Article
Biochemistry & Molecular Biology
Vadym Burchak, Fritz Koch, Leonard Siebler, Sonja Haase, Verena K. Horner, Xenia Kempter, G. Bjoern Stark, Ute Schepers, Alisa Grimm, Stefan Zimmermann, Peter Koltay, Sandra Strassburg, Gunter Finkenzeller, Filip Simunovic, Florian Lampert
Summary: This study evaluated the printability and biocompatibility of a semi-synthetic hydrogel system (GelNB/GelS) for bioprinting of adipose-tissue-derived mesenchymal stem cells (ASCs). The results showed that GelNB/GelS hydrogels supported ASCs viability, proliferation and differentiation. The mechanical properties of GelNB/GelS system influenced the differentiation fate of ASCs. The hydrogel system may have potential applications in in vivo tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Asma Sepahdar, Abolfazl Nazbar, Saeed Bahadorikhalili, Ghassem Rezaei, Mohammad Ali Shokrgozar, Mohammad Mehdi Dehghan, Hamid Akbari Javar, Shahin Bonakdar
Summary: This paper presents a novel composite scaffold for the regeneration of cartilage tissue. The scaffold is made of a combination of a hydrogel and a controlled release drug delivery system. The release of kartogenin, a chondrogenic-inducing drug, from the scaffold is successfully controlled. The scaffold also demonstrates a porous structure and shows good performance in inducing chondrogenic differentiation of stem cells.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Review
Cell Biology
Xueqi Wang, Yiming Guan, Shiyu Xiang, Karen L. Clark, Peter G. Alexander, Lauren E. Simonian, Yuhao Deng, Hang Lin
Summary: This review examines the potential of mesenchymal stromal cells (MSCs) in cartilage repair based on animal studies conducted in the past three decades. However, the cartilage tissue derived from MSCs does not possess the same characteristics as native hyaline cartilage, especially in terms of chondrocytic hypertrophy. This undesired phenotype needs to be controlled before MSCs can be effectively used for cartilage repair in clinical settings. Current research focuses on suppressing hypertrophy and modulating the Wnt/β-catenin pathway to inhibit hypertrophy, along with exploring the potential crosstalk between Wnt/β-catenin and other pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Engineering, Biomedical
Xiao Xu, Limei Xu, Jiang Xia, Caining Wen, Yujie Liang, Yuanmin Zhang
Summary: Osteoarthritis (OA) is a common disease characterized by cartilage degeneration in middle-aged and elderly people. Currently, there is no effective treatment for OA apart from total joint replacement in advanced stages. Mesenchymal stem cells (MSCs) have shown great potential in regulating the cartilage microenvironment, promoting cartilage regeneration, and alleviating OA symptoms, making them a promising source of cells for OA therapy.
ACTA BIOMATERIALIA
(2023)
Review
Chemistry, Medicinal
Gollahalli Shivashankar Prajwal, Naveen Jeyaraman, Krishna Kanth, Madhan Jeyaraman, Sathish Muthu, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Manish Khanna, Eun Jung Oh, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Tissue engineering and regenerative medicine provide a new approach for treating musculoskeletal diseases, utilizing MSCs and growth factors to improve the regenerative environment. However, the selection of MSCs source and the long-term and short-term effects of MSCs treatment remain uncertain.
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Jayavardini Vasanthan, Narasimman Gurusamy, Sheeja Rajasingh, Vinoth Sigamani, Shivaani Kirankumar, Edwin L. Thomas, Johnson Rajasingh
Summary: Mesenchymal stem cells (MSCs) are multipotent cells with the potential for tissue regeneration, can be easily isolated and cultured in vitro, and have been proven to be valuable for therapy. Quality control parameters, mechanisms of action, and applications in regenerative medicine and tissue engineering are important aspects to consider when utilizing MSCs.
