Review
Polymer Science
Antony V. V. Samrot, Mahendran Sathiyasree, Sadiq Batcha Abdul Rahim, Robinson Emilin Renitta, Kasirajan Kasipandian, Sivasuriyan Krithika Shree, Deenadhayalan Rajalakshmi, Nagarajan Shobana, Shanmugaboopathi Dhiva, Sasi Abirami, Sridevi Visvanathan, Basanta Kumar Mohanty, Gokul Shankar Sabesan, Suresh V. Chinni
Summary: Biological macromolecules, such as polysaccharides, proteins, and glycoproteins, have been widely used in tissue engineering due to their ability to mimic the extracellular matrix and their biocompatibility. Scaffolds made from these macromolecules have properties like tensile strength, biodegradability, and flexibility. This review discusses the fabrication methods and applications of scaffolds using various biological macromolecules and emphasizes the importance of natural polymers over synthetic ones due to their unique properties.
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Elena De Angelis, Roberta Saleri, Paolo Martelli, Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Marta Pirola, Roberta Romeo, Melania Andrani, Valeria Cavalli, Virna Conti, Ruggero Bettini, Benedetta Passeri, Francesca Ravanetti, Paolo Borghetti
Summary: The study demonstrates that 3D printed CH scaffolds have good porosity for colonization and synthetic activity of chondrocytes. Enrichment with hyaluronic acid provides a more stable chondrocyte phenotype. The use of 3D CH scaffolds with PL instead of FBS in the medium results in increased gene expression of relevant ECM components.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biophysics
Deepak Patil, Sushma Kumari, Kaushik Chatterjee
Summary: This study presents an innovative spin-coating setup to generate nanotopography on 3D-printed polymer scaffolds, which exhibit excellent bactericidal and osteogenic activities. The nanostructured PLA surface showed a high bactericidal effect on P. aeruginosa and S. aureus, while also supporting pre-osteoblast attachment and proliferation. This work has important implications for engineering next-generation 3D printed bioactive tissue scaffolds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Biotechnology & Applied Microbiology
Xiaoshen Hu, Weiyang Zhang, Xiang Li, Dongling Zhong, Yuxi Li, Juan Li, Rongjiang Jin
Summary: Studies have shown that chondrocytes tend to dedifferentiate during cell amplification, losing their physiological properties, but recent research has made progress in redifferentiation control. By modulating various factors in cell culture, similar physiological characteristics and functions to human cartilage tissue have been achieved, offering hope for future cartilage repair and treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Angeliki Dimaraki, Pedro J. Diaz-Payno, Michelle Minneboo, Mahdiyeh Nouri-Goushki, Maryam Hosseini, Nicole Kops, Roberto Narcisi, Mohammad J. Mirzaali, Gerjo J. V. M. van Osch, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Summary: The study aimed to bioprint a scaffold with different zonal cell densities to mimic the organization of articular cartilage. Live/dead analysis and histological examination showed the ability to generate zonal cell density with high viability and smooth transition between zones, with higher glycosaminoglycan deposition in the highest cell density zone. These findings pave the way towards bioprinting complex zonal cartilage scaffolds as single units, advancing the translation of cartilage tissue engineering into clinical practice.
APPLIED SCIENCES-BASEL
(2021)
Article
Orthopedics
Nadine Frerker, Tommy A. Karlsen, Magnus Borstad Lilledahl, Sverre-Henning Brorson, John E. Tibballs, Jan E. Brinchmann
Summary: Scaffold-free engineering with BMP2 and allowing free movement in chondrogenic differentiation medium resulted in firm, elastic cartilage discs rich in type II collagen. This approach may have potential applications in clinical trials.
