gene editing, although no single solution yet meets all criteria. It is important to consider a combination of techniques to optimize construct formation and address post-construction considerations such as good manufacturing practices and clinical trial standards.
CURRENT CARDIOLOGY REPORTS
(2021)
Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Article
Medicine, Research & Experimental
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Summary: This study introduces a new strategy of using hybrid structures composed of EC spheroids and hASCs for bone regeneration, which demonstrated outstanding angiogenic and osteogenic activities both in vitro and in vivo.
Article
Biotechnology & Applied Microbiology
Janine Tomasch, Babette Maleiner, Philipp Heher, Manuel Rufin, Orestis G. Andriotis, Philipp J. Thurner, Heinz Redl, Christiane Fuchs, Andreas H. Teuschl-Woller
Summary: This study aimed to investigate the influence of apparent elastic properties of fibrin scaffolds on myoblasts and evaluate if those effects differ between murine and human cells. The results showed that the elastic modulus of fibrin hydrogels had different effects on myoblast proliferation and differentiation, with a more pronounced impact on human cells in a 3D environment compared to 2D. Furthermore, the differentiation potential of human myoblasts was significantly impaired when incorporated into 3D gels. These findings provide insights into the cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches, highlighting the need for adjusting 3D culture parameters when working with human cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Tori Salem, Zachary Frankman, Jared M. Churko
Summary: This review explores important concepts and principles to consider when generating cardiovascular tissues from stem cell derived cardiomyocytes, as well as recent advancements in cardiac tissue engineering. Cardiac tissue engineering, involving complex engineering and physiological concepts, presents limitations in accessibility due to its interdisciplinary nature.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Review
Medicine, General & Internal
Chang Liu, Xing Feng, Guoping Li, Priyanka Gokulnath, Junjie Xiao
Summary: Human pluripotent stem cell (hPSC) technology provides abundant opportunities for disease modeling. Cardiomyocytes derived from hPSCs are efficient tools for studying cardiac diseases, drug screening, and pathological mechanisms. This review discusses the advantages and limitations of the 2D hPSC-cardiomyocyte system and introduces recent developments in 3D culture platforms derived from hPSCs.
Review
Engineering, Biomedical
Kenichi Arai, Takahiro Kitsuka, Koichi Nakayama
Summary: The safety and efficacy of new drugs are typically tested in animals, but differences in drug responses and potential cardiac side effects have led to many drug development projects being discontinued. Alternative methods to animal testing are needed. Tissue engineering techniques can create three-dimensional cardiac constructs from hiPSC-CMs, providing a new approach for drug screening.
Article
Biochemistry & Molecular Biology
Megane Beldjilali-Labro, Rachid Jellali, Alexander David Brown, Alejandro Garcia Garcia, Augustin Lerebours, Erwann Guenin, Fahmi Bedoui, Murielle Dufresne, Claire Stewart, Jean-Francois Grosset, Cecile Legallais
Summary: The goal of this study was to design and evaluate a multi-scale scaffold for promoting the differentiation of C2C12 skeletal myoblasts. The researchers found that the scaffold had tunable mechanical properties and improved the alignment and length of myotubes, leading to enhanced organization of reconstructed skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Nikita M. Patel, Ze-Wei Tao, Mohamed A. Mohamed, Matt K. Hogan, Laura Gutierez, Ravi K. Birla
Article
Engineering, Biomedical
Ze-Wei Tao, Mohamed Mohamed, Matthew Hogan, Betsy Salazar, Nikita M. Patel, Ravi K. Birla
Article
Cardiac & Cardiovascular Systems
Mohamed A. Mohamed, Matt K. Hogan, Nikita M. Patel, Ze-Wei Tao, Laura Gutierrez, Ravi K. Birla
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2015)
Article
Materials Science, Biomaterials
Matthew Hogan, Yi-Ting Chen, Arati G. Kolhatkar, Christopher J. Candelari, Sridhar Madala, T. Randall Lee, Ravi Birla
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2016)
Article
Materials Science, Biomaterials
Matthew Hogan, Yi-Ting Chen, Arati G. Kolhatkar, Christopher J. Candelari, Sridhar Madala, T. Randall Lee, Ravi Birla
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2016)
Article
Engineering, Biomedical
Matthew Hogan, Mohamed Mohamed, Ze-Wei Tao, Laura Gutierrez, Ravi Birla
Article
Engineering, Biomedical
Matthew Hogan, Glauco Souza, Ravi Birla
AIMS BIOENGINEERING
(2016)