Review
Biotechnology & Applied Microbiology
Xianggang Wang, Zuhao Li, Chenyu Wang, Haotian Bai, Zhonghan Wang, Yuzhe Liu, Yirui Bao, Ming Ren, He Liu, Jincheng Wang
Summary: The growth plate is vulnerable to damage with limited regenerative ability, leading to difficulties in achieving satisfactory outcomes with current clinical treatment strategies. Tissue engineering approaches, including biocompatible scaffolds with seed cells and growth factors, offer a promising alternative for GP repair and may be enhanced by advancements such as three-dimensional printing technology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Long Bai, Gang Tao, Maogeng Feng, Yuping Xie, Shuyu Cai, Shuanglin Peng, Jingang Xiao
Summary: With the advancement in understanding bone regeneration mechanisms and development of bone tissue engineering, a variety of scaffold carrier materials called hydrogels have emerged. Hydrogels are biocompatible, have unique swelling properties, and are relatively easy to fabricate, making them increasingly popular in bone regeneration and tissue engineering. Hydrogel drug delivery systems consist of cells, cytokines, an extracellular matrix, and small molecule nucleotides, each with different properties based on their chemical or physical cross-linking. This paper summarizes recent research in the field of bone regeneration using hydrogels as delivery carriers, discusses their application in bone defect diseases and their mechanisms, and explores future research directions of hydrogel drug delivery systems in bone tissue engineering.
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Mingjing Zhu, Wenchao Zhong, Wei Cao, Qingbin Zhang, Gang Wu
Summary: This review summarizes the current knowledge on the design, molecular mechanisms, and applications of peptides in cartilage tissue engineering. Peptides, which can be chemically synthesized and mimic the functions of cartilaginous extracellular matrix and growth factors, show better stability and modifiability compared to natural biomaterials and recombinant growth factors. Studies have shown that peptides have a good efficacy in inducing chondrogenesis.
BIOACTIVE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Simon F. Carroll, Conor T. Buckley, Daniel J. Kelly
Summary: This study investigated how factors such as cell source, environmental oxygen tension, and cell seeding density influence the local oxygen environment within engineered cartilaginous tissues, as well as their impact on cellular oxygen consumption rate and cartilage matrix synthesis. The findings suggest that promoting an oxygen consumption profile similar to that of chondrocytes may be a key factor in the success of stem cell-based cartilage tissue engineering strategies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
Nisa Irem Buyuk, Didem Aksu, Gamze Torun Kose
Summary: Combining PLA and 3D printer technology holds promise for personalized treatment of bone injuries and defects. A study found that scaffolds with a pore size of 370-460 μm showed the most ideal results in terms of cell retention, proliferation, and differentiation. 3D printed PLA scaffolds with a 60% filling rate were found to be promising for bone tissue engineering applications.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Spectroscopy
Hulya Aribuga, Utku Ertugral, Yusuf Alcay, Ozgur Yavuz, Mustafa Semih Yildirim, Emre Ozdemir, Kerem Kaya, Ayse Buse Ozdabak Sert, Fatma Nese Kok, Nurcan Senyurt Tuzun, Ismail Yilmaz
Summary: A new dual-channel probe MSB based on rhodamine B derivative was synthesized and characterized. It showed a fast, sensitive, and selective response to Fe3+ cation by enhancing UV-vis and fluorescence spectroscopy techniques. The color change of the probe solution from light yellow to pink in the presence of Fe3+ allowed for naked-eye determination. The probe was successfully applied to paper strips and real samples for the sensing of Fe3+ in aqueous solutions, with a detection limit of 4.85x10-9 M and a response time of 4 minutes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Materials Science, Ceramics
Senem Buyuksungur, Pinar Yilgor Huri, Juergen Schmidt, Iulian Pana, Mihaela Dinu, Catalin Vitelaru, Adrian E. Kiss, Dilara Goksu Tamay, Vasif Hasirci, Alina Vladescu, Nesrin Hasirci
Summary: Ti6Al4V alloy has been widely used as implant material in dental and orthopedic surgeries for its superior compatibility, low density, and corrosion resistance compared to other metals. Surface modification of the alloy is necessary to improve material-tissue interaction and bone integration. In this study, Ti6Al4V alloy surfaces were modified by RF magnetron sputtering and coated with zinc-doped hydroxyapatite. The resulting coating exhibited high stability, crystalline structure, enhanced corrosion resistance, osteointegration, and antimicrobial effectiveness against Escherichia coli (E. coli) bacteria.