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Chemistry, Medicinal
Jiaxiang Gu, Bin Wang, Tianliang Wang, Naichen Zhang, Hongjun Liu, Jianchao Gui, Yiming Lu
Summary: In this study, the effects of cartilage progenitor cells (CPCs), bone marrow mesenchymal stem cells (BMSCs), and chondrocytes (CCs) on cartilage repair were investigated. The results showed that CPCs had higher proliferation ability compared to CCs and BMSCs. Moreover, CPCs seeded in agarose gel exhibited significantly higher levels of collagen, glycosaminoglycan, DNA content, and chondrocyte-related gene expression. Integration strength in the CPCs seeded gel was also higher than the other two gels. These findings suggest that CPCs have advantages over CCs and BMSCs as seed cells in tissue engineering for cartilage repair.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Cell & Tissue Engineering
Yongsheng Li, Wei Chen, Yao Dai, Yuting Huang, Zongming Chen, Tingfei Xi, Zheng Zhou, Hairong Liu
Summary: The study demonstrates that decellularized sturgeon cartilage ECM (dSCECM) scaffold can effectively inhibit chondrocyte hypertrophy, showing potential value in treating cartilage defects.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Polymer Science
Mohammad Javad Javid-Naderi, Javad Behravan, Negar Karimi-Hajishohreh, Shirin Toosi
Summary: Damage or loss of bone tissue is a major issue affecting human well-being, and tissue engineering is used to recover and regenerate bone fractures and damage. Polymer biomaterials are the most commonly required materials for synthetic bone scaffolds due to their suitable mechanical properties and degradation rates. Synthetic polymers offer advantages in bone replacement because they can be designed and changed easily, and their biocompatibility has been widely accepted in tissue engineering. This article updates on materials and application usage of scaffolds in bone tissue engineering for hard tissue regeneration.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2023)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Masoud Taghizadehjahed, Asma Sepahdar, Navid Rabiee, Abolfazl Nazbar, Saeed Farzad-Mohajeri, Mohammad Mehdi Dehghan, Mohammad Ali Shokrgozar, Mohammad Majidi, Seyed Hossein Mardjanmehr, Hossein Aminianfar, Hamid Akbari Javar, Shahin Bonakdar
Summary: In this study, a suitable substrate for cartilage lesions was synthesized by crosslinking polyvinyl alcohol hydrogel chains with polyurethane. The substrate was fully characterized and its efficacy was confirmed through in vitro and in vivo investigations using a sheep model. The results demonstrated that the isolated chondrocyte cultures on the synthesized substrate retained their original composition.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyi Lan, Yan Liang, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Tianxing Gong, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Skin cancer patients with tumorigenic lesions on their noses often require surgical resection resulting in nasal cartilage removal. Autologous tissue-engineered nasal cartilage grafts can help mitigate the problem, despite the issue of inhomogeneous distribution of cartilage matrix. Advances in 3D bioprinting technology offer customizable and anatomically shaped cartilages without the distribution issues.
Review
Chemistry, Multidisciplinary
Arun Kumar Rajendran, Hwan D. D. Kim, Jung-Whan Kim, Jong Woo Bae, Nathaniel S. S. Hwang
Summary: Nanotechnology has achieved unprecedented progress in the field of biomaterials science. Researchers in tissue engineering and regenerative medicine are extensively utilizing nanotechnology-based systems for targeted and triggered drug delivery, as well as enhancing tissue regeneration by mimicking nature with nanoscale structures. Technical advancements, such as photolithography, have enabled the incorporation of nanoscale features into existing biomaterials for bench-to-bedside applications. The development of gel systems, magnetic nanoparticle controllable systems, and spheroid and organoid cultures has further expanded the field. This review provides an overview of the various nanotechnological approaches being explored and utilized to enhance tissue regeneration.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Editorial Material
Biochemistry & Molecular Biology
Shima Tavakol, Alexander M. Seifalian
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Cell & Tissue Engineering
Shima Tavakol, Hani Tavakol, Mo S. Alavijeh, Alexander Seifalian
Summary: Nanomachines, including viruses, have been around for thousands of years. In 2019, the world had to come together to fight against SARS-CoV-2, a life-threatening nanomachine causing COVID-19. This research focuses on finding biomaterials that can inhibit the signaling pathways and ligand sites of the virus.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Review
Pharmacology & Pharmacy
Mehrnoush Mehrarya, Behnaz Gharehchelou, Samin Haghighi Poodeh, Elham Jamshidifar, Sara Karimifard, Bahareh Farasati Far, Iman Akbarzadeh, Alexander Seifalian
Summary: Infection is a disease caused by bacteria, and antibiotics are used for treatment. Delivering antibiotics to the infection site can prevent damage to the patient's body. Niosome nanoparticles, made from non-ionic surfactants, are ideal carriers for drug/antibiotic delivery and have shown effectiveness in treating local infections and delivering drugs.
JOURNAL OF DRUG TARGETING
(2022)
Article
Biochemistry & Molecular Biology
Mina Aleemardani, Pariya Zare, Amelia Seifalian, Zohreh Bagher, Alexander M. Seifalian
Summary: Peripheral nerve injury is a common medical condition impacting patient quality of life. Graphene-based materials show promise in neural tissue engineering to enhance nerve regeneration, with some products already in preclinical stages.