CERAMICS INTERNATIONAL
(2023)
Article
Materials Science, Biomaterials
Ezgi Antmen, Menekse Ermis, Ozgur Kuren, Kemal Beksac, Cigdem Irkkan, Vasif Hasirci
Summary: In this study, the combination of conventional molecular-level evaluations and in vitro methods on micropatterned biomaterial surfaces was used to assess breast adenocarcinoma patients. The results showed that molecular markers and shape descriptors could distinguish cells from different locations and patients. Cells with the highest metastatic and invasive properties exhibited both the highest deformability and the highest level of metastatic markers. By combining molecular markers with nuclear deformation, the detection and separation of subpopulations in heterogeneous breast cancer specimens at the single-cell level can be improved.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Ilayda Duru, Nisa Irem Buyuk, Gamze Torun Kose, Dylan Widder Marques, Karina Ann Bruce, John Robert Martin, Duygu Ege
Summary: In this study, fullerenol (Ful), a water-soluble derivative of C-60 fullerene with antioxidant activity, is incorporated into calcium phosphate cements (CPCs) to enhance their osteogenic ability. The addition of Ful in CPCs results in improved mechanical performance, antioxidant activity, and cellular osteogenesis. The results suggest that Ful-loaded cements can be a promising additive for bone reconstruction procedures.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Engineering, Biomedical
Kenda Sabouni, Yetkin Ozturk, Erkan Kacar, Gamze Torun Kose, Fatma Nese Kok, Muhammet Kursat Kazmanli, Mustafa Kamil Urgen, Sakip Onder
Summary: In this study, the effect of (Ti, Mg)N thin film coated Ti6Al4V plates and screws on mineralization/osseointegration was investigated. The results showed that the Mg-containing coatings enhanced mineralization, cell attachment, and hydroxyapatite growth, leading to increased attachment at the implant tissue interface.
BIO-MEDICAL MATERIALS AND ENGINEERING
(2023)
Article
Engineering, Biomedical
Mervesu Gokyurek, Sumeyra Guler, Seyda Gokyer, Nuray Yazihan, Melih Akinci, Mehmet Ali Gulcelik, Kerim Bora Yilmaz, Pinar Yilgor
Summary: The parathyroid glands regulate the calcium level in the blood and can be damaged during thyroid surgery. Tissue engineering, particularly 3D printing, offers a potential solution for regenerating or replacing malfunctioning parathyroid tissue. In this study, human primary parathyroid cells were successfully isolated and seeded onto 3D printed alginate scaffolds, resulting in the engineering of functional hormone-active parathyroid tissue in vitro.
BIOMEDICAL MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Cigdem Buse Oral, Berkant Yetiskin, Canan Cil, Fatma Nese Kok, Oguz Okay
Summary: Inspired by nature, we designed organohydrogels (OHGs) consisting of a silk fibroin (SF) hydrogel as the continuous phase and the hydrophobic microinclusions based on semicrystalline poly(n-octadecyl acrylate) (PC18A) as the dispersed phase. SF acts as a self-emulsifier to obtain oil-in-water emulsions, and ethanol is added to induce gelation in the continuous SF phase. In the second step, in situ polymerization of C18A droplets in the emulsion system was conducted under UV light in the presence of a photoinitiator to obtain high-strength OHGs with shape-memory function and good cytocompatibility.
ACS APPLIED BIO MATERIALS
(2023)
Article
Polymer Science
Dilruba Baykara, Esra Pilavci, Songul Ulag, Oseweuba Valentine Okoro, Lei Nie, Amin Shavandi, Ayse Ceren Koyuncu, Ozlem Bingol Ozakpinar, Mehmet Eroglu, Oguzhan Gunduz
Summary: The aim of this study was to develop a 3D-printed wound dressing that can release amoxicillin in response to electrical stimulation. This was achieved by incorporating bismuth ferrite nanoparticles into a chitosan-based scaffold. The results showed that the scaffold had a suitable structure for drug loading and release, and the release of amoxicillin was successfully controlled by electrical stimulation.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Gorkem Cemali, Begum Okutan, Erde Can Safak, Gamze Torun Kose
Summary: Poly(propylene fumarate) (PPF) was synthesized and thermally cured with different weight ratios of vinylphosphonic acid (VPA) and diethyl vinylphosphonate (VPES). The biocompatibility and bone formation capacity of the PPF copolymers were evaluated for their potential as bone tissue scaffolds. The results showed that PPF/VPA copolymers had better cell attachment and higher ALP activity compared to PPF/VPES copolymers, indicating their suitability for bone regeneration.
JOURNAL OF POLYMER RESEARCH
(2023)
Article
Chemistry, Analytical
Yusuf Alcay, Emre Ozdemir, Mustafa Semih Yildirim, Utku Ertugral, Ozgur Yavuz, Hulya Aribuga, Yilmaz Ozkilic, Nurcan Senyurt Tuzun, Ayse Buse Ozdabak Sert, Fatma Nese Kok, Ismail Yilmaz
Summary: This study aimed to construct a new NIR fluorophore probe (CSME) based on a chromenylium cyanine platform decorated with methionine biomolecule, to address the shortcomings in the analysis of hazardous Hg2+ in the environment and living cells. The CSME structure and its interaction with Hg2+ ion were assessed using various techniques, and the probe showed significant changes in spectral and colorimetric properties upon interaction with Hg2+. The probe exhibited fast response time, wide linear ranges, and a lower Limit of Detection (LOD) compared to the US Environmental Protection Agency (EPA) prediction. It was successfully applied for Hg2+ analysis in drinking water, tap water, and living cells.
Article
Chemistry, Multidisciplinary
Ayse Buse Ozdabak Sert, Eva Bittrich, Petra Uhlmann, Fatma Nese Kok, Abdulhalim Kilic
Summary: A study using QCM-D technique was conducted to monitor the adhesion of human fetal osteoblastic cells on PCL and CH polymer films. The results showed that the adsorbed amount of fibrinogen and the cell sedimentation decreased with an increasing ratio of chitosan. QCM-D signals also reflected the different interfacial properties of the homopolymer and blend films.
Article
Nanoscience & Nanotechnology
Cigdem Buse Oral, Berkant Yetiskin, Canan Cil, Fatma Nese Kok, Oguz Okay
Summary: Inspired by nature, the researchers designed organohydrogels (OHGs) using silk fibroin (SF) as the continuous phase and semicrystalline poly(n-octadecyl acrylate) (PC18A) as the dispersed phase. SF acted as a self-emulsifier, making it a versatile and green alternative to chemical emulsifiers. By inducing gelation in the SF phase and polymerization of PC18A droplets, the researchers obtained high-strength OHGs with shape-memory function and good cytocompatibility. The incorporation of hydrophilic and noncrystallizable hydrophobic units further improved the mechanical and shape-memory properties of the OHGs.
ACS APPLIED BIO MATERIALS
(2023)
Article
Automation & Control Systems
Gorkem Liman, Emre Ergene, Emrecan Yildiz, Kubra Ozkan Hukum, Pinar Yilgor Huri, Arif E. Cetin, Hakan Usta, Gokhan Demirel
Summary: By decorating plasmonic nanoparticles on magnetic actuators, we demonstrate a new soft actuator platform that can generate reversible and tunable hot-spots in millimeter-sized areas via bending motion. The hot-spot formation is shown to be reversible and adjustable, with Raman signal enhancements of up to approximately 120 folds compared to the unactuated platforms. The accessible electromagnetic field magnification in the platform can be manipulated by controlling magnetic field strength. Additionally, a centipede-inspired robot is fabricated and used for sample collection/analysis in a target environment.
ADVANCED INTELLIGENT SYSTEMS
(2023)