Review
Pharmacology & Pharmacy
Shima Tavakol, Hani Tavakol, Mo S. Alavijeh, Alexander Seifalian
Summary: This passage discusses the global efforts to combat the SARS-CoV-2 virus and the resulting COVID-19 illness in 2019. The variants of the virus, such as Alpha, Beta, Gamma, Delta, and Epsilon, show differences in cellular and molecular mechanisms, and they exhibit higher transmissibility rates and viral loads while evading neutralizing antibodies. New variants can be diagnosed using specific primers in the clinical laboratory, and there are controversial findings regarding the efficacy of vaccines against these variants.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Endocrinology & Metabolism
Shiva Hashemizadeh, Soheila Pourkhodadad, Saereh Hosseindoost, Sina Pejman, Maryam Kamarehei, Abolfazl Badripour, Ameneh Omidi, Seyed Khalil Pestehei, Alexander M. Seifalian, Mahmoudreza Hadjighassem
Summary: Damage to the spinal cord leads to inflammation and motor impairments. Inhibiting pro-inflammatory cells and activating anti-inflammatory cells may provide a potential treatment strategy. Ac-SDKP has protective effects, improving motor recovery, rescuing motor neurons, reducing inflammatory cytokine levels, and increasing anti-inflammatory gene expression.
Article
Biochemistry & Molecular Biology
Mercedeh Babaluei, Fatemeh Mottaghitalab, Alexander Seifalian, Mehdi Farokhi
Summary: This study developed an injectable hydrogel for wound dressings, containing sodium carboxymethylcellulose/polyacrylamide/polydopamine with vitamin C (CMC/PAAm/PDA VitC) and silk fibroin/alginate nanoparticles loaded with curcumin (SF/SANPs CUR). The hydrogels demonstrated stable properties, biocompatibility, and antibacterial activity against MRSA. In preclinical rat models, the hydrogels showed significant support for full-thickness burn regeneration, wound closure, and anti-inflammatory effects.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Elham Hasanzadeh, Alexander Seifalian, Amir Mellati, Jamileh Saremi, Shiva Asadpour, Seyed Ehsan Enderami, Houra Nekounam, Narges Mahmoodi
Summary: Repairing CNS is challenging due to the inability of neurons to recover. Injectable hydrogels have desirable attributes as biodegradable scaffolds for CNS tissue engineering and regeneration. They can imitate several aspects of CNS and are considered an effective therapeutic agent.
MATERIALS TODAY BIO
(2023)
Review
Biotechnology & Applied Microbiology
Mahsa Tashakori-Miyanroudi, Atousa Janzadeh, Alexander Seifalian, Nahid Aboutaleb, Yaser Azizi, Fatemeh Ramezani
Summary: Despite scientific advances, the number of cardiovascular patients is increasing worldwide. To protect damaged cardiomyocytes from further harm, novel and safer approaches are needed to help regeneration and prevent fibrosis. In this study, a systematic review of in vitro and preclinical studies of carbon nanotubes (CNTs) and carbon nanofibers (CNFs) for help to treat heart damage was conducted. The results showed that CNTs/CNFs in hydrogels increase conductivity, promote cardiac cell proliferation, and enhance expression of genes associated with stem cell differentiation into cardiac cells.
Article
Biochemistry & Molecular Biology
Marjan Mirhaj, Jaleh Varshosaz, Sheyda Labbaf, Rahmatollah Emadi, Alexander Marcus Seifalian, Fariborz Sharifianjazi, Mohamadreza Tavakoli
Summary: A core-shell nanofibrous wound dressing based on F127 containing mupirocin core and pectin-keratin shell was fabricated, which showed controlled release of mupirocin and improved wound healing compared to blended nanofibers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Neda Keyhanvar, Nosratollah Zarghami, Alexander Seifalian, Peyman Keyhanvar, Rana Sarvari, Roya Salehi, Reza Rahbarghazi, Mohammadreza Ranjkesh, Molood Akbarzadeh, Mahdi Mahdipour, Mohamamd Nouri
Summary: This study aimed to direct mesenchymal stem cells (MSCs) towards keratinocyte-like cells (KLCs) using cell imprinting and sheet technology. Culturing AT-MSCs on a PDMS substrate with keratinocyte cell-imprinted topography induced differentiation towards keratinocyte-like cells. The data suggests that the keratinocyte cell-imprinted substrate can provide a specific niche and topographical cues to orient AT-MSCs towards KLCs.
ADVANCED PHARMACEUTICAL BULLETIN
(2022)
Article
Dentistry, Oral Surgery & Medicine
Farshid Bastami, Mohammad-Hadi Noori-Kooshki, Hassan Semyari, Reza Tabrizi, Alireza Abrishamchian, Fatemeh Mashhadi-Abbas, Shahriar Shahab, Alexander Seifalian
Summary: The study evaluated the effects of MWCNT/HA granules with or without L-PRF on bone regeneration in a sheep model. Results showed that there was no significant inflammation around the granules, and new lamellar bone was regenerated. The addition of L-PRF to MWCNT/HA significantly improved new bone formation, while the use of MWCNT/HA granules alone also significantly increased new bone formation compared to control.
ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG
(2022)